Cargando…

Graphene oxide assisted light-up aptamer selection against Thioflavin T for label-free detection of microRNA

We selected an aptamer against a fluorogenic dye called Thioflavin T (ThT). Aptamers are single-stranded DNA that can bind a specific target. We selected the ThT aptamer using graphene oxide assisted SELEX and a low-cost Open qPCR instrument. We optimized, minimized, and characterized the best aptam...

Descripción completa

Detalles Bibliográficos
Autores principales:	Islam, Md Mamunul, Ghielmetti, Victoria Michele, Allen, Peter B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900183/ https://www.ncbi.nlm.nih.gov/pubmed/33619372 http://dx.doi.org/10.1038/s41598-021-83640-z

Ejemplares similares

Label-Free G-Quadruplex Aptamer Fluorescence Assay for Ochratoxin A Using a Thioflavin T Probe
por: Wu, Kefeng, et al.
Publicado: (2018)

A Label-Free Fluorescence Aptasensor Based on G-Quadruplex/Thioflavin T Complex for the Detection of Trypsin
por: Gu, Pan, et al.
Publicado: (2022)

Label-Free Fluorescence Molecular Beacon Probes Based on G-Triplex DNA and Thioflavin T for Protein Detection
por: Xue, Jun, et al.
Publicado: (2021)

Thioflavin T as a fluorescence light-up probe for G4 formation
por: Renaud de la Faverie, Amandine, et al.
Publicado: (2014)

Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays
por: Zhang, Hanxiao, et al.
Publicado: (2023)

A label-free aptamer-based biosensor for microRNA detection by the RNA-regulated fluorescence of malachite green
por: Wang, Honghong, et al.
Publicado: (2019)

Role of microRNA and Oxidative Stress in Influenza A Virus Pathogenesis
por: Haque, Md Mamunul, et al.
Publicado: (2020)

A Sensitive and Label-Free Pb(II) Fluorescence Sensor Based on a DNAzyme Controlled G-Quadruplex/Thioflavin T Conformation
por: Wen, Yanli, et al.
Publicado: (2016)

One-Step Photochemical Immobilization of Aptamer on Graphene for Label-Free Detection of NT-proBNP
por: Nekrasov, Nikita, et al.
Publicado: (2022)

Label-Free MicroRNA Optical Biosensors
por: Lai, Meimei, et al.
Publicado: (2019)

Telomere G-triplex lights up Thioflavin T for RNA detection: new wine in an old bottle
por: Qin, Shanshan, et al.
Publicado: (2022)

Label-Free Aptamer-Based Biosensor for Specific Detection of Chloramphenicol Using AIE Probe and Graphene Oxide
por: Zhang, Song, et al.
Publicado: (2018)

Inhalable Thioflavin S for the Detection of Amyloid Beta Deposits in the Retina
por: Barton, Shawn M., et al.
Publicado: (2021)

Thioflavin T indicates mitochondrial membrane potential in mammalian cells
por: Skates, Emily, et al.
Publicado: (2023)

Label-Free Quantification of MicroRNAs Using Ligase-Assisted Sandwich Hybridization on a DNA Microarray
por: Ueno, Taro, et al.
Publicado: (2014)

Rapid and label-free fluorescence bioassay for microRNA based on exonuclease III-assisted cycle amplification
por: Liu, Ming Xiu, et al.
Publicado: (2018)

Simple, low-cost fabrication of acrylic based droplet microfluidics and its use to generate DNA-coated particles
por: Islam, Md. Mamunul, et al.
Publicado: (2018)

Identifying Insulin Fibril Conformational Differences by Thioflavin-T Binding Characteristics
por: Ziaunys, Mantas, et al.
Publicado: (2020)

Exploring the occurrence of thioflavin-T-positive insulin amyloid aggregation intermediates
por: Ziaunys, Mantas, et al.
Publicado: (2021)

Label-Free Homogeneous microRNA Detection in Cell Culture Medium Based on Graphene Oxide and Specific Fluorescence Quenching
por: Nitu, Florentin R., et al.
Publicado: (2021)

Capture-SELEX for Chloramphenicol Binding Aptamers for Labeled and Label-Free Fluorescence Sensing
por: Zhao, Yichen, et al.
Publicado: (2023)

In Vivo Evaluation of the Nitroimidazole-Based Thioflavin-T Derivatives as Cerebral Ischemia Markers
por: Chu, Taiwei, et al.
Publicado: (2007)

Analyzing Thioflavin T Binding to Amyloid Fibrils by an Equilibrium Microdialysis-Based Technique
por: Kuznetsova, Irina M., et al.
Publicado: (2012)

Thioflavin T as an efficient fluorescence sensor for selective recognition of RNA G-quadruplexes
por: Xu, Shujuan, et al.
Publicado: (2016)

Investigation of α-Synuclein Amyloid Fibrils Using the Fluorescent Probe Thioflavin T
por: Sulatskaya, Anna I., et al.
Publicado: (2018)

Thioflavin T as an amyloid dye: fibril quantification, optimal concentration and effect on aggregation
por: Xue, Christine, et al.
Publicado: (2017)

Stoichiometry and Affinity of Thioflavin T Binding to Sup35p Amyloid Fibrils
por: Sulatskaya, Anna I., et al.
Publicado: (2016)

Laser Emission of Thioflavin T Uncovers Protein Aggregation in Amyloid Nucleation Phase
por: Hanczyc, Piotr, et al.
Publicado: (2021)

Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses
por: Islam, Md Mamunul, et al.
Publicado: (2022)

Aptamer Functionalized Lipid Multilayer Gratings for Label-Free Analyte Detection
por: Prommapan, Plengchart, et al.
Publicado: (2020)

Detection of Oxytetracycline Using an Electrochemical Label-Free Aptamer-Based Biosensor
por: Akbarzadeh, Sanaz, et al.
Publicado: (2022)

Fluorescence Quantum Yield of Thioflavin T in Rigid Isotropic Solution and Incorporated into the Amyloid Fibrils
por: Sulatskaya, Anna I., et al.
Publicado: (2010)

Thioflavin T as a fluorescence probe for monitoring RNA metabolism at molecular and cellular levels
por: Sugimoto, Shinya, et al.
Publicado: (2015)

Polarized super-resolution structural imaging inside amyloid fibrils using Thioflavine T
por: Shaban, Haitham A., et al.
Publicado: (2017)

Molecular recognition of human islet amyloid polypeptide assembly by selective oligomerization of thioflavin T
por: Yu, Lanlan, et al.
Publicado: (2020)

Effect of Ionic Strength on Thioflavin-T Affinity to Amyloid Fibrils and Its Fluorescence Intensity
por: Mikalauskaite, Kamile, et al.
Publicado: (2020)

Biochar from Wood Chips and Corn Cobs for Adsorption of Thioflavin T and Erythrosine B
por: Pipíška, Martin, et al.
Publicado: (2022)

Quantitation of Tissue Amyloid via Fluorescence Spectroscopy Using Controlled Concentrations of Thioflavin-S
por: MacKeigan, Tatiana P., et al.
Publicado: (2023)

Thioflavin-T does not report on electrochemical potential and memory of dormant or germinating bacterial spores
por: Li, Yong-qing, et al.
Publicado: (2023)

Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers
por: Jolly, Pawan, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_jgmipanpqor0kf3g40v2d053t2