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Gradient Internal Standard Method for Absolute Quantification of Microbial Amplicon Sequencing Data

High-throughput amplicon sequencing is a critical tool for studying microbiota; however, it results only in relative abundance data. Thus, changes in absolute abundance of microbiota cannot be determined, which hinders further microbiology research. We have therefore established a gradient internal...

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Detalles Bibliográficos
Autores principales: Wang, Shilei, Wu, Qun, Han, Ying, Du, Rubing, Wang, Xiaoyong, Nie, Yao, Du, Xiaowei, Xu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901480/
https://www.ncbi.nlm.nih.gov/pubmed/33436513
http://dx.doi.org/10.1128/mSystems.00964-20

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