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The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway

In the adult hypothalamus, the neuronal precursor role is attributed to the radial glia‐like cells that line the third‐ventricle (3V) wall called tanycytes. Under nutritional cues, including hypercaloric diets, tanycytes proliferate and differentiate into mature neurons that moderate body weight, su...

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Autores principales: Recabal, Antonia, Fernández, Paola, López, Sergio, Barahona, María J., Ordenes, Patricio, Palma, Alejandra, Elizondo‐Vega, Roberto, Farkas, Carlos, Uribe, Amparo, Caprile, Teresa, Sáez, Juan C., García‐Robles, María A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894481/
https://www.ncbi.nlm.nih.gov/pubmed/32936929
http://dx.doi.org/10.1111/jnc.15188
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author Recabal, Antonia
Fernández, Paola
López, Sergio
Barahona, María J.
Ordenes, Patricio
Palma, Alejandra
Elizondo‐Vega, Roberto
Farkas, Carlos
Uribe, Amparo
Caprile, Teresa
Sáez, Juan C.
García‐Robles, María A.
author_facet Recabal, Antonia
Fernández, Paola
López, Sergio
Barahona, María J.
Ordenes, Patricio
Palma, Alejandra
Elizondo‐Vega, Roberto
Farkas, Carlos
Uribe, Amparo
Caprile, Teresa
Sáez, Juan C.
García‐Robles, María A.
author_sort Recabal, Antonia
collection PubMed
description In the adult hypothalamus, the neuronal precursor role is attributed to the radial glia‐like cells that line the third‐ventricle (3V) wall called tanycytes. Under nutritional cues, including hypercaloric diets, tanycytes proliferate and differentiate into mature neurons that moderate body weight, suggesting that hypothalamic neurogenesis is an adaptive mechanism in response to metabolic changes. Previous studies have shown that the tanycyte glucosensing mechanism depends on connexin‐43 hemichannels (Cx43 HCs), purine release, and increased intracellular free calcium ion concentration [(Ca(2+))(i)] mediated by purinergic P2Y receptors. Since, Fibroblast Growth Factor 2 (FGF2) causes similar purinergic events in other cell types, we hypothesize that this pathway can be also activated by FGF2 in tanycytes to promote their proliferation. Here, we used bromodeoxyuridine (BrdU) incorporation to evaluate if FGF2‐induced tanycyte cell division is sensitive to Cx43 HC inhibition in vitro and in vivo. Immunocytochemical analyses showed that cultured tanycytes maintain the expression of in situ markers. After FGF2 exposure, tanycytic Cx43 HCs opened, enabling release of ATP to the extracellular milieu. Moreover, application of external ATP was enough to induce their cell division, which could be suppressed by Cx43 HC or P2Y1‐receptor inhibitors. Similarly, in vivo experiments performed on rats by continuous infusion of FGF2 and a Cx43 HC inhibitor into the 3V, demonstrated that FGF2‐induced β‐tanycyte proliferation is sensitive to Cx43 HC blockade. Thus, FGF2 induced Cx43 HC opening, triggered purinergic signaling, and increased β‐tanycytes proliferation, highlighting some of the molecular mechanisms involved in the cell division response of tanycyte. [Image: see text] This article has an Editorial Highlight see https://doi.org/10.1111/jnc.15218.
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spelling pubmed-78944812021-03-02 The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway Recabal, Antonia Fernández, Paola López, Sergio Barahona, María J. Ordenes, Patricio Palma, Alejandra Elizondo‐Vega, Roberto Farkas, Carlos Uribe, Amparo Caprile, Teresa Sáez, Juan C. García‐Robles, María A. J Neurochem ORIGINAL ARTICLES In the adult hypothalamus, the neuronal precursor role is attributed to the radial glia‐like cells that line the third‐ventricle (3V) wall called tanycytes. Under nutritional cues, including hypercaloric diets, tanycytes proliferate and differentiate into mature neurons that moderate body weight, suggesting that hypothalamic neurogenesis is an adaptive mechanism in response to metabolic changes. Previous studies have shown that the tanycyte glucosensing mechanism depends on connexin‐43 hemichannels (Cx43 HCs), purine release, and increased intracellular free calcium ion concentration [(Ca(2+))(i)] mediated by purinergic P2Y receptors. Since, Fibroblast Growth Factor 2 (FGF2) causes similar purinergic events in other cell types, we hypothesize that this pathway can be also activated by FGF2 in tanycytes to promote their proliferation. Here, we used bromodeoxyuridine (BrdU) incorporation to evaluate if FGF2‐induced tanycyte cell division is sensitive to Cx43 HC inhibition in vitro and in vivo. Immunocytochemical analyses showed that cultured tanycytes maintain the expression of in situ markers. After FGF2 exposure, tanycytic Cx43 HCs opened, enabling release of ATP to the extracellular milieu. Moreover, application of external ATP was enough to induce their cell division, which could be suppressed by Cx43 HC or P2Y1‐receptor inhibitors. Similarly, in vivo experiments performed on rats by continuous infusion of FGF2 and a Cx43 HC inhibitor into the 3V, demonstrated that FGF2‐induced β‐tanycyte proliferation is sensitive to Cx43 HC blockade. Thus, FGF2 induced Cx43 HC opening, triggered purinergic signaling, and increased β‐tanycytes proliferation, highlighting some of the molecular mechanisms involved in the cell division response of tanycyte. [Image: see text] This article has an Editorial Highlight see https://doi.org/10.1111/jnc.15218. John Wiley and Sons Inc. 2020-10-19 2021-01 /pmc/articles/PMC7894481/ /pubmed/32936929 http://dx.doi.org/10.1111/jnc.15188 Text en © 2020 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle ORIGINAL ARTICLES
Recabal, Antonia
Fernández, Paola
López, Sergio
Barahona, María J.
Ordenes, Patricio
Palma, Alejandra
Elizondo‐Vega, Roberto
Farkas, Carlos
Uribe, Amparo
Caprile, Teresa
Sáez, Juan C.
García‐Robles, María A.
The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway
title The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway
title_full The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway
title_fullStr The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway
title_full_unstemmed The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway
title_short The FGF2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway
title_sort fgf2‐induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic‐dependent pathway
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894481/
https://www.ncbi.nlm.nih.gov/pubmed/32936929
http://dx.doi.org/10.1111/jnc.15188
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