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Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya

The bioecology of phlebotomine sand flies is intimately linked to the utilization of environmental resources including plant feeding. However, plant feeding behavior of sand flies remains largely understudied for Afrotropical species. Here, using a combination of biochemical, molecular, and chemical...

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Autores principales: Hassaballa, Iman B., Sole, Catherine L., Cheseto, Xavier, Torto, Baldwyn, Tchouassi, David P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895382/
https://www.ncbi.nlm.nih.gov/pubmed/33556068
http://dx.doi.org/10.1371/journal.pntd.0009041
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author Hassaballa, Iman B.
Sole, Catherine L.
Cheseto, Xavier
Torto, Baldwyn
Tchouassi, David P.
author_facet Hassaballa, Iman B.
Sole, Catherine L.
Cheseto, Xavier
Torto, Baldwyn
Tchouassi, David P.
author_sort Hassaballa, Iman B.
collection PubMed
description The bioecology of phlebotomine sand flies is intimately linked to the utilization of environmental resources including plant feeding. However, plant feeding behavior of sand flies remains largely understudied for Afrotropical species. Here, using a combination of biochemical, molecular, and chemical approaches, we decipher specific plant-feeding associations in field-collected sand flies from a dry ecology endemic for leishmaniasis in Kenya. Cold-anthrone test indicative of recent plant feeding showed that fructose positivity rates were similar in both sand fly sexes and between those sampled indoors and outdoors. Analysis of derived sequences of the ribulose-1,5-bisphosphate carboxylase large subunit gene (rbcL) from fructose-positive specimens implicated mainly Acacia plants in the family Fabaceae (73%) as those readily foraged on by both sexes of Phlebotomus and Sergentomyia. Chemical analysis by high performance liquid chromatography detected fructose as the most common sugar in sand flies and leaves of selected plant species in the Fabaceae family. Analysis of similarities (ANOSIM) of the headspace volatile profiles of selected Fabaceae plants identified benzyl alcohol, (Z)-linalool oxide, (E)-β-ocimene, p-cymene, p-cresol, and m-cresol, as discriminating compounds between the plant volatiles. These results indicate selective sand fly plant feeding and suggest that the discriminating volatile organic compounds could be exploited in attractive toxic sugar- and odor- bait technologies control strategies.
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spelling pubmed-78953822021-03-01 Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya Hassaballa, Iman B. Sole, Catherine L. Cheseto, Xavier Torto, Baldwyn Tchouassi, David P. PLoS Negl Trop Dis Research Article The bioecology of phlebotomine sand flies is intimately linked to the utilization of environmental resources including plant feeding. However, plant feeding behavior of sand flies remains largely understudied for Afrotropical species. Here, using a combination of biochemical, molecular, and chemical approaches, we decipher specific plant-feeding associations in field-collected sand flies from a dry ecology endemic for leishmaniasis in Kenya. Cold-anthrone test indicative of recent plant feeding showed that fructose positivity rates were similar in both sand fly sexes and between those sampled indoors and outdoors. Analysis of derived sequences of the ribulose-1,5-bisphosphate carboxylase large subunit gene (rbcL) from fructose-positive specimens implicated mainly Acacia plants in the family Fabaceae (73%) as those readily foraged on by both sexes of Phlebotomus and Sergentomyia. Chemical analysis by high performance liquid chromatography detected fructose as the most common sugar in sand flies and leaves of selected plant species in the Fabaceae family. Analysis of similarities (ANOSIM) of the headspace volatile profiles of selected Fabaceae plants identified benzyl alcohol, (Z)-linalool oxide, (E)-β-ocimene, p-cymene, p-cresol, and m-cresol, as discriminating compounds between the plant volatiles. These results indicate selective sand fly plant feeding and suggest that the discriminating volatile organic compounds could be exploited in attractive toxic sugar- and odor- bait technologies control strategies. Public Library of Science 2021-02-08 /pmc/articles/PMC7895382/ /pubmed/33556068 http://dx.doi.org/10.1371/journal.pntd.0009041 Text en © 2021 Hassaballa et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hassaballa, Iman B.
Sole, Catherine L.
Cheseto, Xavier
Torto, Baldwyn
Tchouassi, David P.
Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya
title Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya
title_full Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya
title_fullStr Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya
title_full_unstemmed Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya
title_short Afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, Kenya
title_sort afrotropical sand fly-host plant relationships in a leishmaniasis endemic area, kenya
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895382/
https://www.ncbi.nlm.nih.gov/pubmed/33556068
http://dx.doi.org/10.1371/journal.pntd.0009041
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