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Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy
Drug-induced allergy (DIA), an unexpectedly triggered side effect of drugs used for therapeutic purposes, is a serious clinical issue that needs to be resolved because it interrupts the treatment of the primary disease. Since conventional allergy testing is insufficient to accurately predict the occ...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895410/ https://www.ncbi.nlm.nih.gov/pubmed/33606707 http://dx.doi.org/10.1371/journal.pone.0246125 |
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author | Shibaguchi, Hirotomo Yasutaka, Yuki Futagami, Koujiro |
author_facet | Shibaguchi, Hirotomo Yasutaka, Yuki Futagami, Koujiro |
author_sort | Shibaguchi, Hirotomo |
collection | PubMed |
description | Drug-induced allergy (DIA), an unexpectedly triggered side effect of drugs used for therapeutic purposes, is a serious clinical issue that needs to be resolved because it interrupts the treatment of the primary disease. Since conventional allergy testing is insufficient to accurately predict the occurrence of DIA or to determine the drugs causing it, the development of diagnostic and predictive tools for allergic reactions is important. We demonstrated a novel method, termed high-sensitive allergy test (HiSAT), for the rapid diagnosis of allergy (within 1 hr; with true-positive diagnosis rates of 89% and 9% for patients with and without allergy-like symptoms, respectively). HiSAT analyzes the cell kinetics as an index against chemotactic factors in a patient’s serum, as different from the diagnosis using conventional methods. Once allergy has occurred, HiSAT can be used to determine the causative medicine using culture supernatants incubated with the subject’s lymphocytes and the test allergen. This test is more efficient (60%) than the lymphocyte transformation test (20%). Furthermore, in HiSAT, cell mobility significantly increases in a dose-dependent manner against supernatant incubated with lymphocytes from a subject with pollinosis collected at a time when the subject is without allergic symptoms and the antigen. The result demonstraed that HiSAT might be a promising method to rapidly diagnose DIA or to determine with high accuracy the antigen causing allergy. |
format | Online Article Text |
id | pubmed-7895410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78954102021-03-01 Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy Shibaguchi, Hirotomo Yasutaka, Yuki Futagami, Koujiro PLoS One Research Article Drug-induced allergy (DIA), an unexpectedly triggered side effect of drugs used for therapeutic purposes, is a serious clinical issue that needs to be resolved because it interrupts the treatment of the primary disease. Since conventional allergy testing is insufficient to accurately predict the occurrence of DIA or to determine the drugs causing it, the development of diagnostic and predictive tools for allergic reactions is important. We demonstrated a novel method, termed high-sensitive allergy test (HiSAT), for the rapid diagnosis of allergy (within 1 hr; with true-positive diagnosis rates of 89% and 9% for patients with and without allergy-like symptoms, respectively). HiSAT analyzes the cell kinetics as an index against chemotactic factors in a patient’s serum, as different from the diagnosis using conventional methods. Once allergy has occurred, HiSAT can be used to determine the causative medicine using culture supernatants incubated with the subject’s lymphocytes and the test allergen. This test is more efficient (60%) than the lymphocyte transformation test (20%). Furthermore, in HiSAT, cell mobility significantly increases in a dose-dependent manner against supernatant incubated with lymphocytes from a subject with pollinosis collected at a time when the subject is without allergic symptoms and the antigen. The result demonstraed that HiSAT might be a promising method to rapidly diagnose DIA or to determine with high accuracy the antigen causing allergy. Public Library of Science 2021-02-19 /pmc/articles/PMC7895410/ /pubmed/33606707 http://dx.doi.org/10.1371/journal.pone.0246125 Text en © 2021 Shibaguchi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shibaguchi, Hirotomo Yasutaka, Yuki Futagami, Koujiro Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy |
title | Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy |
title_full | Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy |
title_fullStr | Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy |
title_full_unstemmed | Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy |
title_short | Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy |
title_sort | novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895410/ https://www.ncbi.nlm.nih.gov/pubmed/33606707 http://dx.doi.org/10.1371/journal.pone.0246125 |
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