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Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury
Flavonoids have been reported to have therapeutic potential for spinal cord injury. Hawthorn leaves have abundant content and species of total flavonoids, and studies of the effects of the total flavonoids of hawthorn leaves on spinal cord injury have not been published in or outside China. Therefor...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896213/ https://www.ncbi.nlm.nih.gov/pubmed/32859797 http://dx.doi.org/10.4103/1673-5374.286975 |
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author | Zhang, Qiong Xiong, Yin Li, Bo Deng, Gui-Ying Fu, Wen-Wen Cao, Bai-Chuan Zong, Shao-Hui Zeng, Gao-Feng |
author_facet | Zhang, Qiong Xiong, Yin Li, Bo Deng, Gui-Ying Fu, Wen-Wen Cao, Bai-Chuan Zong, Shao-Hui Zeng, Gao-Feng |
author_sort | Zhang, Qiong |
collection | PubMed |
description | Flavonoids have been reported to have therapeutic potential for spinal cord injury. Hawthorn leaves have abundant content and species of total flavonoids, and studies of the effects of the total flavonoids of hawthorn leaves on spinal cord injury have not been published in or outside China. Therefore, Sprague-Dawley rats were used to establish a spinal cord injury model by Allen’s method. Rats were intraperitoneally injected with 0.2 mL of different concentrations of total flavonoids of hawthorn leaves (5, 10, and 20 mg/kg) after spinal cord injury. Injections were administered once every 6 hours, three times a day, for 14 days. After treatment with various concentrations of total flavonoids of hawthorn leaves, the Basso, Beattie, and Bresnahan scores and histological staining indicated decreases in the lesion cavity and number of apoptotic cells of the injured spinal cord tissue; the morphological arrangement of the myelin sheath and nerve cells tended to be regular; and the Nissl bodies in neurons increased. The Basso, Beattie, and Bresnahan scores of treated spinal cord injury rats were increased. Western blot assays showed that the expression levels of pro-apoptotic Bax and cleaved caspase-3 were decreased, but the expression level of the anti-apoptotic Bcl-2 protein was increased. The improvement of the above physiological indicators showed a dose-dependent relationship with the concentration of total flavonoids of hawthorn leaves. The above findings confirm that total flavonoids of hawthorn leaves can reduce apoptosis and exert neuroprotective effects to promote the recovery of the motor function of rats with spinal cord injury. This study was approved by the Ethics Committee of the Guangxi Medical University of China (approval No. 201810042) in October 2018. |
format | Online Article Text |
id | pubmed-7896213 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-78962132021-02-24 Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury Zhang, Qiong Xiong, Yin Li, Bo Deng, Gui-Ying Fu, Wen-Wen Cao, Bai-Chuan Zong, Shao-Hui Zeng, Gao-Feng Neural Regen Res Research Article Flavonoids have been reported to have therapeutic potential for spinal cord injury. Hawthorn leaves have abundant content and species of total flavonoids, and studies of the effects of the total flavonoids of hawthorn leaves on spinal cord injury have not been published in or outside China. Therefore, Sprague-Dawley rats were used to establish a spinal cord injury model by Allen’s method. Rats were intraperitoneally injected with 0.2 mL of different concentrations of total flavonoids of hawthorn leaves (5, 10, and 20 mg/kg) after spinal cord injury. Injections were administered once every 6 hours, three times a day, for 14 days. After treatment with various concentrations of total flavonoids of hawthorn leaves, the Basso, Beattie, and Bresnahan scores and histological staining indicated decreases in the lesion cavity and number of apoptotic cells of the injured spinal cord tissue; the morphological arrangement of the myelin sheath and nerve cells tended to be regular; and the Nissl bodies in neurons increased. The Basso, Beattie, and Bresnahan scores of treated spinal cord injury rats were increased. Western blot assays showed that the expression levels of pro-apoptotic Bax and cleaved caspase-3 were decreased, but the expression level of the anti-apoptotic Bcl-2 protein was increased. The improvement of the above physiological indicators showed a dose-dependent relationship with the concentration of total flavonoids of hawthorn leaves. The above findings confirm that total flavonoids of hawthorn leaves can reduce apoptosis and exert neuroprotective effects to promote the recovery of the motor function of rats with spinal cord injury. This study was approved by the Ethics Committee of the Guangxi Medical University of China (approval No. 201810042) in October 2018. Wolters Kluwer - Medknow 2020-08-24 /pmc/articles/PMC7896213/ /pubmed/32859797 http://dx.doi.org/10.4103/1673-5374.286975 Text en Copyright: © 2021 Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Zhang, Qiong Xiong, Yin Li, Bo Deng, Gui-Ying Fu, Wen-Wen Cao, Bai-Chuan Zong, Shao-Hui Zeng, Gao-Feng Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury |
title | Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury |
title_full | Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury |
title_fullStr | Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury |
title_full_unstemmed | Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury |
title_short | Total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury |
title_sort | total flavonoids of hawthorn leaves promote motor function recovery via inhibition of apoptosis after spinal cord injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896213/ https://www.ncbi.nlm.nih.gov/pubmed/32859797 http://dx.doi.org/10.4103/1673-5374.286975 |
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