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Graphene oxide assisted light-up aptamer selection against Thioflavin T for label-free detection of microRNA

We selected an aptamer against a fluorogenic dye called Thioflavin T (ThT). Aptamers are single-stranded DNA that can bind a specific target. We selected the ThT aptamer using graphene oxide assisted SELEX and a low-cost Open qPCR instrument. We optimized, minimized, and characterized the best aptam...

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Detalles Bibliográficos
Autores principales: Islam, Md Mamunul, Ghielmetti, Victoria Michele, Allen, Peter B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900183/
https://www.ncbi.nlm.nih.gov/pubmed/33619372
http://dx.doi.org/10.1038/s41598-021-83640-z