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Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy

Drug-induced allergy (DIA), an unexpectedly triggered side effect of drugs used for therapeutic purposes, is a serious clinical issue that needs to be resolved because it interrupts the treatment of the primary disease. Since conventional allergy testing is insufficient to accurately predict the occ...

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Detalles Bibliográficos
Autores principales: Shibaguchi, Hirotomo, Yasutaka, Yuki, Futagami, Koujiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895410/
https://www.ncbi.nlm.nih.gov/pubmed/33606707
http://dx.doi.org/10.1371/journal.pone.0246125
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author Shibaguchi, Hirotomo
Yasutaka, Yuki
Futagami, Koujiro
author_facet Shibaguchi, Hirotomo
Yasutaka, Yuki
Futagami, Koujiro
author_sort Shibaguchi, Hirotomo
collection PubMed
description Drug-induced allergy (DIA), an unexpectedly triggered side effect of drugs used for therapeutic purposes, is a serious clinical issue that needs to be resolved because it interrupts the treatment of the primary disease. Since conventional allergy testing is insufficient to accurately predict the occurrence of DIA or to determine the drugs causing it, the development of diagnostic and predictive tools for allergic reactions is important. We demonstrated a novel method, termed high-sensitive allergy test (HiSAT), for the rapid diagnosis of allergy (within 1 hr; with true-positive diagnosis rates of 89% and 9% for patients with and without allergy-like symptoms, respectively). HiSAT analyzes the cell kinetics as an index against chemotactic factors in a patient’s serum, as different from the diagnosis using conventional methods. Once allergy has occurred, HiSAT can be used to determine the causative medicine using culture supernatants incubated with the subject’s lymphocytes and the test allergen. This test is more efficient (60%) than the lymphocyte transformation test (20%). Furthermore, in HiSAT, cell mobility significantly increases in a dose-dependent manner against supernatant incubated with lymphocytes from a subject with pollinosis collected at a time when the subject is without allergic symptoms and the antigen. The result demonstraed that HiSAT might be a promising method to rapidly diagnose DIA or to determine with high accuracy the antigen causing allergy.
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spelling pubmed-78954102021-03-01 Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy Shibaguchi, Hirotomo Yasutaka, Yuki Futagami, Koujiro PLoS One Research Article Drug-induced allergy (DIA), an unexpectedly triggered side effect of drugs used for therapeutic purposes, is a serious clinical issue that needs to be resolved because it interrupts the treatment of the primary disease. Since conventional allergy testing is insufficient to accurately predict the occurrence of DIA or to determine the drugs causing it, the development of diagnostic and predictive tools for allergic reactions is important. We demonstrated a novel method, termed high-sensitive allergy test (HiSAT), for the rapid diagnosis of allergy (within 1 hr; with true-positive diagnosis rates of 89% and 9% for patients with and without allergy-like symptoms, respectively). HiSAT analyzes the cell kinetics as an index against chemotactic factors in a patient’s serum, as different from the diagnosis using conventional methods. Once allergy has occurred, HiSAT can be used to determine the causative medicine using culture supernatants incubated with the subject’s lymphocytes and the test allergen. This test is more efficient (60%) than the lymphocyte transformation test (20%). Furthermore, in HiSAT, cell mobility significantly increases in a dose-dependent manner against supernatant incubated with lymphocytes from a subject with pollinosis collected at a time when the subject is without allergic symptoms and the antigen. The result demonstraed that HiSAT might be a promising method to rapidly diagnose DIA or to determine with high accuracy the antigen causing allergy. Public Library of Science 2021-02-19 /pmc/articles/PMC7895410/ /pubmed/33606707 http://dx.doi.org/10.1371/journal.pone.0246125 Text en © 2021 Shibaguchi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shibaguchi, Hirotomo
Yasutaka, Yuki
Futagami, Koujiro
Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy
title Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy
title_full Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy
title_fullStr Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy
title_full_unstemmed Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy
title_short Novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy
title_sort novel method to analyze cell kinetics for the rapid diagnosis and determination of the causative agent in allergy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895410/
https://www.ncbi.nlm.nih.gov/pubmed/33606707
http://dx.doi.org/10.1371/journal.pone.0246125
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