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A novel stabilization mechanism for the type VI secretion system sheath
The type VI secretion system (T6SS) is a phage-derived contractile nanomachine primarily involved in interbacterial competition. Its pivotal component, TssA, is indispensable for the assembly of the T6SS sheath structure, the contraction of which propels a payload of effector proteins into neighbori...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896307/ https://www.ncbi.nlm.nih.gov/pubmed/33558227 http://dx.doi.org/10.1073/pnas.2008500118 |
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author | Bernal, Patricia Furniss, R. Christopher D. Fecht, Selina Leung, Rhoda C. Y. Spiga, Livia Mavridou, Despoina A. I. Filloux, Alain |
author_facet | Bernal, Patricia Furniss, R. Christopher D. Fecht, Selina Leung, Rhoda C. Y. Spiga, Livia Mavridou, Despoina A. I. Filloux, Alain |
author_sort | Bernal, Patricia |
collection | PubMed |
description | The type VI secretion system (T6SS) is a phage-derived contractile nanomachine primarily involved in interbacterial competition. Its pivotal component, TssA, is indispensable for the assembly of the T6SS sheath structure, the contraction of which propels a payload of effector proteins into neighboring cells. Despite their key function, TssA proteins exhibit unexpected diversity and exist in two major forms, a short form (TssA(S)) and a long form (TssA(L)). While TssA(L) proteins interact with a partner, called TagA, to anchor the distal end of the extended sheath, the mechanism for the stabilization of TssA(S)-containing T6SSs remains unknown. Here we discover a class of structural components that interact with short TssA proteins and contribute to T6SS assembly by stabilizing the polymerizing sheath from the baseplate. We demonstrate that the presence of these components is important for full sheath extension and optimal firing. Moreover, we show that the pairing of each form of TssA with a different class of sheath stabilization proteins results in T6SS apparatuses that either reside in the cell for some time or fire immediately after sheath extension. We propose that this diversity in firing dynamics could contribute to the specialization of the T6SS to suit bacterial lifestyles in diverse environmental niches. |
format | Online Article Text |
id | pubmed-7896307 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-78963072021-02-24 A novel stabilization mechanism for the type VI secretion system sheath Bernal, Patricia Furniss, R. Christopher D. Fecht, Selina Leung, Rhoda C. Y. Spiga, Livia Mavridou, Despoina A. I. Filloux, Alain Proc Natl Acad Sci U S A Biological Sciences The type VI secretion system (T6SS) is a phage-derived contractile nanomachine primarily involved in interbacterial competition. Its pivotal component, TssA, is indispensable for the assembly of the T6SS sheath structure, the contraction of which propels a payload of effector proteins into neighboring cells. Despite their key function, TssA proteins exhibit unexpected diversity and exist in two major forms, a short form (TssA(S)) and a long form (TssA(L)). While TssA(L) proteins interact with a partner, called TagA, to anchor the distal end of the extended sheath, the mechanism for the stabilization of TssA(S)-containing T6SSs remains unknown. Here we discover a class of structural components that interact with short TssA proteins and contribute to T6SS assembly by stabilizing the polymerizing sheath from the baseplate. We demonstrate that the presence of these components is important for full sheath extension and optimal firing. Moreover, we show that the pairing of each form of TssA with a different class of sheath stabilization proteins results in T6SS apparatuses that either reside in the cell for some time or fire immediately after sheath extension. We propose that this diversity in firing dynamics could contribute to the specialization of the T6SS to suit bacterial lifestyles in diverse environmental niches. National Academy of Sciences 2021-02-16 2021-02-08 /pmc/articles/PMC7896307/ /pubmed/33558227 http://dx.doi.org/10.1073/pnas.2008500118 Text en Copyright © 2021 the Author(s). Published by PNAS. http://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Biological Sciences Bernal, Patricia Furniss, R. Christopher D. Fecht, Selina Leung, Rhoda C. Y. Spiga, Livia Mavridou, Despoina A. I. Filloux, Alain A novel stabilization mechanism for the type VI secretion system sheath |
title | A novel stabilization mechanism for the type VI secretion system sheath |
title_full | A novel stabilization mechanism for the type VI secretion system sheath |
title_fullStr | A novel stabilization mechanism for the type VI secretion system sheath |
title_full_unstemmed | A novel stabilization mechanism for the type VI secretion system sheath |
title_short | A novel stabilization mechanism for the type VI secretion system sheath |
title_sort | novel stabilization mechanism for the type vi secretion system sheath |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896307/ https://www.ncbi.nlm.nih.gov/pubmed/33558227 http://dx.doi.org/10.1073/pnas.2008500118 |
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