Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae

Lumpy skin disease (LSD) is an emerging disease of cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, retention of Lumpy Skin Disease Virus (LSDV) by three Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. A vi...

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Autores principales: Issimov, Arman, Taylor, David B., Shalmenov, Malik, Nurgaliyev, Birzhan, Zhubantayev, Izimgali, Abekeshev, Nurzhan, Kushaliyev, Kaissar, Kereyev, Abzal, Kutumbetov, Lespek, Zhanabayev, Assylbek, Zhakiyanova, Yasmin, White, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894917/
https://www.ncbi.nlm.nih.gov/pubmed/33606690
http://dx.doi.org/10.1371/journal.pone.0238210
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author Issimov, Arman
Taylor, David B.
Shalmenov, Malik
Nurgaliyev, Birzhan
Zhubantayev, Izimgali
Abekeshev, Nurzhan
Kushaliyev, Kaissar
Kereyev, Abzal
Kutumbetov, Lespek
Zhanabayev, Assylbek
Zhakiyanova, Yasmin
White, Peter J.
author_facet Issimov, Arman
Taylor, David B.
Shalmenov, Malik
Nurgaliyev, Birzhan
Zhubantayev, Izimgali
Abekeshev, Nurzhan
Kushaliyev, Kaissar
Kereyev, Abzal
Kutumbetov, Lespek
Zhanabayev, Assylbek
Zhakiyanova, Yasmin
White, Peter J.
author_sort Issimov, Arman
collection PubMed
description Lumpy skin disease (LSD) is an emerging disease of cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, retention of Lumpy Skin Disease Virus (LSDV) by three Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. A virulent LSDV strain was injected into the thorax of flies to bypass the midgut barrier. The fate of the pathogen in the hemolymph of the flies was examined using PCR and virus isolation tests. LSDV was isolated from all three Stomoxys species up to 24h post inoculation while virus DNA was detectable up to 7d post inoculation.
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spelling pubmed-78949172021-03-01 Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae Issimov, Arman Taylor, David B. Shalmenov, Malik Nurgaliyev, Birzhan Zhubantayev, Izimgali Abekeshev, Nurzhan Kushaliyev, Kaissar Kereyev, Abzal Kutumbetov, Lespek Zhanabayev, Assylbek Zhakiyanova, Yasmin White, Peter J. PLoS One Research Article Lumpy skin disease (LSD) is an emerging disease of cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, retention of Lumpy Skin Disease Virus (LSDV) by three Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. A virulent LSDV strain was injected into the thorax of flies to bypass the midgut barrier. The fate of the pathogen in the hemolymph of the flies was examined using PCR and virus isolation tests. LSDV was isolated from all three Stomoxys species up to 24h post inoculation while virus DNA was detectable up to 7d post inoculation. Public Library of Science 2021-02-19 /pmc/articles/PMC7894917/ /pubmed/33606690 http://dx.doi.org/10.1371/journal.pone.0238210 Text en © 2021 Issimov et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Issimov, Arman
Taylor, David B.
Shalmenov, Malik
Nurgaliyev, Birzhan
Zhubantayev, Izimgali
Abekeshev, Nurzhan
Kushaliyev, Kaissar
Kereyev, Abzal
Kutumbetov, Lespek
Zhanabayev, Assylbek
Zhakiyanova, Yasmin
White, Peter J.
Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae
title Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae
title_full Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae
title_fullStr Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae
title_full_unstemmed Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae
title_short Retention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae
title_sort retention of lumpy skin disease virus in stomoxys spp (stomoxys calcitrans, stomoxys sitiens, stomoxys indica) following intrathoracic inoculation, diptera: muscidae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894917/
https://www.ncbi.nlm.nih.gov/pubmed/33606690
http://dx.doi.org/10.1371/journal.pone.0238210
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