Cargando…

Gradient Internal Standard Method for Absolute Quantification of Microbial Amplicon Sequencing Data

High-throughput amplicon sequencing is a critical tool for studying microbiota; however, it results only in relative abundance data. Thus, changes in absolute abundance of microbiota cannot be determined, which hinders further microbiology research. We have therefore established a gradient internal...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Shilei, Wu, Qun, Han, Ying, Du, Rubing, Wang, Xiaoyong, Nie, Yao, Du, Xiaowei, Xu, Yan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901480/ https://www.ncbi.nlm.nih.gov/pubmed/33436513 http://dx.doi.org/10.1128/mSystems.00964-20

Ejemplares similares

Absolute quantification of proteins in the fatty acid biosynthetic pathway using protein standard absolute quantification
por: Tao, Hui, et al.
Publicado: (2016)

Median-Based Absolute Quantification of Proteins Using Fully Unlabeled Generic Internal Standard (FUGIS)
por: Raghuraman, Bharath Kumar, et al.
Publicado: (2021)

16S amplicon sequencing of microbial communities in enriched and non-enriched sediments of non-volcanic hot spring with temperature gradients
por: Yasir, Muhammad, et al.
Publicado: (2021)

Highly sensitive amplicon-based transcript quantification by semiconductor sequencing
por: Zhang, Jitao David, et al.
Publicado: (2014)

Comparison of the microbial composition of African fermented foods using amplicon sequencing
por: Diaz, Maria, et al.
Publicado: (2019)

Absolute quantification of microbial proteomes at different states by directed mass spectrometry
por: Schmidt, Alexander, et al.
Publicado: (2011)

AmpliconDuo: A Split-Sample Filtering Protocol for High-Throughput Amplicon Sequencing of Microbial Communities
por: Lange, Anja, et al.
Publicado: (2015)

SPINGO: a rapid species-classifier for microbial amplicon sequences
por: Allard, Guy, et al.
Publicado: (2015)

Ultra-accurate microbial amplicon sequencing with synthetic long reads
por: Callahan, Benjamin J., et al.
Publicado: (2021)

Internal Transcribed Spacer and 16S Amplicon Sequencing Identifies Microbial Species Associated with Asbestos in New Zealand
por: Doyle, Erin, et al.
Publicado: (2023)

Microbial dark matter sequences verification in amplicon sequencing and environmental metagenomics data
por: Barak, Hana, et al.
Publicado: (2023)

Amplicon sequencing for the quantification of spoilage microbiota in complex foods including bacterial spores
por: de Boer, Paulo, et al.
Publicado: (2015)

Predictive metabolomic profiling of microbial communities using amplicon or metagenomic sequences
por: Mallick, Himel, et al.
Publicado: (2019)

Comparing bioinformatic pipelines for microbial 16S rRNA amplicon sequencing
por: Prodan, Andrei, et al.
Publicado: (2020)

Microbial composition of Kombucha determined using amplicon sequencing and shotgun metagenomics
por: Arıkan, Muzaffer, et al.
Publicado: (2020)

Absolute Quantification of Human Liver Phosphorus-Containing Metabolites In Vivo Using an Inhomogeneous Spoiling Magnetic Field Gradient
por: Bashir, Adil, et al.
Publicado: (2015)

RNA-based amplicon sequencing is ineffective in measuring metabolic activity in environmental microbial communities
por: Wang, Ya, et al.
Publicado: (2023)

Absolute quantification of translational regulation and burden using combined sequencing approaches
por: Gorochowski, Thomas E, et al.
Publicado: (2019)

Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process
por: Smith, Marjo V, et al.
Publicado: (2007)

Phasing amplicon sequencing on Illumina Miseq for robust environmental microbial community analysis
por: Wu, Liyou, et al.
Publicado: (2015)

Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome
por: Tettamanti Boshier, Florencia A., et al.
Publicado: (2020)

Synthetic Sequencing Standards: A Guide to Database Choice for Rumen Microbiota Amplicon Sequencing Analysis
por: Smith, Paul E., et al.
Publicado: (2020)

FastCAT Accelerates Absolute Quantification of Proteins Using Multiple Short Nonpurified Chimeric Standards
por: Rzagalinski, Ignacy, et al.
Publicado: (2022)

Large-scale differences in microbial biodiversity discovery between 16S amplicon and shotgun sequencing
por: Tessler, Michael, et al.
Publicado: (2017)

Analysing Microbial Community Composition through Amplicon Sequencing: From Sampling to Hypothesis Testing
por: Hugerth, Luisa W., et al.
Publicado: (2017)

Rbec: a tool for analysis of amplicon sequencing data from synthetic microbial communities
por: Zhang, Pengfan, et al.
Publicado: (2021)

Effects of marine sediment as agricultural substrate on soil microbial diversity: an amplicon sequencing study
por: Núñez-Gómez, Dámaris, et al.
Publicado: (2023)

The Grapevine and Wine Microbiome: Insights from High-Throughput Amplicon Sequencing
por: Morgan, Horatio H., et al.
Publicado: (2017)

Simultaneous absolute quantification and sequencing of fish environmental DNA in a mesocosm by quantitative sequencing technique
por: Hoshino, Tatsuhiko, et al.
Publicado: (2021)

NanoAmpli-Seq: a workflow for amplicon sequencing for mixed microbial communities on the nanopore sequencing platform
por: Calus, Szymon T, et al.
Publicado: (2018)

Temperature-Induced Annual Variation in Microbial Community Changes and Resulting Metabolome Shifts in a Controlled Fermentation System
por: Wang, Shilei, et al.
Publicado: (2020)

Metagenomic Quantification of Genes with Internal Standards
por: Crossette, Emily, et al.
Publicado: (2021)

Invasion of Spartina alterniflora on Zostera japonica enhances the abundances of bacteria by absolute quantification sequencing analysis
por: Song, Zenglei, et al.
Publicado: (2022)

Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing
por: Tourlousse, Dieter M., et al.
Publicado: (2017)

SESAME (SEquence Sorter & AMplicon Explorer): genotyping based on high-throughput multiplex amplicon sequencing
por: Meglécz, Emese, et al.
Publicado: (2011)

Towards standardization of absolute SPECT/CT quantification: a multi-center and multi-vendor phantom study
por: Peters, Steffie M. B., et al.
Publicado: (2019)

Effects of Chemical and Mechanical Lysis on Microbial DNA Yield, Integrity, and Downstream Amplicon Sequencing of Rumen Bacteria and Protozoa
por: Ma, Zhi Yuan, et al.
Publicado: (2020)

Evaluation of Digital PCR for Absolute RNA Quantification
por: Sanders, Rebecca, et al.
Publicado: (2013)

Absolute Quantification of Endogenous Ras Isoform Abundance
por: Mageean, Craig J., et al.
Publicado: (2015)

Absolute Quantification of Amyloid Propagons by Digital Microfluidics
por: Pfammatter, Manuela, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_u04n0kivm4qcaor83tmam7gfqq