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High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics

Antibodies represent powerful tools to examine signal transduction pathways. Here, we present a strategy integrating multiple state-of-the-art methods to produce, validate, and utilize antibodies. Focusing on understudied synaptic proteins, we generated 137 recombinant antibodies. We used yeast disp...

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Detalles Bibliográficos
Autores principales: Lemos Duarte, Mariana, Trimbake, Nikita A., Gupta, Achla, Tumanut, Christine, Fan, Xiaomin, Woods, Catherine, Ram, Akila, Gomes, Ivone, Bobeck, Erin N., Schechtman, Deborah, Devi, Lakshmi A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900253/
https://www.ncbi.nlm.nih.gov/pubmed/33619305
http://dx.doi.org/10.1038/s42003-021-01744-8

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