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High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
Antibodies represent powerful tools to examine signal transduction pathways. Here, we present a strategy integrating multiple state-of-the-art methods to produce, validate, and utilize antibodies. Focusing on understudied synaptic proteins, we generated 137 recombinant antibodies. We used yeast disp...
Autores principales: | Lemos Duarte, Mariana, Trimbake, Nikita A., Gupta, Achla, Tumanut, Christine, Fan, Xiaomin, Woods, Catherine, Ram, Akila, Gomes, Ivone, Bobeck, Erin N., Schechtman, Deborah, Devi, Lakshmi A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900253/ https://www.ncbi.nlm.nih.gov/pubmed/33619305 http://dx.doi.org/10.1038/s42003-021-01744-8 |
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