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Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway

BACKGROUND: Mechanisms that preclude lung metastasis are still barely understood. The possible consequences of allergic airways inflammation on cancer dissemination were studied in a mouse model of breast cancer. METHODS: Balb/c mice were immunized and daily exposed to ovalbumin (OVA) from day 21. T...

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Autores principales: Bekaert, S., Rocks, N., Vanwinge, C., Noel, A., Cataldo, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893955/
https://www.ncbi.nlm.nih.gov/pubmed/33608009
http://dx.doi.org/10.1186/s12931-021-01652-9
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author Bekaert, S.
Rocks, N.
Vanwinge, C.
Noel, A.
Cataldo, D.
author_facet Bekaert, S.
Rocks, N.
Vanwinge, C.
Noel, A.
Cataldo, D.
author_sort Bekaert, S.
collection PubMed
description BACKGROUND: Mechanisms that preclude lung metastasis are still barely understood. The possible consequences of allergic airways inflammation on cancer dissemination were studied in a mouse model of breast cancer. METHODS: Balb/c mice were immunized and daily exposed to ovalbumin (OVA) from day 21. They were subcutaneously injected with 4T1 mammary tumor cells on day 45 and sacrificed on day 67. Lung metastases were measured by biophotonic imaging (IVIS® 200 Imaging System) and histological measurement of tumor area (Cytomine software). Effects of CCL11 were assessed in vivo by intratracheal instillations of recCCL11 and in vitro using Boyden chambers. CCR3 expression on cell surface was assessed by flow cytometry. RESULTS: The extent of tumor metastases was significantly higher in lungs of OVA-exposed mice and increased levels of CCL11 expression were measured after OVA exposure. Migration of 4T1 cells and neutrophils was stimulated in vitro and in vivo by recCCL11. 4T1 cells and neutrophils express CCR3 as shown by flow cytometry and a selective CCR3 antagonist (SB-297006) inhibited the induction of 4T1 cells migration and proliferation in response to recCCL11. CONCLUSIONS: Allergic inflammation generated by exposure to allergens triggers the implantation of metastatic cells from primary breast tumor into lung tissues plausibly in a CCL11–CCR3-dependent manner. This indicates that asthma related inflammation in lungs might be a risk factor for lung metastasis in breast cancer patients.
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spelling pubmed-78939552021-02-22 Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway Bekaert, S. Rocks, N. Vanwinge, C. Noel, A. Cataldo, D. Respir Res Research BACKGROUND: Mechanisms that preclude lung metastasis are still barely understood. The possible consequences of allergic airways inflammation on cancer dissemination were studied in a mouse model of breast cancer. METHODS: Balb/c mice were immunized and daily exposed to ovalbumin (OVA) from day 21. They were subcutaneously injected with 4T1 mammary tumor cells on day 45 and sacrificed on day 67. Lung metastases were measured by biophotonic imaging (IVIS® 200 Imaging System) and histological measurement of tumor area (Cytomine software). Effects of CCL11 were assessed in vivo by intratracheal instillations of recCCL11 and in vitro using Boyden chambers. CCR3 expression on cell surface was assessed by flow cytometry. RESULTS: The extent of tumor metastases was significantly higher in lungs of OVA-exposed mice and increased levels of CCL11 expression were measured after OVA exposure. Migration of 4T1 cells and neutrophils was stimulated in vitro and in vivo by recCCL11. 4T1 cells and neutrophils express CCR3 as shown by flow cytometry and a selective CCR3 antagonist (SB-297006) inhibited the induction of 4T1 cells migration and proliferation in response to recCCL11. CONCLUSIONS: Allergic inflammation generated by exposure to allergens triggers the implantation of metastatic cells from primary breast tumor into lung tissues plausibly in a CCL11–CCR3-dependent manner. This indicates that asthma related inflammation in lungs might be a risk factor for lung metastasis in breast cancer patients. BioMed Central 2021-02-19 2021 /pmc/articles/PMC7893955/ /pubmed/33608009 http://dx.doi.org/10.1186/s12931-021-01652-9 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Bekaert, S.
Rocks, N.
Vanwinge, C.
Noel, A.
Cataldo, D.
Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway
title Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway
title_full Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway
title_fullStr Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway
title_full_unstemmed Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway
title_short Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway
title_sort asthma-related inflammation promotes lung metastasis of breast cancer cells through ccl11–ccr3 pathway
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893955/
https://www.ncbi.nlm.nih.gov/pubmed/33608009
http://dx.doi.org/10.1186/s12931-021-01652-9
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