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Serum Level of Transferrin Unique Peptide Is Decreased in Patients With Acute Ischemic Stroke
Objectives: Free irons are transported into brain tissues by transferrin and play an important role in neuronal/glial cell damage. Lower serum levels of transferrin have been found in patients with ischemic stroke, compared with healthy subjects. In present study, we investigated whether transferrin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901928/ https://www.ncbi.nlm.nih.gov/pubmed/33633672 http://dx.doi.org/10.3389/fneur.2021.619310 |
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author | Hu, Xizheng Li, Yinghui Cheng, Peng Wu, Anhua Li, Guangyu |
author_facet | Hu, Xizheng Li, Yinghui Cheng, Peng Wu, Anhua Li, Guangyu |
author_sort | Hu, Xizheng |
collection | PubMed |
description | Objectives: Free irons are transported into brain tissues by transferrin and play an important role in neuronal/glial cell damage. Lower serum levels of transferrin have been found in patients with ischemic stroke, compared with healthy subjects. In present study, we investigated whether transferrin unique peptide (TF-UP) could be employed as a serum biomarker for brain tissue damage in acute ischemic stroke. Methods: The venous blood samples of 94 ischemic stroke patients and 35 brain tumor-stroke mimics (BT-SM) patients were collected within the first 72 h (Median time 23.25, Interquartile range 60.75) of acute onset in the emergency room. Total TF-UP and total albumin unique peptide (Alb-UP) were identified with liquid chromatography/mass spectrometry (LC–MS/MS) and quantified by multiple reaction monitoring (MRM) method using labeled reference peptide (LRP) for further analysis. Results: Median ratio of total TF-UP/LRP was 0.85 (Interquartile range, 0.21) in the brain tumor-stroke mimics (BT-SM) group, and 0.45 (0.14) in the ischemic stroke group; median Alb-UP/LRP ratio was 0.66 (0.16) in the BT-SM group, and 0.55 (0.20) in the ischemic stroke group. The overall trend from low to high levels was statistically significant for TF-UP/LRP (P < 0.0001), but not for Alb-UP/LRP (P = 0.1667). According to the receiver operating characteristic (ROC) curve, the area under the curve (AUC) was 0.9565 and the optimal cutoff value of serum TF-UP was 0.6317, which yielded a sensitivity of 91.49% and a specificity of 88.57%. The odds ratio (95% confidence intervals) of serum TF-UP/LRP was 83.31 (23.43, 296.22, P < 0.0001). Conclusions: Serum TF-UP/LRP level is decreased in patients with acute ischemic stroke in comparison with brain tumor, and it may serve as a serum biomarker for the neuronal/glial cell damage in cerebral infarction. |
format | Online Article Text |
id | pubmed-7901928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79019282021-02-24 Serum Level of Transferrin Unique Peptide Is Decreased in Patients With Acute Ischemic Stroke Hu, Xizheng Li, Yinghui Cheng, Peng Wu, Anhua Li, Guangyu Front Neurol Neurology Objectives: Free irons are transported into brain tissues by transferrin and play an important role in neuronal/glial cell damage. Lower serum levels of transferrin have been found in patients with ischemic stroke, compared with healthy subjects. In present study, we investigated whether transferrin unique peptide (TF-UP) could be employed as a serum biomarker for brain tissue damage in acute ischemic stroke. Methods: The venous blood samples of 94 ischemic stroke patients and 35 brain tumor-stroke mimics (BT-SM) patients were collected within the first 72 h (Median time 23.25, Interquartile range 60.75) of acute onset in the emergency room. Total TF-UP and total albumin unique peptide (Alb-UP) were identified with liquid chromatography/mass spectrometry (LC–MS/MS) and quantified by multiple reaction monitoring (MRM) method using labeled reference peptide (LRP) for further analysis. Results: Median ratio of total TF-UP/LRP was 0.85 (Interquartile range, 0.21) in the brain tumor-stroke mimics (BT-SM) group, and 0.45 (0.14) in the ischemic stroke group; median Alb-UP/LRP ratio was 0.66 (0.16) in the BT-SM group, and 0.55 (0.20) in the ischemic stroke group. The overall trend from low to high levels was statistically significant for TF-UP/LRP (P < 0.0001), but not for Alb-UP/LRP (P = 0.1667). According to the receiver operating characteristic (ROC) curve, the area under the curve (AUC) was 0.9565 and the optimal cutoff value of serum TF-UP was 0.6317, which yielded a sensitivity of 91.49% and a specificity of 88.57%. The odds ratio (95% confidence intervals) of serum TF-UP/LRP was 83.31 (23.43, 296.22, P < 0.0001). Conclusions: Serum TF-UP/LRP level is decreased in patients with acute ischemic stroke in comparison with brain tumor, and it may serve as a serum biomarker for the neuronal/glial cell damage in cerebral infarction. Frontiers Media S.A. 2021-02-05 /pmc/articles/PMC7901928/ /pubmed/33633672 http://dx.doi.org/10.3389/fneur.2021.619310 Text en Copyright © 2021 Hu, Li, Cheng, Wu and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neurology Hu, Xizheng Li, Yinghui Cheng, Peng Wu, Anhua Li, Guangyu Serum Level of Transferrin Unique Peptide Is Decreased in Patients With Acute Ischemic Stroke |
title | Serum Level of Transferrin Unique Peptide Is Decreased in Patients With Acute Ischemic Stroke |
title_full | Serum Level of Transferrin Unique Peptide Is Decreased in Patients With Acute Ischemic Stroke |
title_fullStr | Serum Level of Transferrin Unique Peptide Is Decreased in Patients With Acute Ischemic Stroke |
title_full_unstemmed | Serum Level of Transferrin Unique Peptide Is Decreased in Patients With Acute Ischemic Stroke |
title_short | Serum Level of Transferrin Unique Peptide Is Decreased in Patients With Acute Ischemic Stroke |
title_sort | serum level of transferrin unique peptide is decreased in patients with acute ischemic stroke |
topic | Neurology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901928/ https://www.ncbi.nlm.nih.gov/pubmed/33633672 http://dx.doi.org/10.3389/fneur.2021.619310 |
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