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Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer

Frozen and thawed embryo transfer (FET) is currently widely applied in routine assisted reproductive technology (ART) procedure. It is of great necessity to assess the safety of FET and investigate the long-term effect including glucose metabolism on FET-conceived offspring. The mouse model is a hig...

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Autores principales: Qin, Ningxin, Zhou, Zhiyang, Zhao, Wenlong, Zou, Kexin, Shi, Weihui, Yu, Chuanjin, Liu, Xia, Dong, Zehan, Mao, Yiting, Liu, Xinmei, Sheng, Jianzhong, Ding, Guolian, Wu, Yanting, Huang, Hefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900417/
https://www.ncbi.nlm.nih.gov/pubmed/33634140
http://dx.doi.org/10.3389/fcell.2021.637781
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author Qin, Ningxin
Zhou, Zhiyang
Zhao, Wenlong
Zou, Kexin
Shi, Weihui
Yu, Chuanjin
Liu, Xia
Dong, Zehan
Mao, Yiting
Liu, Xinmei
Sheng, Jianzhong
Ding, Guolian
Wu, Yanting
Huang, Hefeng
author_facet Qin, Ningxin
Zhou, Zhiyang
Zhao, Wenlong
Zou, Kexin
Shi, Weihui
Yu, Chuanjin
Liu, Xia
Dong, Zehan
Mao, Yiting
Liu, Xinmei
Sheng, Jianzhong
Ding, Guolian
Wu, Yanting
Huang, Hefeng
author_sort Qin, Ningxin
collection PubMed
description Frozen and thawed embryo transfer (FET) is currently widely applied in routine assisted reproductive technology (ART) procedure. It is of great necessity to assess the safety of FET and investigate the long-term effect including glucose metabolism on FET-conceived offspring. The mouse model is a highly efficient method to figure out the relationship between the process of FET and offspring health. In this study, we obtained mouse offspring of natural conception (NC), in vitro fertilization (IVF), and FET. Glucose and insulin tolerance test (GTT/ITT) were performed on both chow fed or high fat diet (HFD) fed offspring to examine the glucose metabolism status. We detected hepatic PI3K/AKT pathway by western blotting and transcriptome status by RNA-sequencing. Impaired glucose tolerance (IGT) and decreased insulin tolerance were occurred in FET conceived male offspring. After challenged with the HFD-fed, male offspring in FET group performed earlier and severer IGT than IVF group. Furthermore, higher HOMA-IR index and higher serum insulin level post glucose injected in FET-chow group suggested the insulin resistance status. The PI3K/AKT signaling pathway, the major pathway of insulin in the liver, were also disrupted in FET group. Transcriptomics of the liver reveals significantly downregulated in glucose metabolic process and insulin resistance in the FET-chow group. In our study, FET-conceived male mouse offspring presented glucose metabolism dysfunction mainly manifesting insulin resistance. The hepatic insulin signaling pathway were in concordance with reduced glycogen synthesis, increased glycolysis and enhanced gluconeogenesis status in FET-conceived male offspring.
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spelling pubmed-79004172021-02-24 Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer Qin, Ningxin Zhou, Zhiyang Zhao, Wenlong Zou, Kexin Shi, Weihui Yu, Chuanjin Liu, Xia Dong, Zehan Mao, Yiting Liu, Xinmei Sheng, Jianzhong Ding, Guolian Wu, Yanting Huang, Hefeng Front Cell Dev Biol Cell and Developmental Biology Frozen and thawed embryo transfer (FET) is currently widely applied in routine assisted reproductive technology (ART) procedure. It is of great necessity to assess the safety of FET and investigate the long-term effect including glucose metabolism on FET-conceived offspring. The mouse model is a highly efficient method to figure out the relationship between the process of FET and offspring health. In this study, we obtained mouse offspring of natural conception (NC), in vitro fertilization (IVF), and FET. Glucose and insulin tolerance test (GTT/ITT) were performed on both chow fed or high fat diet (HFD) fed offspring to examine the glucose metabolism status. We detected hepatic PI3K/AKT pathway by western blotting and transcriptome status by RNA-sequencing. Impaired glucose tolerance (IGT) and decreased insulin tolerance were occurred in FET conceived male offspring. After challenged with the HFD-fed, male offspring in FET group performed earlier and severer IGT than IVF group. Furthermore, higher HOMA-IR index and higher serum insulin level post glucose injected in FET-chow group suggested the insulin resistance status. The PI3K/AKT signaling pathway, the major pathway of insulin in the liver, were also disrupted in FET group. Transcriptomics of the liver reveals significantly downregulated in glucose metabolic process and insulin resistance in the FET-chow group. In our study, FET-conceived male mouse offspring presented glucose metabolism dysfunction mainly manifesting insulin resistance. The hepatic insulin signaling pathway were in concordance with reduced glycogen synthesis, increased glycolysis and enhanced gluconeogenesis status in FET-conceived male offspring. Frontiers Media S.A. 2021-02-09 /pmc/articles/PMC7900417/ /pubmed/33634140 http://dx.doi.org/10.3389/fcell.2021.637781 Text en Copyright © 2021 Qin, Zhou, Zhao, Zou, Shi, Yu, Liu, Dong, Mao, Liu, Sheng, Ding, Wu and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Qin, Ningxin
Zhou, Zhiyang
Zhao, Wenlong
Zou, Kexin
Shi, Weihui
Yu, Chuanjin
Liu, Xia
Dong, Zehan
Mao, Yiting
Liu, Xinmei
Sheng, Jianzhong
Ding, Guolian
Wu, Yanting
Huang, Hefeng
Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer
title Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer
title_full Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer
title_fullStr Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer
title_full_unstemmed Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer
title_short Abnormal Glucose Metabolism in Male Mice Offspring Conceived by in vitro Fertilization and Frozen-Thawed Embryo Transfer
title_sort abnormal glucose metabolism in male mice offspring conceived by in vitro fertilization and frozen-thawed embryo transfer
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900417/
https://www.ncbi.nlm.nih.gov/pubmed/33634140
http://dx.doi.org/10.3389/fcell.2021.637781
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