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Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato

DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein (RNP) transfection of potato protoplasts, a mutation frequency up to 72%...

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Detalles Bibliográficos
Autores principales:	Zhao, Xue, Jayarathna, Shishanthi, Turesson, Helle, Fält, Ann-Sofie, Nestor, Gustav, González, Matías N., Olsson, Niklas, Beganovic, Mirela, Hofvander, Per, Andersson, Roger, Andersson, Mariette
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900246/ https://www.ncbi.nlm.nih.gov/pubmed/33619312 http://dx.doi.org/10.1038/s41598-021-83462-z

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