C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane

C. elegans spe-9 class genes encode sperm proteins with indispensable roles during fertilization. We have previously reported that spe-45 belongs to the spe-9 class, based on the finding that self-sperm of spe-45(tm3715) hermaphrodites were not consumed by fertilization. In this study, we directly o...

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Detalles Bibliográficos
Autores principales: Takayama, Jun, Tajima, Tatsuya, Onami, Shuichi, Nishimura, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900825/
https://www.ncbi.nlm.nih.gov/pubmed/33644705
http://dx.doi.org/10.17912/micropub.biology.000372
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author Takayama, Jun
Tajima, Tatsuya
Onami, Shuichi
Nishimura, Hitoshi
author_facet Takayama, Jun
Tajima, Tatsuya
Onami, Shuichi
Nishimura, Hitoshi
author_sort Takayama, Jun
collection PubMed
description C. elegans spe-9 class genes encode sperm proteins with indispensable roles during fertilization. We have previously reported that spe-45 belongs to the spe-9 class, based on the finding that self-sperm of spe-45(tm3715) hermaphrodites were not consumed by fertilization. In this study, we directly observed live fertilization in the spermatheca of fem-1(hc17) females after mating with spe-45(tm3715) males. As expected, it was clearly shown that spe-45 mutant spermatozoa failed to fuse with the oocyte plasma membrane. Thus, our live imaging system for C. elegans fertilization seems to be useful for evaluation of the functions of male and female gametes.
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spelling pubmed-79008252021-02-26 C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane Takayama, Jun Tajima, Tatsuya Onami, Shuichi Nishimura, Hitoshi MicroPubl Biol New Finding C. elegans spe-9 class genes encode sperm proteins with indispensable roles during fertilization. We have previously reported that spe-45 belongs to the spe-9 class, based on the finding that self-sperm of spe-45(tm3715) hermaphrodites were not consumed by fertilization. In this study, we directly observed live fertilization in the spermatheca of fem-1(hc17) females after mating with spe-45(tm3715) males. As expected, it was clearly shown that spe-45 mutant spermatozoa failed to fuse with the oocyte plasma membrane. Thus, our live imaging system for C. elegans fertilization seems to be useful for evaluation of the functions of male and female gametes. Caltech Library 2021-02-21 /pmc/articles/PMC7900825/ /pubmed/33644705 http://dx.doi.org/10.17912/micropub.biology.000372 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Takayama, Jun
Tajima, Tatsuya
Onami, Shuichi
Nishimura, Hitoshi
C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane
title C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane
title_full C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane
title_fullStr C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane
title_full_unstemmed C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane
title_short C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane
title_sort c. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900825/
https://www.ncbi.nlm.nih.gov/pubmed/33644705
http://dx.doi.org/10.17912/micropub.biology.000372
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