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Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers

Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). The test has two steps: 1) heat saliva with a stabilization solution, and 2) detect virus by incubating with a primer/enzyme mix. After incubation, saliv...

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Detalles Bibliográficos
Autores principales: Yang, Qing, Meyerson, Nicholas R., Clark, Stephen K., Paige, Camille L., Fattor, Will T., Gilchrist, Alison R., Barbachano-Guerrero, Arturo, Healy, Benjamin G., Worden-Sapper, Emma R., Wu, Sharon S., Muhlrad, Denise, Decker, Carolyn J., Saldi, Tassa K., Lasda, Erika, Gonzales, Patrick K., Fink, Morgan R., Tat, Kimngan L., Hager, Cole R., Davis, Jack C., Ozeroff, Christopher D., Brisson, Gloria R., McQueen, Matthew B., Leinwand, Leslie, Parker, Roy, Sawyer, Sara L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7899473/
https://www.ncbi.nlm.nih.gov/pubmed/33619503
http://dx.doi.org/10.1101/2020.07.16.20150250

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