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Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L.

Scientists anxiously follow instances of heavy metals augmenting in the environment and undergoing bioaccumulation and trace their biomagnification across food webs, wary of their potent toxicity on biological entities. Engineered nanoparticles supplement natural pools of respective heavy metals and...

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Autores principales: Manna, Indrani, Sahoo, Saikat, Bandyopadhyay, Maumita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901573/
https://www.ncbi.nlm.nih.gov/pubmed/33633755
http://dx.doi.org/10.3389/fpls.2021.586509
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author Manna, Indrani
Sahoo, Saikat
Bandyopadhyay, Maumita
author_facet Manna, Indrani
Sahoo, Saikat
Bandyopadhyay, Maumita
author_sort Manna, Indrani
collection PubMed
description Scientists anxiously follow instances of heavy metals augmenting in the environment and undergoing bioaccumulation and trace their biomagnification across food webs, wary of their potent toxicity on biological entities. Engineered nanoparticles supplement natural pools of respective heavy metals and can mimic their effects, exerting toxicity at higher concentrations. Thus, a thorough understanding of the underlying mechanism of this precarious interaction is mandatory. Most urban and industrial environments contain considerable quantities of nickel oxide nanoparticles. These in excess can cause considerable damage to plant metabolism through a significant increase in cellular reactive oxygen species and perturbation of its cross-talk with the reactive nitrogen species. In the present work, the authors have demonstrated how the intrusion of nickel oxide nanoparticles (NiO-NP) affected the exposed roots of Allium cepa: starting with disruption of cell membranes, before being interiorized within cell organelles, effectively disrupting cellular homeostasis and survival. A major shift in the reactive oxygen species (ROS) and nitric oxide (NO) equanimity was also observed, unleashing major altercations in several crucial biochemical profiles. Altered antioxidant contents and upregulation of stress-responsive genes, namely, Catalase, Ascorbate peroxidase, Superoxide dismutase, and Rubisco activase, showing on average 50–250% rise across NiO-NP concentrations tested, also entailed increased cellular hydrogen peroxide contents, with tandem rise in cellular NO. Increased NO content was evinced from altered concentrations of nitric oxide synthase and nitrate reductase, along with NADPH oxidase, when compared with the negative control. Though initially showing a dose-dependent concomitant rise, a significant decrease of NO was observed at higher concentrations of NiO-NP, while cellular ROS continued to increase. Modified K/Na ratios, with increased proline concentrations and GABA contents, all hallmarks of cellular stress, correlated with ROS–NO perturbations. Detailed studies showed that NiO-NP concentration had a significant role in inducing toxicity, perturbing the fine balance of ROS–NO, which turned lethal for the cell at higher dosages of the ENP precipitating in the accumulation of stress markers and an inevitable shutdown of cellular mechanisms.
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spelling pubmed-79015732021-02-24 Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L. Manna, Indrani Sahoo, Saikat Bandyopadhyay, Maumita Front Plant Sci Plant Science Scientists anxiously follow instances of heavy metals augmenting in the environment and undergoing bioaccumulation and trace their biomagnification across food webs, wary of their potent toxicity on biological entities. Engineered nanoparticles supplement natural pools of respective heavy metals and can mimic their effects, exerting toxicity at higher concentrations. Thus, a thorough understanding of the underlying mechanism of this precarious interaction is mandatory. Most urban and industrial environments contain considerable quantities of nickel oxide nanoparticles. These in excess can cause considerable damage to plant metabolism through a significant increase in cellular reactive oxygen species and perturbation of its cross-talk with the reactive nitrogen species. In the present work, the authors have demonstrated how the intrusion of nickel oxide nanoparticles (NiO-NP) affected the exposed roots of Allium cepa: starting with disruption of cell membranes, before being interiorized within cell organelles, effectively disrupting cellular homeostasis and survival. A major shift in the reactive oxygen species (ROS) and nitric oxide (NO) equanimity was also observed, unleashing major altercations in several crucial biochemical profiles. Altered antioxidant contents and upregulation of stress-responsive genes, namely, Catalase, Ascorbate peroxidase, Superoxide dismutase, and Rubisco activase, showing on average 50–250% rise across NiO-NP concentrations tested, also entailed increased cellular hydrogen peroxide contents, with tandem rise in cellular NO. Increased NO content was evinced from altered concentrations of nitric oxide synthase and nitrate reductase, along with NADPH oxidase, when compared with the negative control. Though initially showing a dose-dependent concomitant rise, a significant decrease of NO was observed at higher concentrations of NiO-NP, while cellular ROS continued to increase. Modified K/Na ratios, with increased proline concentrations and GABA contents, all hallmarks of cellular stress, correlated with ROS–NO perturbations. Detailed studies showed that NiO-NP concentration had a significant role in inducing toxicity, perturbing the fine balance of ROS–NO, which turned lethal for the cell at higher dosages of the ENP precipitating in the accumulation of stress markers and an inevitable shutdown of cellular mechanisms. Frontiers Media S.A. 2021-02-09 /pmc/articles/PMC7901573/ /pubmed/33633755 http://dx.doi.org/10.3389/fpls.2021.586509 Text en Copyright © 2021 Manna, Sahoo and Bandyopadhyay. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Manna, Indrani
Sahoo, Saikat
Bandyopadhyay, Maumita
Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L.
title Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L.
title_full Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L.
title_fullStr Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L.
title_full_unstemmed Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L.
title_short Effect of Engineered Nickel Oxide Nanoparticle on Reactive Oxygen Species–Nitric Oxide Interplay in the Roots of Allium cepa L.
title_sort effect of engineered nickel oxide nanoparticle on reactive oxygen species–nitric oxide interplay in the roots of allium cepa l.
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901573/
https://www.ncbi.nlm.nih.gov/pubmed/33633755
http://dx.doi.org/10.3389/fpls.2021.586509
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