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Detection and disinfection of COVID-19 virus in wastewater
The coronavirus disease 2019, COVID-19, caused by the severe acute respiratory syndrome coronavirus 2, SARS-CoV-2, appears as a major pandemic having adverse impact on public health and economic activities. Since viral replication in human enterocytes results in its faecal shedding, wastewater surve...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898499/ https://www.ncbi.nlm.nih.gov/pubmed/33642964 http://dx.doi.org/10.1007/s10311-021-01202-1 |
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author | Singh, Simranjeet Kumar, Vijay Kapoor, Dhriti Dhanjal, Daljeet Singh Bhatia, Deepika Jan, Sadaf Singh, Nasib Romero, Romina Ramamurthy, Praveen C. Singh, Joginder |
author_facet | Singh, Simranjeet Kumar, Vijay Kapoor, Dhriti Dhanjal, Daljeet Singh Bhatia, Deepika Jan, Sadaf Singh, Nasib Romero, Romina Ramamurthy, Praveen C. Singh, Joginder |
author_sort | Singh, Simranjeet |
collection | PubMed |
description | The coronavirus disease 2019, COVID-19, caused by the severe acute respiratory syndrome coronavirus 2, SARS-CoV-2, appears as a major pandemic having adverse impact on public health and economic activities. Since viral replication in human enterocytes results in its faecal shedding, wastewater surveillance is an ideal, non-invasive, cost-effective and an early warning epidemiological approach to detect the genetic material of SARS-CoV-2. Here, we review techniques for the detection of SARS-CoV-2 in municipal wastewater, and disinfectants used to control viral spread. For detection, concentration of ribonucleic acid involves ultrafiltration, ultracentrifugation and polyethylene glycol precipitation. Identification is done by reverse transcriptase amplification, nucleic acid sequence-based amplification, helicase dependent amplification, loop-mediated isothermal amplification, recombinase polymerase amplification, high throughput screening and biosensor assays. Disinfectants include ultraviolet radiations, ozone, chlorine dioxide, hypochlorites and hydrogen peroxide. Wastewater surveillance data indicates viral presence within longer detection window, and provides transmission dynamics earlier than classical methods. This is particularly relevant for pre-symptomatic and asymptomatic COVID-19 cases. |
format | Online Article Text |
id | pubmed-7898499 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-78984992021-02-23 Detection and disinfection of COVID-19 virus in wastewater Singh, Simranjeet Kumar, Vijay Kapoor, Dhriti Dhanjal, Daljeet Singh Bhatia, Deepika Jan, Sadaf Singh, Nasib Romero, Romina Ramamurthy, Praveen C. Singh, Joginder Environ Chem Lett Review The coronavirus disease 2019, COVID-19, caused by the severe acute respiratory syndrome coronavirus 2, SARS-CoV-2, appears as a major pandemic having adverse impact on public health and economic activities. Since viral replication in human enterocytes results in its faecal shedding, wastewater surveillance is an ideal, non-invasive, cost-effective and an early warning epidemiological approach to detect the genetic material of SARS-CoV-2. Here, we review techniques for the detection of SARS-CoV-2 in municipal wastewater, and disinfectants used to control viral spread. For detection, concentration of ribonucleic acid involves ultrafiltration, ultracentrifugation and polyethylene glycol precipitation. Identification is done by reverse transcriptase amplification, nucleic acid sequence-based amplification, helicase dependent amplification, loop-mediated isothermal amplification, recombinase polymerase amplification, high throughput screening and biosensor assays. Disinfectants include ultraviolet radiations, ozone, chlorine dioxide, hypochlorites and hydrogen peroxide. Wastewater surveillance data indicates viral presence within longer detection window, and provides transmission dynamics earlier than classical methods. This is particularly relevant for pre-symptomatic and asymptomatic COVID-19 cases. Springer International Publishing 2021-02-22 2021 /pmc/articles/PMC7898499/ /pubmed/33642964 http://dx.doi.org/10.1007/s10311-021-01202-1 Text en © The Author(s), under exclusive licence to Springer Nature Switzerland AG part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Review Singh, Simranjeet Kumar, Vijay Kapoor, Dhriti Dhanjal, Daljeet Singh Bhatia, Deepika Jan, Sadaf Singh, Nasib Romero, Romina Ramamurthy, Praveen C. Singh, Joginder Detection and disinfection of COVID-19 virus in wastewater |
title | Detection and disinfection of COVID-19 virus in wastewater |
title_full | Detection and disinfection of COVID-19 virus in wastewater |
title_fullStr | Detection and disinfection of COVID-19 virus in wastewater |
title_full_unstemmed | Detection and disinfection of COVID-19 virus in wastewater |
title_short | Detection and disinfection of COVID-19 virus in wastewater |
title_sort | detection and disinfection of covid-19 virus in wastewater |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898499/ https://www.ncbi.nlm.nih.gov/pubmed/33642964 http://dx.doi.org/10.1007/s10311-021-01202-1 |
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