Cargando…

Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19

OBJECTIVES: This study compares the infectivity of SARS-CoV-2 in respiratory samples from patients with mild COVID-19 with those from hospitalized patients with severe bilateral pneumonia. In severe COVID-19, we also analysed the presence of neutralizing activity in paired sera. METHODS: We performe...

Descripción completa

Detalles Bibliográficos
Autores principales: Folgueira, Maria Dolores, Luczkowiak, Joanna, Lasala, Fátima, Pérez-Rivilla, Alfredo, Delgado, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898982/
https://www.ncbi.nlm.nih.gov/pubmed/33631334
http://dx.doi.org/10.1016/j.cmi.2021.02.014
_version_ 1783653976374247424
author Folgueira, Maria Dolores
Luczkowiak, Joanna
Lasala, Fátima
Pérez-Rivilla, Alfredo
Delgado, Rafael
author_facet Folgueira, Maria Dolores
Luczkowiak, Joanna
Lasala, Fátima
Pérez-Rivilla, Alfredo
Delgado, Rafael
author_sort Folgueira, Maria Dolores
collection PubMed
description OBJECTIVES: This study compares the infectivity of SARS-CoV-2 in respiratory samples from patients with mild COVID-19 with those from hospitalized patients with severe bilateral pneumonia. In severe COVID-19, we also analysed the presence of neutralizing activity in paired sera. METHODS: We performed cell cultures on 193 real-time reverse transcription polymerase chain reaction respiratory samples, positive for SARS-CoV-2, obtained from 189 patients at various times, from clinical diagnosis to follow-up. Eleven samples were obtained from asymptomatic individuals, 91 samples from 91 outpatients with mild forms of COVID-19 and 91 samples from 87 inpatients with severe pneumonia. In these patients, neutralizing activity was analysed in 30 paired sera collected after symptom onset >10 days. RESULTS: We detected a cytopathic effect (CPE) in 91/193 (47%) samples. Viral viability was maintained for up to 10 days in patients with mild COVID-19. In patients with severe COVID-19, the virus remained viable for up to 32 days after the onset of symptoms. Patients with severe COVID-19 presented infectious virus at a significantly higher rate in the samples with moderate to low viral load (cycle threshold value ≥ 26): 32/75 (43%) versus 14/63 (22%) for mild cases (p < 0.01). We observed a positive CPE despite the presence of clear neutralizing activity (NT50 > 1:1024 in 10% (3/30) of samples. DISCUSSION: Patients with severe COVID-19 might shed viable virus during prolonged periods of up to 4 weeks after symptom onset, even when presenting high cycle threshold values in their respiratory samples and despite having developed high neutralizing antibody titres.
format Online
Article
Text
id pubmed-7898982
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd.
record_format MEDLINE/PubMed
spelling pubmed-78989822021-02-23 Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19 Folgueira, Maria Dolores Luczkowiak, Joanna Lasala, Fátima Pérez-Rivilla, Alfredo Delgado, Rafael Clin Microbiol Infect Original Article OBJECTIVES: This study compares the infectivity of SARS-CoV-2 in respiratory samples from patients with mild COVID-19 with those from hospitalized patients with severe bilateral pneumonia. In severe COVID-19, we also analysed the presence of neutralizing activity in paired sera. METHODS: We performed cell cultures on 193 real-time reverse transcription polymerase chain reaction respiratory samples, positive for SARS-CoV-2, obtained from 189 patients at various times, from clinical diagnosis to follow-up. Eleven samples were obtained from asymptomatic individuals, 91 samples from 91 outpatients with mild forms of COVID-19 and 91 samples from 87 inpatients with severe pneumonia. In these patients, neutralizing activity was analysed in 30 paired sera collected after symptom onset >10 days. RESULTS: We detected a cytopathic effect (CPE) in 91/193 (47%) samples. Viral viability was maintained for up to 10 days in patients with mild COVID-19. In patients with severe COVID-19, the virus remained viable for up to 32 days after the onset of symptoms. Patients with severe COVID-19 presented infectious virus at a significantly higher rate in the samples with moderate to low viral load (cycle threshold value ≥ 26): 32/75 (43%) versus 14/63 (22%) for mild cases (p < 0.01). We observed a positive CPE despite the presence of clear neutralizing activity (NT50 > 1:1024 in 10% (3/30) of samples. DISCUSSION: Patients with severe COVID-19 might shed viable virus during prolonged periods of up to 4 weeks after symptom onset, even when presenting high cycle threshold values in their respiratory samples and despite having developed high neutralizing antibody titres. European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. 2021-06 2021-02-22 /pmc/articles/PMC7898982/ /pubmed/33631334 http://dx.doi.org/10.1016/j.cmi.2021.02.014 Text en © 2021 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Original Article
Folgueira, Maria Dolores
Luczkowiak, Joanna
Lasala, Fátima
Pérez-Rivilla, Alfredo
Delgado, Rafael
Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19
title Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19
title_full Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19
title_fullStr Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19
title_full_unstemmed Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19
title_short Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19
title_sort prolonged sars-cov-2 cell culture replication in respiratory samples from patients with severe covid-19
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898982/
https://www.ncbi.nlm.nih.gov/pubmed/33631334
http://dx.doi.org/10.1016/j.cmi.2021.02.014
work_keys_str_mv AT folgueiramariadolores prolongedsarscov2cellculturereplicationinrespiratorysamplesfrompatientswithseverecovid19
AT luczkowiakjoanna prolongedsarscov2cellculturereplicationinrespiratorysamplesfrompatientswithseverecovid19
AT lasalafatima prolongedsarscov2cellculturereplicationinrespiratorysamplesfrompatientswithseverecovid19
AT perezrivillaalfredo prolongedsarscov2cellculturereplicationinrespiratorysamplesfrompatientswithseverecovid19
AT delgadorafael prolongedsarscov2cellculturereplicationinrespiratorysamplesfrompatientswithseverecovid19