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Gradient Internal Standard Method for Absolute Quantification of Microbial Amplicon Sequencing Data
High-throughput amplicon sequencing is a critical tool for studying microbiota; however, it results only in relative abundance data. Thus, changes in absolute abundance of microbiota cannot be determined, which hinders further microbiology research. We have therefore established a gradient internal...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901480/ https://www.ncbi.nlm.nih.gov/pubmed/33436513 http://dx.doi.org/10.1128/mSystems.00964-20 |