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Enhancement of morphine-induced antinociception after electroconvulsive shock in mice

Electroconvulsive therapy (ECT) has been applied for chronic pain for decades. The amounts of opioids to treat pain are sometimes reduced after a series of ECT. The effect of ECT on morphine-induced analgesia and its mechanism underlying the reduction of morphine requirement has yet to be clarified....

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Autores principales: Iwata, Ken, Takamatsu, Yukio, Doi, Nagafumi, Ikeda, Kazutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897827/
https://www.ncbi.nlm.nih.gov/pubmed/33599155
http://dx.doi.org/10.1177/1744806921992628
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author Iwata, Ken
Takamatsu, Yukio
Doi, Nagafumi
Ikeda, Kazutaka
author_facet Iwata, Ken
Takamatsu, Yukio
Doi, Nagafumi
Ikeda, Kazutaka
author_sort Iwata, Ken
collection PubMed
description Electroconvulsive therapy (ECT) has been applied for chronic pain for decades. The amounts of opioids to treat pain are sometimes reduced after a series of ECT. The effect of ECT on morphine-induced analgesia and its mechanism underlying the reduction of morphine requirement has yet to be clarified. Therefore, we administered electroconvulsive shocks (ECS) to mice and investigated the antinociceptive effect of morphine in a hot plate test. We examined the expression level of µ-opioid receptor in the thalami of mice 25 h after administration of ECS compared to the thalami of mice without ECS administration using western blotting. ECS disturbed the development of a decrease in the percentage of maximal possible effect (%MPE), which was observed 24 h after a morphine injection, when ECS was applied 25, 23, 21, and 12 h before the second administration of morphine. We also examined the effect of ECS on the dose-response curve of %MPE to morphine-antinociception. Twenty-five hours after ECS, the dose-response curve was shifted to the left, and the EC(50) of morphine given to ECS-pretreated mice decreased by 30.1% compared to the mice that were not pretreated with ECS. We also found that the expression level of µ-opioid receptors was significantly increased after ECS administration. These results confirm previous clinical reports showing that ECT decreased the required dose of opioids in neuropathic pain patients and suggest the hypothesis that this effect of ECT works through the thalamus.
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spelling pubmed-78978272021-03-04 Enhancement of morphine-induced antinociception after electroconvulsive shock in mice Iwata, Ken Takamatsu, Yukio Doi, Nagafumi Ikeda, Kazutaka Mol Pain Research Article Electroconvulsive therapy (ECT) has been applied for chronic pain for decades. The amounts of opioids to treat pain are sometimes reduced after a series of ECT. The effect of ECT on morphine-induced analgesia and its mechanism underlying the reduction of morphine requirement has yet to be clarified. Therefore, we administered electroconvulsive shocks (ECS) to mice and investigated the antinociceptive effect of morphine in a hot plate test. We examined the expression level of µ-opioid receptor in the thalami of mice 25 h after administration of ECS compared to the thalami of mice without ECS administration using western blotting. ECS disturbed the development of a decrease in the percentage of maximal possible effect (%MPE), which was observed 24 h after a morphine injection, when ECS was applied 25, 23, 21, and 12 h before the second administration of morphine. We also examined the effect of ECS on the dose-response curve of %MPE to morphine-antinociception. Twenty-five hours after ECS, the dose-response curve was shifted to the left, and the EC(50) of morphine given to ECS-pretreated mice decreased by 30.1% compared to the mice that were not pretreated with ECS. We also found that the expression level of µ-opioid receptors was significantly increased after ECS administration. These results confirm previous clinical reports showing that ECT decreased the required dose of opioids in neuropathic pain patients and suggest the hypothesis that this effect of ECT works through the thalamus. SAGE Publications 2021-02-18 /pmc/articles/PMC7897827/ /pubmed/33599155 http://dx.doi.org/10.1177/1744806921992628 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Article
Iwata, Ken
Takamatsu, Yukio
Doi, Nagafumi
Ikeda, Kazutaka
Enhancement of morphine-induced antinociception after electroconvulsive shock in mice
title Enhancement of morphine-induced antinociception after electroconvulsive shock in mice
title_full Enhancement of morphine-induced antinociception after electroconvulsive shock in mice
title_fullStr Enhancement of morphine-induced antinociception after electroconvulsive shock in mice
title_full_unstemmed Enhancement of morphine-induced antinociception after electroconvulsive shock in mice
title_short Enhancement of morphine-induced antinociception after electroconvulsive shock in mice
title_sort enhancement of morphine-induced antinociception after electroconvulsive shock in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7897827/
https://www.ncbi.nlm.nih.gov/pubmed/33599155
http://dx.doi.org/10.1177/1744806921992628
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