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Xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of Cajal in mice

BACKGROUND: Interdigestive migrating motor complexes (MMC) produce periodic contractions in the gastrointestinal tract, but the exact mechanism of action still remains unclear. Intramuscular interstitial cells of Cajal (ICC-IM) participate in gastrointestinal hormone and neuromodulation, but the cor...

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Autores principales: Chen, Qi-Cheng, Jiang, Zhi, Zhang, Jun-Hong, Cao, Li-Xing, Chen, Zhi-Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901053/
https://www.ncbi.nlm.nih.gov/pubmed/33642830
http://dx.doi.org/10.3748/wjg.v27.i7.576
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author Chen, Qi-Cheng
Jiang, Zhi
Zhang, Jun-Hong
Cao, Li-Xing
Chen, Zhi-Qiang
author_facet Chen, Qi-Cheng
Jiang, Zhi
Zhang, Jun-Hong
Cao, Li-Xing
Chen, Zhi-Qiang
author_sort Chen, Qi-Cheng
collection PubMed
description BACKGROUND: Interdigestive migrating motor complexes (MMC) produce periodic contractions in the gastrointestinal tract, but the exact mechanism of action still remains unclear. Intramuscular interstitial cells of Cajal (ICC-IM) participate in gastrointestinal hormone and neuromodulation, but the correlation between ICC-IM and MMC is also unclear. We found that xiangbinfang granules (XBF) mediated the phase III contraction of MMC. Here, the effects of XBF on gastric antrum motility in W/W(v) mice and the effects of ICC-IM on gastric antrum MMC are reported. AIM: To observe the effects of ICC-IM on gastric antrum motility and to establish the mechanism of XBF in promoting gastric antrum motility. METHODS: The density of c-kit-positive ICC myenteric plexus (ICC-MP) and ICC-IM in the antral muscularis of W/W(v) and wild-type (WT) mice was examined by confocal microscopy. The effects of XBF on gastric antrum slow waves in W/W(v) and WT mice were recorded by intracellular amplification recording. Micro-strain-gauge force transducers were implanted into the gastric antrum to monitor the MMC and the effect of XBF on gastric antrum motility in conscious W/W(v) and WT mice. RESULTS: In the gastric antrum of W/W(v) mice, c-kit immunoreactivity was significantly reduced, and no ICC-IM network was observed. Spontaneous rhythmic slow waves also appeared in the antrum of W/W(v) mice, but the amplitude of the antrum slow wave decreased significantly in W/W(v) mice (22.62 ± 2.23 mV vs 2.92 ± 0.52 mV, P < 0.0001). MMCs were found in 7 of the 8 WT mice but no complete MMC cycle was found in W/W(v) mice. The contractile frequency and amplitude index of the gastric antrum were significantly increased in conscious WT compared to W/W(v) mice (frequency, 3.53 ± 0.18 cpm vs 1.28 ± 0.12 cpm; amplitude index, 23014.26 ± 1798.65 mV·20 min vs 3782.16 ± 407.13 mV·20 min; P < 0.0001). XBF depolarized smooth muscle cells of the gastric antrum in WT and W/W(v) mice in a dose-dependent manner. Similarly, the gastric antrum motility in WT mice was significantly increased after treatment with XBF 5 mg (P < 0.05). Atropine (0.1 mg/kg) blocked the enhancement of XBF in WT and W/W(v) mice completely, while tetrodotoxin (0.05 mg/kg) partially inhibited the enhancement by XBF. CONCLUSION: ICC-IM participates in the regulation of gastric antrum MMC in mice. XBF induces MMC III-like contractions that enhance gastric antrum motility via ICC-IM in mice.
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spelling pubmed-79010532021-02-26 Xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of Cajal in mice Chen, Qi-Cheng Jiang, Zhi Zhang, Jun-Hong Cao, Li-Xing Chen, Zhi-Qiang World J Gastroenterol Basic Study BACKGROUND: Interdigestive migrating motor complexes (MMC) produce periodic contractions in the gastrointestinal tract, but the exact mechanism of action still remains unclear. Intramuscular interstitial cells of Cajal (ICC-IM) participate in gastrointestinal hormone and neuromodulation, but the correlation between ICC-IM and MMC is also unclear. We found that xiangbinfang granules (XBF) mediated the phase III contraction of MMC. Here, the effects of XBF on gastric antrum motility in W/W(v) mice and the effects of ICC-IM on gastric antrum MMC are reported. AIM: To observe the effects of ICC-IM on gastric antrum motility and to establish the mechanism of XBF in promoting gastric antrum motility. METHODS: The density of c-kit-positive ICC myenteric plexus (ICC-MP) and ICC-IM in the antral muscularis of W/W(v) and wild-type (WT) mice was examined by confocal microscopy. The effects of XBF on gastric antrum slow waves in W/W(v) and WT mice were recorded by intracellular amplification recording. Micro-strain-gauge force transducers were implanted into the gastric antrum to monitor the MMC and the effect of XBF on gastric antrum motility in conscious W/W(v) and WT mice. RESULTS: In the gastric antrum of W/W(v) mice, c-kit immunoreactivity was significantly reduced, and no ICC-IM network was observed. Spontaneous rhythmic slow waves also appeared in the antrum of W/W(v) mice, but the amplitude of the antrum slow wave decreased significantly in W/W(v) mice (22.62 ± 2.23 mV vs 2.92 ± 0.52 mV, P < 0.0001). MMCs were found in 7 of the 8 WT mice but no complete MMC cycle was found in W/W(v) mice. The contractile frequency and amplitude index of the gastric antrum were significantly increased in conscious WT compared to W/W(v) mice (frequency, 3.53 ± 0.18 cpm vs 1.28 ± 0.12 cpm; amplitude index, 23014.26 ± 1798.65 mV·20 min vs 3782.16 ± 407.13 mV·20 min; P < 0.0001). XBF depolarized smooth muscle cells of the gastric antrum in WT and W/W(v) mice in a dose-dependent manner. Similarly, the gastric antrum motility in WT mice was significantly increased after treatment with XBF 5 mg (P < 0.05). Atropine (0.1 mg/kg) blocked the enhancement of XBF in WT and W/W(v) mice completely, while tetrodotoxin (0.05 mg/kg) partially inhibited the enhancement by XBF. CONCLUSION: ICC-IM participates in the regulation of gastric antrum MMC in mice. XBF induces MMC III-like contractions that enhance gastric antrum motility via ICC-IM in mice. Baishideng Publishing Group Inc 2021-02-21 2021-02-21 /pmc/articles/PMC7901053/ /pubmed/33642830 http://dx.doi.org/10.3748/wjg.v27.i7.576 Text en ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
spellingShingle Basic Study
Chen, Qi-Cheng
Jiang, Zhi
Zhang, Jun-Hong
Cao, Li-Xing
Chen, Zhi-Qiang
Xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of Cajal in mice
title Xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of Cajal in mice
title_full Xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of Cajal in mice
title_fullStr Xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of Cajal in mice
title_full_unstemmed Xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of Cajal in mice
title_short Xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of Cajal in mice
title_sort xiangbinfang granules enhance gastric antrum motility via intramuscular interstitial cells of cajal in mice
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901053/
https://www.ncbi.nlm.nih.gov/pubmed/33642830
http://dx.doi.org/10.3748/wjg.v27.i7.576
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