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Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain

Amino acids are delivered into developing wheat grains to support the accumulation of storage proteins in the starchy endosperm, and transporters play important roles in regulating this process. RNA-seq, RT-qPCR, and promoter-GUS assays showed that three amino acid transporters are differentially ex...

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Autores principales: Wan, Yongfang, Wang, Yan, Shi, Zhiqiang, Rentsch, Doris, Ward, Jane L., Hassall, Kirsty, Sparks, Caroline A., Huttly, Alison K., Buchner, Peter, Powers, Stephen, Shewry, Peter R., Hawkesford, Malcolm J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894817/
https://www.ncbi.nlm.nih.gov/pubmed/33606697
http://dx.doi.org/10.1371/journal.pone.0246763
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author Wan, Yongfang
Wang, Yan
Shi, Zhiqiang
Rentsch, Doris
Ward, Jane L.
Hassall, Kirsty
Sparks, Caroline A.
Huttly, Alison K.
Buchner, Peter
Powers, Stephen
Shewry, Peter R.
Hawkesford, Malcolm J.
author_facet Wan, Yongfang
Wang, Yan
Shi, Zhiqiang
Rentsch, Doris
Ward, Jane L.
Hassall, Kirsty
Sparks, Caroline A.
Huttly, Alison K.
Buchner, Peter
Powers, Stephen
Shewry, Peter R.
Hawkesford, Malcolm J.
author_sort Wan, Yongfang
collection PubMed
description Amino acids are delivered into developing wheat grains to support the accumulation of storage proteins in the starchy endosperm, and transporters play important roles in regulating this process. RNA-seq, RT-qPCR, and promoter-GUS assays showed that three amino acid transporters are differentially expressed in the endosperm transfer cells (TaAAP2), starchy endosperm cells (TaAAP13), and aleurone cells and embryo of the developing grain (TaAAP21), respectively. Yeast complementation revealed that all three transporters can transport a broad spectrum of amino acids. RNAi-mediated suppression of TaAAP13 expression in the starchy endosperm did not reduce the total nitrogen content of the whole grain, but significantly altered the composition and distribution of metabolites in the starchy endosperm, with increasing concentrations of some amino acids (notably glutamine and glycine) from the outer to inner starchy endosperm cells compared with wild type. Overexpression of TaAAP13 under the endosperm-specific HMW-GS (high molecular weight glutenin subunit) promoter significantly increased grain size, grain nitrogen concentration, and thousand grain weight, indicating that the sink strength for nitrogen transport was increased by manipulation of amino acid transporters. However, the total grain number was reduced, suggesting that source nitrogen remobilized from leaves is a limiting factor for productivity. Therefore, simultaneously increasing loading of amino acids into the phloem and delivery to the spike would be required to increase protein content while maintaining grain yield.
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spelling pubmed-78948172021-03-01 Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain Wan, Yongfang Wang, Yan Shi, Zhiqiang Rentsch, Doris Ward, Jane L. Hassall, Kirsty Sparks, Caroline A. Huttly, Alison K. Buchner, Peter Powers, Stephen Shewry, Peter R. Hawkesford, Malcolm J. PLoS One Research Article Amino acids are delivered into developing wheat grains to support the accumulation of storage proteins in the starchy endosperm, and transporters play important roles in regulating this process. RNA-seq, RT-qPCR, and promoter-GUS assays showed that three amino acid transporters are differentially expressed in the endosperm transfer cells (TaAAP2), starchy endosperm cells (TaAAP13), and aleurone cells and embryo of the developing grain (TaAAP21), respectively. Yeast complementation revealed that all three transporters can transport a broad spectrum of amino acids. RNAi-mediated suppression of TaAAP13 expression in the starchy endosperm did not reduce the total nitrogen content of the whole grain, but significantly altered the composition and distribution of metabolites in the starchy endosperm, with increasing concentrations of some amino acids (notably glutamine and glycine) from the outer to inner starchy endosperm cells compared with wild type. Overexpression of TaAAP13 under the endosperm-specific HMW-GS (high molecular weight glutenin subunit) promoter significantly increased grain size, grain nitrogen concentration, and thousand grain weight, indicating that the sink strength for nitrogen transport was increased by manipulation of amino acid transporters. However, the total grain number was reduced, suggesting that source nitrogen remobilized from leaves is a limiting factor for productivity. Therefore, simultaneously increasing loading of amino acids into the phloem and delivery to the spike would be required to increase protein content while maintaining grain yield. Public Library of Science 2021-02-19 /pmc/articles/PMC7894817/ /pubmed/33606697 http://dx.doi.org/10.1371/journal.pone.0246763 Text en © 2021 Wan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wan, Yongfang
Wang, Yan
Shi, Zhiqiang
Rentsch, Doris
Ward, Jane L.
Hassall, Kirsty
Sparks, Caroline A.
Huttly, Alison K.
Buchner, Peter
Powers, Stephen
Shewry, Peter R.
Hawkesford, Malcolm J.
Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
title Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
title_full Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
title_fullStr Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
title_full_unstemmed Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
title_short Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
title_sort wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894817/
https://www.ncbi.nlm.nih.gov/pubmed/33606697
http://dx.doi.org/10.1371/journal.pone.0246763
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