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Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus
African swine fever virus (ASFV) gives rise to a grievous transboundary and infectious disease, African swine fever (ASF), which has caused a great economic loss in the swine industry. To prevent and control ASF, once suspicious symptoms have presented, the movement of animal and pork products shoul...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894257/ https://www.ncbi.nlm.nih.gov/pubmed/33614757 http://dx.doi.org/10.3389/fvets.2020.621840 |
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author | Jia, Rui Zhang, Gaiping Liu, Hongliang Chen, Yumei Zhou, Jingming Liu, Yankai Ding, Peiyang Wang, Yanwei Zang, Weimin Wang, Aiping |
author_facet | Jia, Rui Zhang, Gaiping Liu, Hongliang Chen, Yumei Zhou, Jingming Liu, Yankai Ding, Peiyang Wang, Yanwei Zang, Weimin Wang, Aiping |
author_sort | Jia, Rui |
collection | PubMed |
description | African swine fever virus (ASFV) gives rise to a grievous transboundary and infectious disease, African swine fever (ASF), which has caused a great economic loss in the swine industry. To prevent and control ASF, once suspicious symptoms have presented, the movement of animal and pork products should be stopped, and then, laboratory testing should be adopted to diagnose ASF. A method for ASFV DNA quantification is presented in this research, which utilizes the next-generation PCR platform, nanofluidic chip digital PCR (cdPCR). The cdPCR detection showed good linearity and repeatability. The limit of detection for cdPCR is 30.1995 copies per reaction, whereas no non-specific amplification curve was found with other swine viruses. In the detection of 69 clinical samples, the cdPCR showed significant consistency [91.30% (63/69)] to the Office International des Epizooties-approved quantitative PCR. Compared with the commercial quantitative PCR kit, the sensitivity of the cdPCR assay was 86.27% (44/50), and the specificity was 94.44% (17/18). The positive coincidence rate of the cdPCR assay was 88% (44/50). The total coincidence rate of the cdPCR and kit was 89.86% (62/69), and the kappa value reached 0.800 (P < 0.0001). This is the first time that cdPCR has been applied to detecting ASFV successfully. |
format | Online Article Text |
id | pubmed-7894257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78942572021-02-20 Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus Jia, Rui Zhang, Gaiping Liu, Hongliang Chen, Yumei Zhou, Jingming Liu, Yankai Ding, Peiyang Wang, Yanwei Zang, Weimin Wang, Aiping Front Vet Sci Veterinary Science African swine fever virus (ASFV) gives rise to a grievous transboundary and infectious disease, African swine fever (ASF), which has caused a great economic loss in the swine industry. To prevent and control ASF, once suspicious symptoms have presented, the movement of animal and pork products should be stopped, and then, laboratory testing should be adopted to diagnose ASF. A method for ASFV DNA quantification is presented in this research, which utilizes the next-generation PCR platform, nanofluidic chip digital PCR (cdPCR). The cdPCR detection showed good linearity and repeatability. The limit of detection for cdPCR is 30.1995 copies per reaction, whereas no non-specific amplification curve was found with other swine viruses. In the detection of 69 clinical samples, the cdPCR showed significant consistency [91.30% (63/69)] to the Office International des Epizooties-approved quantitative PCR. Compared with the commercial quantitative PCR kit, the sensitivity of the cdPCR assay was 86.27% (44/50), and the specificity was 94.44% (17/18). The positive coincidence rate of the cdPCR assay was 88% (44/50). The total coincidence rate of the cdPCR and kit was 89.86% (62/69), and the kappa value reached 0.800 (P < 0.0001). This is the first time that cdPCR has been applied to detecting ASFV successfully. Frontiers Media S.A. 2021-02-05 /pmc/articles/PMC7894257/ /pubmed/33614757 http://dx.doi.org/10.3389/fvets.2020.621840 Text en Copyright © 2021 Jia, Zhang, Liu, Chen, Zhou, Liu, Ding, Wang, Zang and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Jia, Rui Zhang, Gaiping Liu, Hongliang Chen, Yumei Zhou, Jingming Liu, Yankai Ding, Peiyang Wang, Yanwei Zang, Weimin Wang, Aiping Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus |
title | Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus |
title_full | Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus |
title_fullStr | Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus |
title_full_unstemmed | Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus |
title_short | Novel Application of Nanofluidic Chip Digital PCR for Detection of African Swine Fever Virus |
title_sort | novel application of nanofluidic chip digital pcr for detection of african swine fever virus |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894257/ https://www.ncbi.nlm.nih.gov/pubmed/33614757 http://dx.doi.org/10.3389/fvets.2020.621840 |
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