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C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane
C. elegans spe-9 class genes encode sperm proteins with indispensable roles during fertilization. We have previously reported that spe-45 belongs to the spe-9 class, based on the finding that self-sperm of spe-45(tm3715) hermaphrodites were not consumed by fertilization. In this study, we directly o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900825/ https://www.ncbi.nlm.nih.gov/pubmed/33644705 http://dx.doi.org/10.17912/micropub.biology.000372 |
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author | Takayama, Jun Tajima, Tatsuya Onami, Shuichi Nishimura, Hitoshi |
author_facet | Takayama, Jun Tajima, Tatsuya Onami, Shuichi Nishimura, Hitoshi |
author_sort | Takayama, Jun |
collection | PubMed |
description | C. elegans spe-9 class genes encode sperm proteins with indispensable roles during fertilization. We have previously reported that spe-45 belongs to the spe-9 class, based on the finding that self-sperm of spe-45(tm3715) hermaphrodites were not consumed by fertilization. In this study, we directly observed live fertilization in the spermatheca of fem-1(hc17) females after mating with spe-45(tm3715) males. As expected, it was clearly shown that spe-45 mutant spermatozoa failed to fuse with the oocyte plasma membrane. Thus, our live imaging system for C. elegans fertilization seems to be useful for evaluation of the functions of male and female gametes. |
format | Online Article Text |
id | pubmed-7900825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-79008252021-02-26 C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane Takayama, Jun Tajima, Tatsuya Onami, Shuichi Nishimura, Hitoshi MicroPubl Biol New Finding C. elegans spe-9 class genes encode sperm proteins with indispensable roles during fertilization. We have previously reported that spe-45 belongs to the spe-9 class, based on the finding that self-sperm of spe-45(tm3715) hermaphrodites were not consumed by fertilization. In this study, we directly observed live fertilization in the spermatheca of fem-1(hc17) females after mating with spe-45(tm3715) males. As expected, it was clearly shown that spe-45 mutant spermatozoa failed to fuse with the oocyte plasma membrane. Thus, our live imaging system for C. elegans fertilization seems to be useful for evaluation of the functions of male and female gametes. Caltech Library 2021-02-21 /pmc/articles/PMC7900825/ /pubmed/33644705 http://dx.doi.org/10.17912/micropub.biology.000372 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Takayama, Jun Tajima, Tatsuya Onami, Shuichi Nishimura, Hitoshi C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane |
title | C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane |
title_full | C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane |
title_fullStr | C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane |
title_full_unstemmed | C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane |
title_short | C. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane |
title_sort | c. elegans spermatozoa lacking spe-45 are incapable of fusing with the oocyte plasma membrane |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900825/ https://www.ncbi.nlm.nih.gov/pubmed/33644705 http://dx.doi.org/10.17912/micropub.biology.000372 |
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