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Reliable measurements of extracellular vesicles by clinical flow cytometry

Extracellular vesicles (EVs) are cell‐derived particles with a phospholipid membrane present in all body fluids. Because EV properties change in health and disease, EVs have excellent potential to become biomarkers for diagnosis, prognosis, or monitoring of disease. The only technique capable of det...

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Autores principales: Kuiper, Martine, van de Nes, Arthur, Nieuwland, Rienk, Varga, Zoltan, van der Pol, Edwin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900981/
https://www.ncbi.nlm.nih.gov/pubmed/32966654
http://dx.doi.org/10.1111/aji.13350
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author Kuiper, Martine
van de Nes, Arthur
Nieuwland, Rienk
Varga, Zoltan
van der Pol, Edwin
author_facet Kuiper, Martine
van de Nes, Arthur
Nieuwland, Rienk
Varga, Zoltan
van der Pol, Edwin
author_sort Kuiper, Martine
collection PubMed
description Extracellular vesicles (EVs) are cell‐derived particles with a phospholipid membrane present in all body fluids. Because EV properties change in health and disease, EVs have excellent potential to become biomarkers for diagnosis, prognosis, or monitoring of disease. The only technique capable of detecting, sizing, and phenotyping a million of EVs within minutes is (clinical) flow cytometry. A flow cytometer measures light scattering and fluorescence signals of single EVs. Although these signals contain valuable information about the presence and composition of EVs, the signals are expressed in arbitrary units, which make the comparison of measurement results impossible between instruments and laboratories. Additionally, unintended and undocumented variations in the source, preparation, and analysis of the sample lead to orders of magnitude variations in the measured EV concentrations. Here, we will explain the basics, challenges, and common misconceptions of EV flow cytometry. In addition, we provide an overview of recent standardization initiatives, which are a prerequisite for comparison of clinical data and thus for clinical biomarker exploration of EVs.
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spelling pubmed-79009812021-03-03 Reliable measurements of extracellular vesicles by clinical flow cytometry Kuiper, Martine van de Nes, Arthur Nieuwland, Rienk Varga, Zoltan van der Pol, Edwin Am J Reprod Immunol Special Issue Articles Extracellular vesicles (EVs) are cell‐derived particles with a phospholipid membrane present in all body fluids. Because EV properties change in health and disease, EVs have excellent potential to become biomarkers for diagnosis, prognosis, or monitoring of disease. The only technique capable of detecting, sizing, and phenotyping a million of EVs within minutes is (clinical) flow cytometry. A flow cytometer measures light scattering and fluorescence signals of single EVs. Although these signals contain valuable information about the presence and composition of EVs, the signals are expressed in arbitrary units, which make the comparison of measurement results impossible between instruments and laboratories. Additionally, unintended and undocumented variations in the source, preparation, and analysis of the sample lead to orders of magnitude variations in the measured EV concentrations. Here, we will explain the basics, challenges, and common misconceptions of EV flow cytometry. In addition, we provide an overview of recent standardization initiatives, which are a prerequisite for comparison of clinical data and thus for clinical biomarker exploration of EVs. John Wiley and Sons Inc. 2020-10-08 2021-02 /pmc/articles/PMC7900981/ /pubmed/32966654 http://dx.doi.org/10.1111/aji.13350 Text en © 2020 The Authors. American Journal of Reproductive Immunology published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Special Issue Articles
Kuiper, Martine
van de Nes, Arthur
Nieuwland, Rienk
Varga, Zoltan
van der Pol, Edwin
Reliable measurements of extracellular vesicles by clinical flow cytometry
title Reliable measurements of extracellular vesicles by clinical flow cytometry
title_full Reliable measurements of extracellular vesicles by clinical flow cytometry
title_fullStr Reliable measurements of extracellular vesicles by clinical flow cytometry
title_full_unstemmed Reliable measurements of extracellular vesicles by clinical flow cytometry
title_short Reliable measurements of extracellular vesicles by clinical flow cytometry
title_sort reliable measurements of extracellular vesicles by clinical flow cytometry
topic Special Issue Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900981/
https://www.ncbi.nlm.nih.gov/pubmed/32966654
http://dx.doi.org/10.1111/aji.13350
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