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Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress

Heat‐stressed Arabidopsis plants release heterochromatin‐associated transposable element (TE) silencing, yet it is not accompanied by major reductions of epigenetic repressive modifications. In this study, we explored the functional role of histone H1 in repressing heterochromatic TEs in response to...

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Autores principales: Liu, Shujing, de Jonge, Jennifer, Trejo‐Arellano, Minerva S., Santos‐González, Juan, Köhler, Claudia, Hennig, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894476/
https://www.ncbi.nlm.nih.gov/pubmed/33091182
http://dx.doi.org/10.1111/nph.17018
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author Liu, Shujing
de Jonge, Jennifer
Trejo‐Arellano, Minerva S.
Santos‐González, Juan
Köhler, Claudia
Hennig, Lars
author_facet Liu, Shujing
de Jonge, Jennifer
Trejo‐Arellano, Minerva S.
Santos‐González, Juan
Köhler, Claudia
Hennig, Lars
author_sort Liu, Shujing
collection PubMed
description Heat‐stressed Arabidopsis plants release heterochromatin‐associated transposable element (TE) silencing, yet it is not accompanied by major reductions of epigenetic repressive modifications. In this study, we explored the functional role of histone H1 in repressing heterochromatic TEs in response to heat stress. We generated and analyzed RNA and bisulfite‐sequencing data of wild‐type and h1 mutant seedlings before and after heat stress. Loss of H1 caused activation of pericentromeric Gypsy elements upon heat treatment, despite these elements remaining highly methylated. By contrast, nonpericentromeric Copia elements became activated concomitantly with loss of DNA methylation. The same Copia elements became activated in heat‐treated chromomethylase 2 (cmt2) mutants, indicating that H1 represses Copia elements through maintaining DNA methylation under heat. We discovered that H1 is required for TE repression in response to heat stress, but its functional role differs depending on TE location. Strikingly, H1‐deficient plants treated with the DNA methyltransferase inhibitor zebularine were highly tolerant to heat stress, suggesting that both H1 and DNA methylation redundantly suppress the plant response to heat stress.
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spelling pubmed-78944762021-03-02 Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress Liu, Shujing de Jonge, Jennifer Trejo‐Arellano, Minerva S. Santos‐González, Juan Köhler, Claudia Hennig, Lars New Phytol Research Heat‐stressed Arabidopsis plants release heterochromatin‐associated transposable element (TE) silencing, yet it is not accompanied by major reductions of epigenetic repressive modifications. In this study, we explored the functional role of histone H1 in repressing heterochromatic TEs in response to heat stress. We generated and analyzed RNA and bisulfite‐sequencing data of wild‐type and h1 mutant seedlings before and after heat stress. Loss of H1 caused activation of pericentromeric Gypsy elements upon heat treatment, despite these elements remaining highly methylated. By contrast, nonpericentromeric Copia elements became activated concomitantly with loss of DNA methylation. The same Copia elements became activated in heat‐treated chromomethylase 2 (cmt2) mutants, indicating that H1 represses Copia elements through maintaining DNA methylation under heat. We discovered that H1 is required for TE repression in response to heat stress, but its functional role differs depending on TE location. Strikingly, H1‐deficient plants treated with the DNA methyltransferase inhibitor zebularine were highly tolerant to heat stress, suggesting that both H1 and DNA methylation redundantly suppress the plant response to heat stress. John Wiley and Sons Inc. 2020-11-22 2021-02 /pmc/articles/PMC7894476/ /pubmed/33091182 http://dx.doi.org/10.1111/nph.17018 Text en © 2020 Swedish University of Agricultural Sciences. New Phytologist © 2020 New Phytologist Foundation This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research
Liu, Shujing
de Jonge, Jennifer
Trejo‐Arellano, Minerva S.
Santos‐González, Juan
Köhler, Claudia
Hennig, Lars
Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress
title Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress
title_full Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress
title_fullStr Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress
title_full_unstemmed Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress
title_short Role of H1 and DNA methylation in selective regulation of transposable elements during heat stress
title_sort role of h1 and dna methylation in selective regulation of transposable elements during heat stress
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894476/
https://www.ncbi.nlm.nih.gov/pubmed/33091182
http://dx.doi.org/10.1111/nph.17018
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