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Antimalarial Activity of Seed Extracts of Schinus molle Against Plasmodium berghei in Mice

BACKGROUND. Malaria is a major public health problem in developing countries. In Ethiopian, the seeds of Schinus molle are used for the management of malaria. Therefore, the current study aimed to evaluate in vivo antimalarial activity of hydro-alcoholic crude extract and solvent fractions of Schinu...

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Autores principales: Mekuria, Abebe Basazn, Geta, Mestayet, Birru, Eshetie Melese, Gelayee, Desalegn Asmelashe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894695/
https://www.ncbi.nlm.nih.gov/pubmed/33593094
http://dx.doi.org/10.1177/2515690X20984287
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author Mekuria, Abebe Basazn
Geta, Mestayet
Birru, Eshetie Melese
Gelayee, Desalegn Asmelashe
author_facet Mekuria, Abebe Basazn
Geta, Mestayet
Birru, Eshetie Melese
Gelayee, Desalegn Asmelashe
author_sort Mekuria, Abebe Basazn
collection PubMed
description BACKGROUND. Malaria is a major public health problem in developing countries. In Ethiopian, the seeds of Schinus molle are used for the management of malaria. Therefore, the current study aimed to evaluate in vivo antimalarial activity of hydro-alcoholic crude extract and solvent fractions of Schinus molle seeds in Plasmodium berghei infected mice. METHODS. An 80 percent of methanolic crude extract and solvent fractions of Schinus molle seeds were tested for antimalarial activity at 100, 200 and 400 mg/kg doses. The parasitemia level, packed cell volume, body weight, survival of date and rectal temperature were used to evaluate the anti-malarial activity of the extracts. One-way ANOVA followed by post hoc Tukey’s HSD multiple comparison test was employed and the result was expressed in mean ± SEM (standard error of the mean). RESULTS. The curative activity of the highest dose of crude extract and aqueous fraction of Schinus molle seeds was 69.86% and 73.82% (p < 0.001), respectively. In the prophylactic test, aqueous fraction had 72.39% (p < 0.001) suppression antimalarial activity. Among solvent fractions, only chloroform fraction was significantly attenuated packed cell volume reduction. The mice treated with crude extract and aqueous fraction had longer survival date than vehicle-treated mice (p < 0.001). CONCLUSION. The experiment finding showed that the crude extract and solvent fractions of Schinus molle seeds had significant curative and prophylaxis anti-plasmodial activity. This result revealed that the Schinus molle seeds extract has promising antimalarial activity against Plasmodium berghei. However, further confirmatory studies, isolation and characterization of the active constituents are recommended.
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spelling pubmed-78946952021-02-26 Antimalarial Activity of Seed Extracts of Schinus molle Against Plasmodium berghei in Mice Mekuria, Abebe Basazn Geta, Mestayet Birru, Eshetie Melese Gelayee, Desalegn Asmelashe J Evid Based Integr Med Original Article BACKGROUND. Malaria is a major public health problem in developing countries. In Ethiopian, the seeds of Schinus molle are used for the management of malaria. Therefore, the current study aimed to evaluate in vivo antimalarial activity of hydro-alcoholic crude extract and solvent fractions of Schinus molle seeds in Plasmodium berghei infected mice. METHODS. An 80 percent of methanolic crude extract and solvent fractions of Schinus molle seeds were tested for antimalarial activity at 100, 200 and 400 mg/kg doses. The parasitemia level, packed cell volume, body weight, survival of date and rectal temperature were used to evaluate the anti-malarial activity of the extracts. One-way ANOVA followed by post hoc Tukey’s HSD multiple comparison test was employed and the result was expressed in mean ± SEM (standard error of the mean). RESULTS. The curative activity of the highest dose of crude extract and aqueous fraction of Schinus molle seeds was 69.86% and 73.82% (p < 0.001), respectively. In the prophylactic test, aqueous fraction had 72.39% (p < 0.001) suppression antimalarial activity. Among solvent fractions, only chloroform fraction was significantly attenuated packed cell volume reduction. The mice treated with crude extract and aqueous fraction had longer survival date than vehicle-treated mice (p < 0.001). CONCLUSION. The experiment finding showed that the crude extract and solvent fractions of Schinus molle seeds had significant curative and prophylaxis anti-plasmodial activity. This result revealed that the Schinus molle seeds extract has promising antimalarial activity against Plasmodium berghei. However, further confirmatory studies, isolation and characterization of the active constituents are recommended. SAGE Publications 2021-02-16 /pmc/articles/PMC7894695/ /pubmed/33593094 http://dx.doi.org/10.1177/2515690X20984287 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Mekuria, Abebe Basazn
Geta, Mestayet
Birru, Eshetie Melese
Gelayee, Desalegn Asmelashe
Antimalarial Activity of Seed Extracts of Schinus molle Against Plasmodium berghei in Mice
title Antimalarial Activity of Seed Extracts of Schinus molle Against Plasmodium berghei in Mice
title_full Antimalarial Activity of Seed Extracts of Schinus molle Against Plasmodium berghei in Mice
title_fullStr Antimalarial Activity of Seed Extracts of Schinus molle Against Plasmodium berghei in Mice
title_full_unstemmed Antimalarial Activity of Seed Extracts of Schinus molle Against Plasmodium berghei in Mice
title_short Antimalarial Activity of Seed Extracts of Schinus molle Against Plasmodium berghei in Mice
title_sort antimalarial activity of seed extracts of schinus molle against plasmodium berghei in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894695/
https://www.ncbi.nlm.nih.gov/pubmed/33593094
http://dx.doi.org/10.1177/2515690X20984287
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