Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells

Lactoferrin is part of the innate immune system, with antiviral activity against numerous DNA and RNA viruses. Rhinoviruses, the leading cause of the common cold, are associated with exacerbation of respiratory illnesses such as asthma. Here, we explored the effect of bovine lactoferrin (BLf) on RV-...

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Autores principales: Denani, Caio Bidueira, Real-Hohn, Antonio, de Carvalho, Carlos Alberto Marques, Gomes, Andre Marco de Oliveira, Gonçalves, Rafael Braga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894240/
https://www.ncbi.nlm.nih.gov/pubmed/33606112
http://dx.doi.org/10.1007/s00705-021-04993-4
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author Denani, Caio Bidueira
Real-Hohn, Antonio
de Carvalho, Carlos Alberto Marques
Gomes, Andre Marco de Oliveira
Gonçalves, Rafael Braga
author_facet Denani, Caio Bidueira
Real-Hohn, Antonio
de Carvalho, Carlos Alberto Marques
Gomes, Andre Marco de Oliveira
Gonçalves, Rafael Braga
author_sort Denani, Caio Bidueira
collection PubMed
description Lactoferrin is part of the innate immune system, with antiviral activity against numerous DNA and RNA viruses. Rhinoviruses, the leading cause of the common cold, are associated with exacerbation of respiratory illnesses such as asthma. Here, we explored the effect of bovine lactoferrin (BLf) on RV-B14 infectivity. Using different assays, we show that the effect of BLf is strongest during adhesion of the virus to the cell and entry. Tracking the internalisation of BLf and virus revealed a degree of colocalisation, although their interaction was only confirmed in vitro using empty viral particles, indicating a possible additional influence of BLf on other infection steps. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-04993-4.
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spelling pubmed-78942402021-02-22 Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells Denani, Caio Bidueira Real-Hohn, Antonio de Carvalho, Carlos Alberto Marques Gomes, Andre Marco de Oliveira Gonçalves, Rafael Braga Arch Virol Brief Report Lactoferrin is part of the innate immune system, with antiviral activity against numerous DNA and RNA viruses. Rhinoviruses, the leading cause of the common cold, are associated with exacerbation of respiratory illnesses such as asthma. Here, we explored the effect of bovine lactoferrin (BLf) on RV-B14 infectivity. Using different assays, we show that the effect of BLf is strongest during adhesion of the virus to the cell and entry. Tracking the internalisation of BLf and virus revealed a degree of colocalisation, although their interaction was only confirmed in vitro using empty viral particles, indicating a possible additional influence of BLf on other infection steps. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-04993-4. Springer Vienna 2021-02-19 2021 /pmc/articles/PMC7894240/ /pubmed/33606112 http://dx.doi.org/10.1007/s00705-021-04993-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Report
Denani, Caio Bidueira
Real-Hohn, Antonio
de Carvalho, Carlos Alberto Marques
Gomes, Andre Marco de Oliveira
Gonçalves, Rafael Braga
Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells
title Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells
title_full Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells
title_fullStr Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells
title_full_unstemmed Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells
title_short Lactoferrin affects rhinovirus B-14 entry into H1-HeLa cells
title_sort lactoferrin affects rhinovirus b-14 entry into h1-hela cells
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894240/
https://www.ncbi.nlm.nih.gov/pubmed/33606112
http://dx.doi.org/10.1007/s00705-021-04993-4
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