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Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize

Chlorophylls, green pigments in chloroplasts, are essential for photosynthesis. Reduction in chlorophyll content may result in retarded growth, dwarfism, and sterility. In this study, a yellow-green leaf mutant of maize, indicative of abnormity in chlorophyll content, was identified. The physiologic...

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Autores principales: Li, Tingchun, Yang, Huaying, Lu, Yan, Dong, Qing, Liu, Guihu, Chen, Feng, Zhou, Yingbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894110/
https://www.ncbi.nlm.nih.gov/pubmed/33628629
http://dx.doi.org/10.7717/peerj.10567
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author Li, Tingchun
Yang, Huaying
Lu, Yan
Dong, Qing
Liu, Guihu
Chen, Feng
Zhou, Yingbing
author_facet Li, Tingchun
Yang, Huaying
Lu, Yan
Dong, Qing
Liu, Guihu
Chen, Feng
Zhou, Yingbing
author_sort Li, Tingchun
collection PubMed
description Chlorophylls, green pigments in chloroplasts, are essential for photosynthesis. Reduction in chlorophyll content may result in retarded growth, dwarfism, and sterility. In this study, a yellow-green leaf mutant of maize, indicative of abnormity in chlorophyll content, was identified. The physiological parameters of this mutant were measured. Next, global gene expression of this mutant was determined using transcriptome analysis and compared to that of wild-type maize plants. The yellow-green leaf mutant of maize was found to contain lower contents of chlorophyll a, chlorophyll b and carotenoid compounds. It contained fewer active PSII centers and displayed lower values of original chlorophyll fluorescence parameters than the wild-type plants. The real-time fluorescence yield, the electron transport rate, and the net photosynthetic rate of the mutant plants showed reduction as well. In contrast, the maximum photochemical quantum yield of PSII of the mutant plants was similar to that of the wild-type plants. Comparative transcriptome analysis of the mutant plants and wild-type plants led to the identification of differentially expressed 1,122 genes, of which 536 genes were up-regulated and 586 genes down-regulated in the mutant. Five genes in the chlorophyll metabolism pathway, nine genes in the tricarboxylic acid cycle and seven genes related to the conversion of sucrose to starch displayed down-regulated expression. In contrast, genes encoding a photosystem II reaction center PsbP family protein and the PGR5-like protein 1A (PGRL1A) exhibited increased transcript abundance.
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spelling pubmed-78941102021-02-23 Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize Li, Tingchun Yang, Huaying Lu, Yan Dong, Qing Liu, Guihu Chen, Feng Zhou, Yingbing PeerJ Agricultural Science Chlorophylls, green pigments in chloroplasts, are essential for photosynthesis. Reduction in chlorophyll content may result in retarded growth, dwarfism, and sterility. In this study, a yellow-green leaf mutant of maize, indicative of abnormity in chlorophyll content, was identified. The physiological parameters of this mutant were measured. Next, global gene expression of this mutant was determined using transcriptome analysis and compared to that of wild-type maize plants. The yellow-green leaf mutant of maize was found to contain lower contents of chlorophyll a, chlorophyll b and carotenoid compounds. It contained fewer active PSII centers and displayed lower values of original chlorophyll fluorescence parameters than the wild-type plants. The real-time fluorescence yield, the electron transport rate, and the net photosynthetic rate of the mutant plants showed reduction as well. In contrast, the maximum photochemical quantum yield of PSII of the mutant plants was similar to that of the wild-type plants. Comparative transcriptome analysis of the mutant plants and wild-type plants led to the identification of differentially expressed 1,122 genes, of which 536 genes were up-regulated and 586 genes down-regulated in the mutant. Five genes in the chlorophyll metabolism pathway, nine genes in the tricarboxylic acid cycle and seven genes related to the conversion of sucrose to starch displayed down-regulated expression. In contrast, genes encoding a photosystem II reaction center PsbP family protein and the PGR5-like protein 1A (PGRL1A) exhibited increased transcript abundance. PeerJ Inc. 2021-02-16 /pmc/articles/PMC7894110/ /pubmed/33628629 http://dx.doi.org/10.7717/peerj.10567 Text en © 2021 Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Li, Tingchun
Yang, Huaying
Lu, Yan
Dong, Qing
Liu, Guihu
Chen, Feng
Zhou, Yingbing
Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize
title Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize
title_full Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize
title_fullStr Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize
title_full_unstemmed Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize
title_short Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize
title_sort comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894110/
https://www.ncbi.nlm.nih.gov/pubmed/33628629
http://dx.doi.org/10.7717/peerj.10567
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