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FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants

The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at...

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Detalles Bibliográficos
Autores principales: Long, Yanping, Liu, Zhijian, Jia, Jinbu, Mo, Weipeng, Fang, Liang, Lu, Dongdong, Liu, Bo, Zhang, Hong, Chen, Wei, Zhai, Jixian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893963/
https://www.ncbi.nlm.nih.gov/pubmed/33608047
http://dx.doi.org/10.1186/s13059-021-02288-0
Descripción
Sumario:The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at a single-nucleus level in plants using isolated nuclei. Combined with 10x Genomics and Nanopore long-read sequencing, we validate the robustness of this approach in Arabidopsis root cells and the developing endosperm. Sequencing results demonstrate that it allows for uncovering alternative splicing and polyadenylation-related RNA isoform information at the single-cell level, which facilitates characterizing cell identities. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13059-021-02288-0.