Detecting aspartate isomerization and backbone cleavage after aspartate in intact proteins by NMR spectroscopy

The monitoring of non-enzymatic post-translational modifications (PTMs) in therapeutic proteins is important to ensure drug safety and efficacy. Together with methionine and asparagine, aspartic acid (Asp) is very sensitive to spontaneous alterations. In particular, Asp residues can undergo isomeriz...

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Detalles Bibliográficos
Autores principales: Hinterholzer, Arthur, Stanojlovic, Vesna, Regl, Christof, Huber, Christian G., Cabrele, Chiara, Schubert, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897204/
https://www.ncbi.nlm.nih.gov/pubmed/33475951
http://dx.doi.org/10.1007/s10858-020-00356-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897204/
https://www.ncbi.nlm.nih.gov/pubmed/33475951
http://dx.doi.org/10.1007/s10858-020-00356-4

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