Cargando…
Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression
Altered expression levels of N-methyl-D-aspartate receptor (NMDAR), a ligand-gated ion channel, have a harmful effect on cellular survival. Hyperthermia is a proven risk factor of transient forebrain ischemia (tFI) and can cause extensive and severe brain damage associated with mortality. The object...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893780/ https://www.ncbi.nlm.nih.gov/pubmed/33537826 http://dx.doi.org/10.3892/mmr.2021.11895 |
_version_ | 1783653116190654464 |
---|---|
author | Kim, Bora Ahn, Ji Hyeon Kim, Dae Won Lee, Tae-Kyeong Kim, Yoon Sung Shin, Myoung Cheol Cho, Jun Hwi Kim, Young-Myeong Park, Joon Ha Kang, Il Jun Lee, Jae-Chul Won, Moo-Ho |
author_facet | Kim, Bora Ahn, Ji Hyeon Kim, Dae Won Lee, Tae-Kyeong Kim, Yoon Sung Shin, Myoung Cheol Cho, Jun Hwi Kim, Young-Myeong Park, Joon Ha Kang, Il Jun Lee, Jae-Chul Won, Moo-Ho |
author_sort | Kim, Bora |
collection | PubMed |
description | Altered expression levels of N-methyl-D-aspartate receptor (NMDAR), a ligand-gated ion channel, have a harmful effect on cellular survival. Hyperthermia is a proven risk factor of transient forebrain ischemia (tFI) and can cause extensive and severe brain damage associated with mortality. The objective of the present study was to investigate whether hyperthermic preconditioning affected NMDAR1 immunoreactivity associated with deterioration of neuronal function in the gerbil hippocampal CA1 region following tFI via histological and western blot analyses. Hyperthermic preconditioning was performed for 1 h before tFI, which was developed by ligating common carotid arteries for 5 min. tFI-induced cognitive impairment under hyperthermia was worse compared with that under normothermia. Loss (death) of pyramidal neurons in the CA1 region occurred fast and was more severe under hyperthermia compared with that under normothermia. NMDAR1 immunoreactivity was not observed in the somata of pyramidal neurons of sham gerbils with normothermia. However, its immunoreactivity was strong in the somata and processes at 12 h post-tFI. Thereafter, NMDAR1 immunoreactivity decreased with time after tFI. On the other hand, NMDAR1 immunoreactivity under hyperthermia was significantly increased in the somata and processes at 6 h post-tFI. The change pattern of NMDAR1 immunoreactivity under hyperthermia was different from that under normothermia. Overall, accelerated tFI-induced neuronal death under hyperthermia may be closely associated with altered NMDAR1 expression compared with that under normothermia. |
format | Online Article Text |
id | pubmed-7893780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-78937802021-03-08 Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression Kim, Bora Ahn, Ji Hyeon Kim, Dae Won Lee, Tae-Kyeong Kim, Yoon Sung Shin, Myoung Cheol Cho, Jun Hwi Kim, Young-Myeong Park, Joon Ha Kang, Il Jun Lee, Jae-Chul Won, Moo-Ho Mol Med Rep Articles Altered expression levels of N-methyl-D-aspartate receptor (NMDAR), a ligand-gated ion channel, have a harmful effect on cellular survival. Hyperthermia is a proven risk factor of transient forebrain ischemia (tFI) and can cause extensive and severe brain damage associated with mortality. The objective of the present study was to investigate whether hyperthermic preconditioning affected NMDAR1 immunoreactivity associated with deterioration of neuronal function in the gerbil hippocampal CA1 region following tFI via histological and western blot analyses. Hyperthermic preconditioning was performed for 1 h before tFI, which was developed by ligating common carotid arteries for 5 min. tFI-induced cognitive impairment under hyperthermia was worse compared with that under normothermia. Loss (death) of pyramidal neurons in the CA1 region occurred fast and was more severe under hyperthermia compared with that under normothermia. NMDAR1 immunoreactivity was not observed in the somata of pyramidal neurons of sham gerbils with normothermia. However, its immunoreactivity was strong in the somata and processes at 12 h post-tFI. Thereafter, NMDAR1 immunoreactivity decreased with time after tFI. On the other hand, NMDAR1 immunoreactivity under hyperthermia was significantly increased in the somata and processes at 6 h post-tFI. The change pattern of NMDAR1 immunoreactivity under hyperthermia was different from that under normothermia. Overall, accelerated tFI-induced neuronal death under hyperthermia may be closely associated with altered NMDAR1 expression compared with that under normothermia. D.A. Spandidos 2021-04 2021-02-04 /pmc/articles/PMC7893780/ /pubmed/33537826 http://dx.doi.org/10.3892/mmr.2021.11895 Text en Copyright: © Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Kim, Bora Ahn, Ji Hyeon Kim, Dae Won Lee, Tae-Kyeong Kim, Yoon Sung Shin, Myoung Cheol Cho, Jun Hwi Kim, Young-Myeong Park, Joon Ha Kang, Il Jun Lee, Jae-Chul Won, Moo-Ho Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression |
title | Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression |
title_full | Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression |
title_fullStr | Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression |
title_full_unstemmed | Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression |
title_short | Transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing NMDAR1 expression |
title_sort | transient forebrain ischemia under hyperthermic condition accelerates memory impairment and neuronal death in the gerbil hippocampus by increasing nmdar1 expression |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893780/ https://www.ncbi.nlm.nih.gov/pubmed/33537826 http://dx.doi.org/10.3892/mmr.2021.11895 |
work_keys_str_mv | AT kimbora transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT ahnjihyeon transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT kimdaewon transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT leetaekyeong transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT kimyoonsung transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT shinmyoungcheol transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT chojunhwi transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT kimyoungmyeong transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT parkjoonha transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT kangiljun transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT leejaechul transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression AT wonmooho transientforebrainischemiaunderhyperthermicconditionacceleratesmemoryimpairmentandneuronaldeathinthegerbilhippocampusbyincreasingnmdar1expression |