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Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro

Oxidative stress which can be induced by cigarette smoke (CS) is associated with an altered osteoblast differentiation, and an inhibition of the mineralization process. Therefore, treatments focusing on reducing oxidative stress in osteoblasts could be a potential therapy supporting bone formation....

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Autores principales: Zhu, Sheng, Aspera-Werz, Romina H., Chen, Tao, Weng, Weidong, Braun, Bianca, Histing, Tina, Nüssler, Andreas K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Leibniz Research Centre for Working Environment and Human Factors 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898044/
https://www.ncbi.nlm.nih.gov/pubmed/33628164
http://dx.doi.org/10.17179/excli2020-3244
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author Zhu, Sheng
Aspera-Werz, Romina H.
Chen, Tao
Weng, Weidong
Braun, Bianca
Histing, Tina
Nüssler, Andreas K.
author_facet Zhu, Sheng
Aspera-Werz, Romina H.
Chen, Tao
Weng, Weidong
Braun, Bianca
Histing, Tina
Nüssler, Andreas K.
author_sort Zhu, Sheng
collection PubMed
description Oxidative stress which can be induced by cigarette smoke (CS) is associated with an altered osteoblast differentiation, and an inhibition of the mineralization process. Therefore, treatments focusing on reducing oxidative stress in osteoblasts could be a potential therapy supporting bone formation. Maqui berry extract (MBE) is the richest natural source of delphinidins with high antioxidant activity. In the present study, we pre-/ co-/ post-incubated MBE in cigarette smoke extract (CSE)-affected human osteoblasts (hOBs), to investigate the effects of MBE as an antioxidant on hOBs. Our results clearly showed that high concentrations of MBE are toxic for hOBs, while physiological concentrations of MBE have no negative effects in vitro. Physiological concentrations of MBE can reduce oxidative stress caused by CSE in hOBs by activating the antioxidative regulator Nrf2 and its regulated antioxidative enzymes. Moreover, the physiological concentration of MBE prevents the detrimental effects of CSE-induced oxidative damage on hOBs by increasing cell viability, differentiation capability and matrix mineralization. Pre-incubation with MBE showed a positive effect on the activation of the cellular antioxidant system in hOBs. Thus, we conclude that MBE at physiological concentrations can effectively protect osteoblasts from oxidative stress-induced damage by activating the cells' antioxidative defense system.
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spelling pubmed-78980442021-02-23 Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro Zhu, Sheng Aspera-Werz, Romina H. Chen, Tao Weng, Weidong Braun, Bianca Histing, Tina Nüssler, Andreas K. EXCLI J Original Article Oxidative stress which can be induced by cigarette smoke (CS) is associated with an altered osteoblast differentiation, and an inhibition of the mineralization process. Therefore, treatments focusing on reducing oxidative stress in osteoblasts could be a potential therapy supporting bone formation. Maqui berry extract (MBE) is the richest natural source of delphinidins with high antioxidant activity. In the present study, we pre-/ co-/ post-incubated MBE in cigarette smoke extract (CSE)-affected human osteoblasts (hOBs), to investigate the effects of MBE as an antioxidant on hOBs. Our results clearly showed that high concentrations of MBE are toxic for hOBs, while physiological concentrations of MBE have no negative effects in vitro. Physiological concentrations of MBE can reduce oxidative stress caused by CSE in hOBs by activating the antioxidative regulator Nrf2 and its regulated antioxidative enzymes. Moreover, the physiological concentration of MBE prevents the detrimental effects of CSE-induced oxidative damage on hOBs by increasing cell viability, differentiation capability and matrix mineralization. Pre-incubation with MBE showed a positive effect on the activation of the cellular antioxidant system in hOBs. Thus, we conclude that MBE at physiological concentrations can effectively protect osteoblasts from oxidative stress-induced damage by activating the cells' antioxidative defense system. Leibniz Research Centre for Working Environment and Human Factors 2021-02-09 /pmc/articles/PMC7898044/ /pubmed/33628164 http://dx.doi.org/10.17179/excli2020-3244 Text en Copyright © 2021 Zhu et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited.
spellingShingle Original Article
Zhu, Sheng
Aspera-Werz, Romina H.
Chen, Tao
Weng, Weidong
Braun, Bianca
Histing, Tina
Nüssler, Andreas K.
Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro
title Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro
title_full Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro
title_fullStr Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro
title_full_unstemmed Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro
title_short Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro
title_sort maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblasts in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898044/
https://www.ncbi.nlm.nih.gov/pubmed/33628164
http://dx.doi.org/10.17179/excli2020-3244
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