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Bulges in left-handed G-quadruplexes
G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897477/ https://www.ncbi.nlm.nih.gov/pubmed/33503265 http://dx.doi.org/10.1093/nar/gkaa1259 |
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author | Das, Poulomi Ngo, Khac Huy Winnerdy, Fernaldo Richtia Maity, Arijit Bakalar, Blaž Mechulam, Yves Schmitt, Emmanuelle Phan, Anh Tuân |
author_facet | Das, Poulomi Ngo, Khac Huy Winnerdy, Fernaldo Richtia Maity, Arijit Bakalar, Blaž Mechulam, Yves Schmitt, Emmanuelle Phan, Anh Tuân |
author_sort | Das, Poulomi |
collection | PubMed |
description | G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures. |
format | Online Article Text |
id | pubmed-7897477 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-78974772021-02-25 Bulges in left-handed G-quadruplexes Das, Poulomi Ngo, Khac Huy Winnerdy, Fernaldo Richtia Maity, Arijit Bakalar, Blaž Mechulam, Yves Schmitt, Emmanuelle Phan, Anh Tuân Nucleic Acids Res Structural Biology G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures. Oxford University Press 2021-01-27 /pmc/articles/PMC7897477/ /pubmed/33503265 http://dx.doi.org/10.1093/nar/gkaa1259 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Structural Biology Das, Poulomi Ngo, Khac Huy Winnerdy, Fernaldo Richtia Maity, Arijit Bakalar, Blaž Mechulam, Yves Schmitt, Emmanuelle Phan, Anh Tuân Bulges in left-handed G-quadruplexes |
title | Bulges in left-handed G-quadruplexes |
title_full | Bulges in left-handed G-quadruplexes |
title_fullStr | Bulges in left-handed G-quadruplexes |
title_full_unstemmed | Bulges in left-handed G-quadruplexes |
title_short | Bulges in left-handed G-quadruplexes |
title_sort | bulges in left-handed g-quadruplexes |
topic | Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897477/ https://www.ncbi.nlm.nih.gov/pubmed/33503265 http://dx.doi.org/10.1093/nar/gkaa1259 |
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