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Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro
Oxidative stress which can be induced by cigarette smoke (CS) is associated with an altered osteoblast differentiation, and an inhibition of the mineralization process. Therefore, treatments focusing on reducing oxidative stress in osteoblasts could be a potential therapy supporting bone formation....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Leibniz Research Centre for Working Environment and Human Factors
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898044/ https://www.ncbi.nlm.nih.gov/pubmed/33628164 http://dx.doi.org/10.17179/excli2020-3244 |
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author | Zhu, Sheng Aspera-Werz, Romina H. Chen, Tao Weng, Weidong Braun, Bianca Histing, Tina Nüssler, Andreas K. |
author_facet | Zhu, Sheng Aspera-Werz, Romina H. Chen, Tao Weng, Weidong Braun, Bianca Histing, Tina Nüssler, Andreas K. |
author_sort | Zhu, Sheng |
collection | PubMed |
description | Oxidative stress which can be induced by cigarette smoke (CS) is associated with an altered osteoblast differentiation, and an inhibition of the mineralization process. Therefore, treatments focusing on reducing oxidative stress in osteoblasts could be a potential therapy supporting bone formation. Maqui berry extract (MBE) is the richest natural source of delphinidins with high antioxidant activity. In the present study, we pre-/ co-/ post-incubated MBE in cigarette smoke extract (CSE)-affected human osteoblasts (hOBs), to investigate the effects of MBE as an antioxidant on hOBs. Our results clearly showed that high concentrations of MBE are toxic for hOBs, while physiological concentrations of MBE have no negative effects in vitro. Physiological concentrations of MBE can reduce oxidative stress caused by CSE in hOBs by activating the antioxidative regulator Nrf2 and its regulated antioxidative enzymes. Moreover, the physiological concentration of MBE prevents the detrimental effects of CSE-induced oxidative damage on hOBs by increasing cell viability, differentiation capability and matrix mineralization. Pre-incubation with MBE showed a positive effect on the activation of the cellular antioxidant system in hOBs. Thus, we conclude that MBE at physiological concentrations can effectively protect osteoblasts from oxidative stress-induced damage by activating the cells' antioxidative defense system. |
format | Online Article Text |
id | pubmed-7898044 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Leibniz Research Centre for Working Environment and Human Factors |
record_format | MEDLINE/PubMed |
spelling | pubmed-78980442021-02-23 Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro Zhu, Sheng Aspera-Werz, Romina H. Chen, Tao Weng, Weidong Braun, Bianca Histing, Tina Nüssler, Andreas K. EXCLI J Original Article Oxidative stress which can be induced by cigarette smoke (CS) is associated with an altered osteoblast differentiation, and an inhibition of the mineralization process. Therefore, treatments focusing on reducing oxidative stress in osteoblasts could be a potential therapy supporting bone formation. Maqui berry extract (MBE) is the richest natural source of delphinidins with high antioxidant activity. In the present study, we pre-/ co-/ post-incubated MBE in cigarette smoke extract (CSE)-affected human osteoblasts (hOBs), to investigate the effects of MBE as an antioxidant on hOBs. Our results clearly showed that high concentrations of MBE are toxic for hOBs, while physiological concentrations of MBE have no negative effects in vitro. Physiological concentrations of MBE can reduce oxidative stress caused by CSE in hOBs by activating the antioxidative regulator Nrf2 and its regulated antioxidative enzymes. Moreover, the physiological concentration of MBE prevents the detrimental effects of CSE-induced oxidative damage on hOBs by increasing cell viability, differentiation capability and matrix mineralization. Pre-incubation with MBE showed a positive effect on the activation of the cellular antioxidant system in hOBs. Thus, we conclude that MBE at physiological concentrations can effectively protect osteoblasts from oxidative stress-induced damage by activating the cells' antioxidative defense system. Leibniz Research Centre for Working Environment and Human Factors 2021-02-09 /pmc/articles/PMC7898044/ /pubmed/33628164 http://dx.doi.org/10.17179/excli2020-3244 Text en Copyright © 2021 Zhu et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited. |
spellingShingle | Original Article Zhu, Sheng Aspera-Werz, Romina H. Chen, Tao Weng, Weidong Braun, Bianca Histing, Tina Nüssler, Andreas K. Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro |
title | Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro |
title_full | Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro |
title_fullStr | Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro |
title_full_unstemmed | Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro |
title_short | Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro |
title_sort | maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898044/ https://www.ncbi.nlm.nih.gov/pubmed/33628164 http://dx.doi.org/10.17179/excli2020-3244 |
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