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Cognate base‐pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase

We present cognate base pair selectivity in template‐dependent ligation by T4 DNA ligase using a hydrophobic unnatural base pair (UBP), Ds‐Pa. T4 DNA ligase efficiently recognizes the Ds‐Pa pairing at the conjugation position, and Ds excludes the noncognate pairings with the natural bases. Our resul...

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Detalles Bibliográficos
Autores principales:	Kimoto, Michiko, Soh, Si Hui Gabriella, Tan, Hui Pen, Okamoto, Itaru, Hirao, Ichiro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley & Sons, Inc. 2020
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900958/ https://www.ncbi.nlm.nih.gov/pubmed/33156531 http://dx.doi.org/10.1002/bip.23407

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