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Challenges of Using Expansion Microscopy for Super‐resolved Imaging of Cellular Organelles

Expansion microscopy (ExM) has been successfully used to improve the spatial resolution when imaging tissues by optical microscopy. In ExM, proteins of a fixed sample are crosslinked to a swellable acrylamide gel, which expands when incubated in water. Therefore, ExM allows enlarged subcellular stru...

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Detalles Bibliográficos
Autores principales: Büttner, Maximilian, Lagerholm, Christoffer B., Waithe, Dominic, Galiani, Silvia, Schliebs, Wolfgang, Erdmann, Ralf, Eggeling, Christian, Reglinski, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894168/
https://www.ncbi.nlm.nih.gov/pubmed/33049107
http://dx.doi.org/10.1002/cbic.202000571