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Evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: A single-center study

BACKGROUND: ISPD recommends culture-negative peritonitis in each peritoneal dialysis (PD) center should less than 15%. The hospital in Thailand, however faced a significantly high rate of culture negative peritonitis, even using blood culture bottles technique. This study evaluates the performance o...

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Autores principales: Tanratananon, Duangrat, Deekae, Soawarat, Raksasuk, Sukit, Srithongkul, Thatsaphan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900635/
https://www.ncbi.nlm.nih.gov/pubmed/33664942
http://dx.doi.org/10.1016/j.amsu.2021.01.087
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author Tanratananon, Duangrat
Deekae, Soawarat
Raksasuk, Sukit
Srithongkul, Thatsaphan
author_facet Tanratananon, Duangrat
Deekae, Soawarat
Raksasuk, Sukit
Srithongkul, Thatsaphan
author_sort Tanratananon, Duangrat
collection PubMed
description BACKGROUND: ISPD recommends culture-negative peritonitis in each peritoneal dialysis (PD) center should less than 15%. The hospital in Thailand, however faced a significantly high rate of culture negative peritonitis, even using blood culture bottles technique. This study evaluates the performance of three different culture methods in detecting organisms in PD related peritonitis. METHODS: A prospective cohort diagnostic study was performed in PD patients diagnosed with PD related peritonitis in Surin Hospital from October 2018 to June 2020. The Diagnosis of peritonitis was followed ISPD guidelines. PDF sample from each patient was processed by three different blood culture bottle-based techniques, including i) 50 ml PDF centrifugation, and ii) 10 ml PDF centrifugation before inoculated into blood culture bottles, and iii) inoculation into blood culture bottles without centrifugation. The sensitivities and isolated organisms were compared among the individual methods. RESULTS: Of 126 PD patients with clinical peritonitis, PD related peritonitis was diagnosed in 87 patients with 105 PDF analysis. PDF culture showed gram-positive organisms 34%, gram-negative organisms 41%, fungal 5.71%, and culture-negative result in 22.86%. The direct blood culture method was positive in 59.05%, while centrifugation before inoculated into blood culture bottles, has a higher percentage of positive results, 60.95% and 64.76% from 10 ml to 50 ml PDF volume; respectively. The sensitivity was 84% and 76.5% for 50 ml PDF centrifugation and blood culture without centrifugation. CONCLUSION: Large volume PDF centrifugation before inoculating into blood culture bottles may improve the positive culture rate in PD related peritonitis.
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spelling pubmed-79006352021-03-03 Evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: A single-center study Tanratananon, Duangrat Deekae, Soawarat Raksasuk, Sukit Srithongkul, Thatsaphan Ann Med Surg (Lond) Diagnostic Study BACKGROUND: ISPD recommends culture-negative peritonitis in each peritoneal dialysis (PD) center should less than 15%. The hospital in Thailand, however faced a significantly high rate of culture negative peritonitis, even using blood culture bottles technique. This study evaluates the performance of three different culture methods in detecting organisms in PD related peritonitis. METHODS: A prospective cohort diagnostic study was performed in PD patients diagnosed with PD related peritonitis in Surin Hospital from October 2018 to June 2020. The Diagnosis of peritonitis was followed ISPD guidelines. PDF sample from each patient was processed by three different blood culture bottle-based techniques, including i) 50 ml PDF centrifugation, and ii) 10 ml PDF centrifugation before inoculated into blood culture bottles, and iii) inoculation into blood culture bottles without centrifugation. The sensitivities and isolated organisms were compared among the individual methods. RESULTS: Of 126 PD patients with clinical peritonitis, PD related peritonitis was diagnosed in 87 patients with 105 PDF analysis. PDF culture showed gram-positive organisms 34%, gram-negative organisms 41%, fungal 5.71%, and culture-negative result in 22.86%. The direct blood culture method was positive in 59.05%, while centrifugation before inoculated into blood culture bottles, has a higher percentage of positive results, 60.95% and 64.76% from 10 ml to 50 ml PDF volume; respectively. The sensitivity was 84% and 76.5% for 50 ml PDF centrifugation and blood culture without centrifugation. CONCLUSION: Large volume PDF centrifugation before inoculating into blood culture bottles may improve the positive culture rate in PD related peritonitis. Elsevier 2021-02-13 /pmc/articles/PMC7900635/ /pubmed/33664942 http://dx.doi.org/10.1016/j.amsu.2021.01.087 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Diagnostic Study
Tanratananon, Duangrat
Deekae, Soawarat
Raksasuk, Sukit
Srithongkul, Thatsaphan
Evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: A single-center study
title Evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: A single-center study
title_full Evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: A single-center study
title_fullStr Evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: A single-center study
title_full_unstemmed Evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: A single-center study
title_short Evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: A single-center study
title_sort evaluation of different methods to improve culture-negative peritoneal dialysis-related peritonitis: a single-center study
topic Diagnostic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900635/
https://www.ncbi.nlm.nih.gov/pubmed/33664942
http://dx.doi.org/10.1016/j.amsu.2021.01.087
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