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Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model
Recently, the feasibility of real‐time indocyanine green (ICG) fluorescence imaging–guided complete mesocolic excision in colon cancer surgery has been demonstrated; however, its application to the evaluation of lymphatic flow in widespread lymph node metastasis is uncertain. This study aimed to eva...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894008/ http://dx.doi.org/10.1111/cas.14766 |
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author | Yamamoto, Masashi Taniguchi, Kohei Tominaga, Tomo Shibata, Masa‐Aki Inomata, Yosuke Komura, Kazumasa Osumi, Wataru Hamamoto, Hiroki Tanaka, Keitaro Okuda, Junji Uchiyama, Kazuhisa |
author_facet | Yamamoto, Masashi Taniguchi, Kohei Tominaga, Tomo Shibata, Masa‐Aki Inomata, Yosuke Komura, Kazumasa Osumi, Wataru Hamamoto, Hiroki Tanaka, Keitaro Okuda, Junji Uchiyama, Kazuhisa |
author_sort | Yamamoto, Masashi |
collection | PubMed |
description | Recently, the feasibility of real‐time indocyanine green (ICG) fluorescence imaging–guided complete mesocolic excision in colon cancer surgery has been demonstrated; however, its application to the evaluation of lymphatic flow in widespread lymph node metastasis is uncertain. This study aimed to evaluate lymphatic flow using the real‐time ICG fluorescence imaging. A mouse model of subcutaneous inoculation of BJMC3879Luc2 cells, which have been demonstrated to highly metastasize to the lymph nodes, was used as an evaluation model. Tumor growth and lymphatic flow were monitored weekly by bioluminescent imaging and near‐infrared (NIR) fluorescence imaging, respectively. After sacrificing the mice, lymph node metastases were evaluated by bioluminescent imaging and histopathology. Lymphatic flows in a model of high lymph node metastasis were evaluated using NIR fluorescence imaging. Pathological metastases of bilateral axillary, femoral, and para‐aortic lymph nodes were detected in all inoculated mice (100%: 5/5). Real‐time NIR fluorescence imaging showed the primary lymphatic vessels staining through the metastatic lymph nodes as before the inoculation of the cancer cells. Hitherto, it has been considered that lymphatic flow was changed using the bypass pathway due to occlusion of the primary lymphatic vessels. In this presented study, real‐time ICG fluorescence imaging showed no changes in lymphatic flow after lymph node metastasis. Our results suggest that real‐time ICG fluorescence imaging may have potential for the guidance of colon cancer surgery in cases of widespread lymph node metastasis. |
format | Online Article Text |
id | pubmed-7894008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78940082021-03-02 Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model Yamamoto, Masashi Taniguchi, Kohei Tominaga, Tomo Shibata, Masa‐Aki Inomata, Yosuke Komura, Kazumasa Osumi, Wataru Hamamoto, Hiroki Tanaka, Keitaro Okuda, Junji Uchiyama, Kazuhisa Cancer Sci Original Articles Recently, the feasibility of real‐time indocyanine green (ICG) fluorescence imaging–guided complete mesocolic excision in colon cancer surgery has been demonstrated; however, its application to the evaluation of lymphatic flow in widespread lymph node metastasis is uncertain. This study aimed to evaluate lymphatic flow using the real‐time ICG fluorescence imaging. A mouse model of subcutaneous inoculation of BJMC3879Luc2 cells, which have been demonstrated to highly metastasize to the lymph nodes, was used as an evaluation model. Tumor growth and lymphatic flow were monitored weekly by bioluminescent imaging and near‐infrared (NIR) fluorescence imaging, respectively. After sacrificing the mice, lymph node metastases were evaluated by bioluminescent imaging and histopathology. Lymphatic flows in a model of high lymph node metastasis were evaluated using NIR fluorescence imaging. Pathological metastases of bilateral axillary, femoral, and para‐aortic lymph nodes were detected in all inoculated mice (100%: 5/5). Real‐time NIR fluorescence imaging showed the primary lymphatic vessels staining through the metastatic lymph nodes as before the inoculation of the cancer cells. Hitherto, it has been considered that lymphatic flow was changed using the bypass pathway due to occlusion of the primary lymphatic vessels. In this presented study, real‐time ICG fluorescence imaging showed no changes in lymphatic flow after lymph node metastasis. Our results suggest that real‐time ICG fluorescence imaging may have potential for the guidance of colon cancer surgery in cases of widespread lymph node metastasis. John Wiley and Sons Inc. 2020-12-22 2021-02 /pmc/articles/PMC7894008/ http://dx.doi.org/10.1111/cas.14766 Text en © 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Yamamoto, Masashi Taniguchi, Kohei Tominaga, Tomo Shibata, Masa‐Aki Inomata, Yosuke Komura, Kazumasa Osumi, Wataru Hamamoto, Hiroki Tanaka, Keitaro Okuda, Junji Uchiyama, Kazuhisa Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model |
title | Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model |
title_full | Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model |
title_fullStr | Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model |
title_full_unstemmed | Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model |
title_short | Evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model |
title_sort | evaluation of lymphatic flow pattern using indocyanine green fluorescence imaging in a highly metastatic mouse model |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894008/ http://dx.doi.org/10.1111/cas.14766 |
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