Cargando…

Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data

Colorectal cancer is the 2nd leading cause of cancer-related deaths in the world. The mechanisms underlying CRC development, progression, and resistance to treatment are complex and not fully understood. The immune response in the tumor microenvironment has been shown to play a significant role in m...

Descripción completa

Detalles Bibliográficos
Autores principales: Saunders, Apryl S., Bender, Dawn E., Ray, Anita L., Wu, Xiangyan, Morris, Katherine T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895368/
https://www.ncbi.nlm.nih.gov/pubmed/33606788
http://dx.doi.org/10.1371/journal.pone.0247233
_version_ 1783653349703286784
author Saunders, Apryl S.
Bender, Dawn E.
Ray, Anita L.
Wu, Xiangyan
Morris, Katherine T.
author_facet Saunders, Apryl S.
Bender, Dawn E.
Ray, Anita L.
Wu, Xiangyan
Morris, Katherine T.
author_sort Saunders, Apryl S.
collection PubMed
description Colorectal cancer is the 2nd leading cause of cancer-related deaths in the world. The mechanisms underlying CRC development, progression, and resistance to treatment are complex and not fully understood. The immune response in the tumor microenvironment has been shown to play a significant role in many cancers, including colorectal cancer. Colony-stimulating factor 3 (CSF3) has been associated with changes to the immune environment in colorectal cancer animal models. We hypothesized that CSF3 signaling would correlate with pro-tumor tumor microenvironment changes associated with immune infiltrate and response. We utilized publicly available datasets to guide future mechanistic studies of the role CSF3 and its receptor (CSF3R) play in colorectal cancer development and progression. Here, we use bioinformatics data and mRNA from patients with colon (n = 242) or rectal (n = 92) cancers, obtained from The Cancer Genome Atlas Firehose Legacy dataset. We examined correlations of CSF3 and CSF3R expression with patient demographics, tumor stage and consensus molecular subtype classification. Gene expression correlations, cell type enrichment, Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data scores and Gene Ontology were used to analyze expression of receptor and ligand, tumor microenvironment infiltration of immune cells, and alterations in biological pathways. We found that CSF3 and CSF3R expression is highest in consensus molecular subtype 1 and consensus molecular subtype 4. Ligand and receptor expression are also correlated with changes in T cell and macrophage signatures. CSF3R significantly correlates with a large number of genes that are associated with poor colorectal cancer prognosis.
format Online
Article
Text
id pubmed-7895368
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-78953682021-03-01 Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data Saunders, Apryl S. Bender, Dawn E. Ray, Anita L. Wu, Xiangyan Morris, Katherine T. PLoS One Research Article Colorectal cancer is the 2nd leading cause of cancer-related deaths in the world. The mechanisms underlying CRC development, progression, and resistance to treatment are complex and not fully understood. The immune response in the tumor microenvironment has been shown to play a significant role in many cancers, including colorectal cancer. Colony-stimulating factor 3 (CSF3) has been associated with changes to the immune environment in colorectal cancer animal models. We hypothesized that CSF3 signaling would correlate with pro-tumor tumor microenvironment changes associated with immune infiltrate and response. We utilized publicly available datasets to guide future mechanistic studies of the role CSF3 and its receptor (CSF3R) play in colorectal cancer development and progression. Here, we use bioinformatics data and mRNA from patients with colon (n = 242) or rectal (n = 92) cancers, obtained from The Cancer Genome Atlas Firehose Legacy dataset. We examined correlations of CSF3 and CSF3R expression with patient demographics, tumor stage and consensus molecular subtype classification. Gene expression correlations, cell type enrichment, Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data scores and Gene Ontology were used to analyze expression of receptor and ligand, tumor microenvironment infiltration of immune cells, and alterations in biological pathways. We found that CSF3 and CSF3R expression is highest in consensus molecular subtype 1 and consensus molecular subtype 4. Ligand and receptor expression are also correlated with changes in T cell and macrophage signatures. CSF3R significantly correlates with a large number of genes that are associated with poor colorectal cancer prognosis. Public Library of Science 2021-02-19 /pmc/articles/PMC7895368/ /pubmed/33606788 http://dx.doi.org/10.1371/journal.pone.0247233 Text en © 2021 Saunders et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Saunders, Apryl S.
Bender, Dawn E.
Ray, Anita L.
Wu, Xiangyan
Morris, Katherine T.
Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data
title Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data
title_full Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data
title_fullStr Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data
title_full_unstemmed Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data
title_short Colony-stimulating factor 3 signaling in colon and rectal cancers: Immune response and CMS classification in TCGA data
title_sort colony-stimulating factor 3 signaling in colon and rectal cancers: immune response and cms classification in tcga data
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895368/
https://www.ncbi.nlm.nih.gov/pubmed/33606788
http://dx.doi.org/10.1371/journal.pone.0247233
work_keys_str_mv AT saundersapryls colonystimulatingfactor3signalingincolonandrectalcancersimmuneresponseandcmsclassificationintcgadata
AT benderdawne colonystimulatingfactor3signalingincolonandrectalcancersimmuneresponseandcmsclassificationintcgadata
AT rayanital colonystimulatingfactor3signalingincolonandrectalcancersimmuneresponseandcmsclassificationintcgadata
AT wuxiangyan colonystimulatingfactor3signalingincolonandrectalcancersimmuneresponseandcmsclassificationintcgadata
AT morriskatherinet colonystimulatingfactor3signalingincolonandrectalcancersimmuneresponseandcmsclassificationintcgadata