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Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India

BACKGROUND AND AIM: Selection and dissemination of plasmid-encoded extended-spectrum β-lactamase (ESBL) among Enterobacteriaceae confers resistance to beta-lactam antibiotics. The purpose of this study was to determine the prevalence and molecular characteristics of ESBL-producing organisms isolated...

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Autores principales: Agrawal, Samiksha, Singh, Ajay Pratap, Singh, Rashmi, Saikia, Raktim, Choudhury, Soumen, Shukla, Amit, Prabhu, Shyama N., Agrawal, Jitendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896901/
https://www.ncbi.nlm.nih.gov/pubmed/33642805
http://dx.doi.org/10.14202/vetworld.2021.200-209
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author Agrawal, Samiksha
Singh, Ajay Pratap
Singh, Rashmi
Saikia, Raktim
Choudhury, Soumen
Shukla, Amit
Prabhu, Shyama N.
Agrawal, Jitendra
author_facet Agrawal, Samiksha
Singh, Ajay Pratap
Singh, Rashmi
Saikia, Raktim
Choudhury, Soumen
Shukla, Amit
Prabhu, Shyama N.
Agrawal, Jitendra
author_sort Agrawal, Samiksha
collection PubMed
description BACKGROUND AND AIM: Selection and dissemination of plasmid-encoded extended-spectrum β-lactamase (ESBL) among Enterobacteriaceae confers resistance to beta-lactam antibiotics. The purpose of this study was to determine the prevalence and molecular characteristics of ESBL-producing organisms isolated from dairy cattle with a uterine infection. MATERIALS AND METHODS: Bacterial isolates (n=62) were characterized by biochemical test for genus and species determination. Antimicrobial susceptibility tests were performed by Kirby–Bauer disk diffusion method using panel of antibiotics for initial screening of ESBL organism. Phenotypic confirmation of ESBL-suspected strains was done by combination disk method and double-disk method. Multiplex polymerase chain reaction (PCR) was carried out for phylogrouping of Escherichia coli isolates as well as for genotyping ESBL genes. Enterobacterial repetitive intergenic consensus-PCR method was used for genotypic characterization of isolates. RESULTS: Antibiotic susceptibility profile of E. coli (n=40) isolates showed high rates of resistance for ampicillin (95.0%), cefpodoxime (97.5%), cefotaxime (87.5%), and ceftriaxone (70%). However, low rates of resistance were observed for cefoxitin (25%), amoxicillin/clavulanic acid (20%), ceftazidime (17.5%), gentamicin (10%), and ertapenem (7.5%). A total of 39/40 E. coli isolates were confirmed as ESBL with Epsilometer test as well as the genotypic method and 28 (70%) of them were multidrug-resistant. Genotype bla(CTX-M) was observed as a predominant beta-lactamase type with the preponderance of CTX-M Group 1. The following combinations were observed: bla(TEM)+ bla(CTX-M) in 15 (36.2%) isolates, bla(TEM)/bla(SHV) in 8 (5.2%) isolates, and bla(CTX-M)/bla(SHV) in 6 (5.2%) isolates. The phylogenetic grouping of E. coli strains revealed the highest prevalence for B1 (22.0%) followed by A (20%). CONCLUSION: This report shows a high frequency of ESBL E. coli from cattle with postpartum uterine infections. These isolates showed reduced susceptibility to common antibiotics used for the treatment of uterine infections greater affecting the therapeutic outcome.
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spelling pubmed-78969012021-02-26 Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India Agrawal, Samiksha Singh, Ajay Pratap Singh, Rashmi Saikia, Raktim Choudhury, Soumen Shukla, Amit Prabhu, Shyama N. Agrawal, Jitendra Vet World Research Article BACKGROUND AND AIM: Selection and dissemination of plasmid-encoded extended-spectrum β-lactamase (ESBL) among Enterobacteriaceae confers resistance to beta-lactam antibiotics. The purpose of this study was to determine the prevalence and molecular characteristics of ESBL-producing organisms isolated from dairy cattle with a uterine infection. MATERIALS AND METHODS: Bacterial isolates (n=62) were characterized by biochemical test for genus and species determination. Antimicrobial susceptibility tests were performed by Kirby–Bauer disk diffusion method using panel of antibiotics for initial screening of ESBL organism. Phenotypic confirmation of ESBL-suspected strains was done by combination disk method and double-disk method. Multiplex polymerase chain reaction (PCR) was carried out for phylogrouping of Escherichia coli isolates as well as for genotyping ESBL genes. Enterobacterial repetitive intergenic consensus-PCR method was used for genotypic characterization of isolates. RESULTS: Antibiotic susceptibility profile of E. coli (n=40) isolates showed high rates of resistance for ampicillin (95.0%), cefpodoxime (97.5%), cefotaxime (87.5%), and ceftriaxone (70%). However, low rates of resistance were observed for cefoxitin (25%), amoxicillin/clavulanic acid (20%), ceftazidime (17.5%), gentamicin (10%), and ertapenem (7.5%). A total of 39/40 E. coli isolates were confirmed as ESBL with Epsilometer test as well as the genotypic method and 28 (70%) of them were multidrug-resistant. Genotype bla(CTX-M) was observed as a predominant beta-lactamase type with the preponderance of CTX-M Group 1. The following combinations were observed: bla(TEM)+ bla(CTX-M) in 15 (36.2%) isolates, bla(TEM)/bla(SHV) in 8 (5.2%) isolates, and bla(CTX-M)/bla(SHV) in 6 (5.2%) isolates. The phylogenetic grouping of E. coli strains revealed the highest prevalence for B1 (22.0%) followed by A (20%). CONCLUSION: This report shows a high frequency of ESBL E. coli from cattle with postpartum uterine infections. These isolates showed reduced susceptibility to common antibiotics used for the treatment of uterine infections greater affecting the therapeutic outcome. Veterinary World 2021-01 2021-01-23 /pmc/articles/PMC7896901/ /pubmed/33642805 http://dx.doi.org/10.14202/vetworld.2021.200-209 Text en Copyright: © Agrawal, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Agrawal, Samiksha
Singh, Ajay Pratap
Singh, Rashmi
Saikia, Raktim
Choudhury, Soumen
Shukla, Amit
Prabhu, Shyama N.
Agrawal, Jitendra
Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India
title Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India
title_full Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India
title_fullStr Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India
title_full_unstemmed Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India
title_short Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from postpartum uterine infection in dairy cattle in India
title_sort molecular characterization of extended-spectrum β-lactamase-producing escherichia coli isolated from postpartum uterine infection in dairy cattle in india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896901/
https://www.ncbi.nlm.nih.gov/pubmed/33642805
http://dx.doi.org/10.14202/vetworld.2021.200-209
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