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Bulges in left-handed G-quadruplexes

G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the...

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Autores principales: Das, Poulomi, Ngo, Khac Huy, Winnerdy, Fernaldo Richtia, Maity, Arijit, Bakalar, Blaž, Mechulam, Yves, Schmitt, Emmanuelle, Phan, Anh Tuân
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897477/
https://www.ncbi.nlm.nih.gov/pubmed/33503265
http://dx.doi.org/10.1093/nar/gkaa1259
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author Das, Poulomi
Ngo, Khac Huy
Winnerdy, Fernaldo Richtia
Maity, Arijit
Bakalar, Blaž
Mechulam, Yves
Schmitt, Emmanuelle
Phan, Anh Tuân
author_facet Das, Poulomi
Ngo, Khac Huy
Winnerdy, Fernaldo Richtia
Maity, Arijit
Bakalar, Blaž
Mechulam, Yves
Schmitt, Emmanuelle
Phan, Anh Tuân
author_sort Das, Poulomi
collection PubMed
description G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.
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spelling pubmed-78974772021-02-25 Bulges in left-handed G-quadruplexes Das, Poulomi Ngo, Khac Huy Winnerdy, Fernaldo Richtia Maity, Arijit Bakalar, Blaž Mechulam, Yves Schmitt, Emmanuelle Phan, Anh Tuân Nucleic Acids Res Structural Biology G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures. Oxford University Press 2021-01-27 /pmc/articles/PMC7897477/ /pubmed/33503265 http://dx.doi.org/10.1093/nar/gkaa1259 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Structural Biology
Das, Poulomi
Ngo, Khac Huy
Winnerdy, Fernaldo Richtia
Maity, Arijit
Bakalar, Blaž
Mechulam, Yves
Schmitt, Emmanuelle
Phan, Anh Tuân
Bulges in left-handed G-quadruplexes
title Bulges in left-handed G-quadruplexes
title_full Bulges in left-handed G-quadruplexes
title_fullStr Bulges in left-handed G-quadruplexes
title_full_unstemmed Bulges in left-handed G-quadruplexes
title_short Bulges in left-handed G-quadruplexes
title_sort bulges in left-handed g-quadruplexes
topic Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897477/
https://www.ncbi.nlm.nih.gov/pubmed/33503265
http://dx.doi.org/10.1093/nar/gkaa1259
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