STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system

Amyloids are the fibrillar protein aggregates with cross-β structure. Traditionally amyloids were associated with pathology, however, nowadays more data is emerging about functional amyloids playing essential roles in cellular processes. We conducted screening for functional amyloids in rat brain. O...

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Autores principales: Chirinskaite, A.V., Siniukova, V.A., Velizhanina, M.E., Sopova, J.V., Belashova, T.A., Zadorsky, S.P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Short Communicatios
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894455/
https://www.ncbi.nlm.nih.gov/pubmed/33590815
http://dx.doi.org/10.1080/19336896.2021.1883980
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author Chirinskaite, A.V.
Siniukova, V.A.
Velizhanina, M.E.
Sopova, J.V.
Belashova, T.A.
Zadorsky, S.P.
author_facet Chirinskaite, A.V.
Siniukova, V.A.
Velizhanina, M.E.
Sopova, J.V.
Belashova, T.A.
Zadorsky, S.P.
author_sort Chirinskaite, A.V.
collection PubMed
description Amyloids are the fibrillar protein aggregates with cross-β structure. Traditionally amyloids were associated with pathology, however, nowadays more data is emerging about functional amyloids playing essential roles in cellular processes. We conducted screening for functional amyloids in rat brain. One of the identified proteins was STXBP1 taking part in vesicular transport and neurotransmitter secretion. Using SDD-AGE and protein fractionation we found out that STXBP1 forms small detergent-insoluble aggregates in rat brain. With immunoprecipitation analysis and C-DAG system, we showed that STXBP1 forms amyloid-like fibrils. Thus, STXBP1 demonstrates amyloid properties in rat brain and in bacterial expression system.
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spelling pubmed-78944552021-02-26 STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system Chirinskaite, A.V. Siniukova, V.A. Velizhanina, M.E. Sopova, J.V. Belashova, T.A. Zadorsky, S.P. Prion Short Communicatios Amyloids are the fibrillar protein aggregates with cross-β structure. Traditionally amyloids were associated with pathology, however, nowadays more data is emerging about functional amyloids playing essential roles in cellular processes. We conducted screening for functional amyloids in rat brain. One of the identified proteins was STXBP1 taking part in vesicular transport and neurotransmitter secretion. Using SDD-AGE and protein fractionation we found out that STXBP1 forms small detergent-insoluble aggregates in rat brain. With immunoprecipitation analysis and C-DAG system, we showed that STXBP1 forms amyloid-like fibrils. Thus, STXBP1 demonstrates amyloid properties in rat brain and in bacterial expression system. Taylor & Francis 2021-02-16 /pmc/articles/PMC7894455/ /pubmed/33590815 http://dx.doi.org/10.1080/19336896.2021.1883980 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communicatios
Chirinskaite, A.V.
Siniukova, V.A.
Velizhanina, M.E.
Sopova, J.V.
Belashova, T.A.
Zadorsky, S.P.
STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system
title STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system
title_full STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system
title_fullStr STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system
title_full_unstemmed STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system
title_short STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system
title_sort stxbp1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system
topic Short Communicatios
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894455/
https://www.ncbi.nlm.nih.gov/pubmed/33590815
http://dx.doi.org/10.1080/19336896.2021.1883980
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