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Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression

Altered expression levels of N-methyl-D-aspartate receptor (NMDAR), a ligand-gated ion channel, have a harmful effect on cellular survival. Hyperthermia is a proven risk factor of transient forebrain ischemia (tFI) and can cause extensive and severe brain damage associated with mortality. The object...

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Autores principales: Kim, Bora, Ahn, Ji Hyeon, Kim, Dae Won, Lee, Tae-Kyeong, Kim, Yoon Sung, Shin, Myoung Cheol, Cho, Jun Hwi, Kim, Young-Myeong, Park, Joon Ha, Kang, Il Jun, Lee, Jae-Chul, Won, Moo-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893780/
https://www.ncbi.nlm.nih.gov/pubmed/33537826
http://dx.doi.org/10.3892/mmr.2021.11895
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author Kim, Bora
Ahn, Ji Hyeon
Kim, Dae Won
Lee, Tae-Kyeong
Kim, Yoon Sung
Shin, Myoung Cheol
Cho, Jun Hwi
Kim, Young-Myeong
Park, Joon Ha
Kang, Il Jun
Lee, Jae-Chul
Won, Moo-Ho
author_facet Kim, Bora
Ahn, Ji Hyeon
Kim, Dae Won
Lee, Tae-Kyeong
Kim, Yoon Sung
Shin, Myoung Cheol
Cho, Jun Hwi
Kim, Young-Myeong
Park, Joon Ha
Kang, Il Jun
Lee, Jae-Chul
Won, Moo-Ho
author_sort Kim, Bora
collection PubMed
description Altered expression levels of N-methyl-D-aspartate receptor (NMDAR), a ligand-gated ion channel, have a harmful effect on cellular survival. Hyperthermia is a proven risk factor of transient forebrain ischemia (tFI) and can cause extensive and severe brain damage associated with mortality. The objective of the present study was to investigate whether hyperthermic preconditioning affected NMDAR1 immunoreactivity associated with deterioration of neuronal function in the gerbil hippocampal CA1 region following tFI via histological and western blot analyses. Hyperthermic preconditioning was performed for 1 h before tFI, which was developed by ligating common carotid arteries for 5 min. tFI-induced cognitive impairment under hyperthermia was worse compared with that under normothermia. Loss (death) of pyramidal neurons in the CA1 region occurred fast and was more severe under hyperthermia compared with that under normothermia. NMDAR1 immunoreactivity was not observed in the somata of pyramidal neurons of sham gerbils with normothermia. However, its immunoreactivity was strong in the somata and processes at 12 h post-tFI. Thereafter, NMDAR1 immunoreactivity decreased with time after tFI. On the other hand, NMDAR1 immunoreactivity under hyperthermia was significantly increased in the somata and processes at 6 h post-tFI. The change pattern of NMDAR1 immunoreactivity under hyperthermia was different from that under normothermia. Overall, accelerated tFI-induced neuronal death under hyperthermia may be closely associated with altered NMDAR1 expression compared with that under normothermia.
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spelling pubmed-78937802021-03-08 Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression Kim, Bora Ahn, Ji Hyeon Kim, Dae Won Lee, Tae-Kyeong Kim, Yoon Sung Shin, Myoung Cheol Cho, Jun Hwi Kim, Young-Myeong Park, Joon Ha Kang, Il Jun Lee, Jae-Chul Won, Moo-Ho Mol Med Rep Articles Altered expression levels of N-methyl-D-aspartate receptor (NMDAR), a ligand-gated ion channel, have a harmful effect on cellular survival. Hyperthermia is a proven risk factor of transient forebrain ischemia (tFI) and can cause extensive and severe brain damage associated with mortality. The objective of the present study was to investigate whether hyperthermic preconditioning affected NMDAR1 immunoreactivity associated with deterioration of neuronal function in the gerbil hippocampal CA1 region following tFI via histological and western blot analyses. Hyperthermic preconditioning was performed for 1 h before tFI, which was developed by ligating common carotid arteries for 5 min. tFI-induced cognitive impairment under hyperthermia was worse compared with that under normothermia. Loss (death) of pyramidal neurons in the CA1 region occurred fast and was more severe under hyperthermia compared with that under normothermia. NMDAR1 immunoreactivity was not observed in the somata of pyramidal neurons of sham gerbils with normothermia. However, its immunoreactivity was strong in the somata and processes at 12 h post-tFI. Thereafter, NMDAR1 immunoreactivity decreased with time after tFI. On the other hand, NMDAR1 immunoreactivity under hyperthermia was significantly increased in the somata and processes at 6 h post-tFI. The change pattern of NMDAR1 immunoreactivity under hyperthermia was different from that under normothermia. Overall, accelerated tFI-induced neuronal death under hyperthermia may be closely associated with altered NMDAR1 expression compared with that under normothermia. D.A. Spandidos 2021-04 2021-02-04 /pmc/articles/PMC7893780/ /pubmed/33537826 http://dx.doi.org/10.3892/mmr.2021.11895 Text en Copyright: © Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Kim, Bora
Ahn, Ji Hyeon
Kim, Dae Won
Lee, Tae-Kyeong
Kim, Yoon Sung
Shin, Myoung Cheol
Cho, Jun Hwi
Kim, Young-Myeong
Park, Joon Ha
Kang, Il Jun
Lee, Jae-Chul
Won, Moo-Ho
Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression
title Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression
title_full Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression
title_fullStr Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression
title_full_unstemmed Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression
title_short Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression
title_sort transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing nmdar1 expression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893780/
https://www.ncbi.nlm.nih.gov/pubmed/33537826
http://dx.doi.org/10.3892/mmr.2021.11895
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