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Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 Patients

A subset of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) developed a condition of hyper-inflammation, which can cause multi-organ damage and the more severe forms of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) can promote tissue regenerat...

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Autores principales: Croci, Stefania, Bonacini, Martina, Dolci, Giovanni, Massari, Marco, Facciolongo, Nicola, Pignatti, Elisa, Pisciotta, Alessandra, Carnevale, Gianluca, Negro, Aurelio, Cassone, Giulia, Muratore, Francesco, Belloni, Lucia, Zerbini, Alessandro, Salvarani, Carlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901970/
https://www.ncbi.nlm.nih.gov/pubmed/33634100
http://dx.doi.org/10.3389/fcell.2020.609204
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author Croci, Stefania
Bonacini, Martina
Dolci, Giovanni
Massari, Marco
Facciolongo, Nicola
Pignatti, Elisa
Pisciotta, Alessandra
Carnevale, Gianluca
Negro, Aurelio
Cassone, Giulia
Muratore, Francesco
Belloni, Lucia
Zerbini, Alessandro
Salvarani, Carlo
author_facet Croci, Stefania
Bonacini, Martina
Dolci, Giovanni
Massari, Marco
Facciolongo, Nicola
Pignatti, Elisa
Pisciotta, Alessandra
Carnevale, Gianluca
Negro, Aurelio
Cassone, Giulia
Muratore, Francesco
Belloni, Lucia
Zerbini, Alessandro
Salvarani, Carlo
author_sort Croci, Stefania
collection PubMed
description A subset of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) developed a condition of hyper-inflammation, which can cause multi-organ damage and the more severe forms of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) can promote tissue regeneration and modulate immune responses and, thus, have the rational requirements to be used to counteract SARS-CoV-2-induced pneumonia and hyper-inflammation. The aim of the present study was to gain insight into possible mechanisms of action of MSCs obtained from human dental pulp [dental pulp stem cells (DPSCs)] in COVID-19 patients. We investigated the concentrations of 18 cytokines in supernatants of peripheral blood mononuclear cells (PBMCs) obtained from COVID-19 patients cultured in vitro alone and in contact with DPSCs. The modulation of cytokines in PBMCs was confirmed by real-time PCR. IL-6 was the sole cytokine detected in supernatants of DPSCs. In resting conditions, co-culture increased IL-1β, IL-2, IL-5, IL-6, IL-10, IL-18, TNFα, and granulocyte macrophage colony-stimulating factor (GM-CSF) levels. When PBMCs were activated with anti-CD3/CD28 antibody-coated beads, co-culture increased IL-6 and GM-CSF, whereas it decreased IFNγ, TNFα, IL-2, IL-5, IL-9, IL-10, IL-12 (p70), IL-17A, IL-18, IL-21, IL-23, and IL-27 levels. Concentrations of IL-1β, IL-4, IL-13, and IL-22 were not affected. The comparison of cytokine concentrations in supernatants of PBMCs from COVID-19 patients vs. healthy subjects revealed lower concentrations of IL-10 and higher concentrations of IL-18 in supernatants of CD3/CD28-activated PBMCs from COVID-19 patients. Results are explorative but indicate that DPSCs can modulate the production of cytokines deregulated in COVID-19 patients, supporting their potential use in COVID-19.
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spelling pubmed-79019702021-02-24 Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 Patients Croci, Stefania Bonacini, Martina Dolci, Giovanni Massari, Marco Facciolongo, Nicola Pignatti, Elisa Pisciotta, Alessandra Carnevale, Gianluca Negro, Aurelio Cassone, Giulia Muratore, Francesco Belloni, Lucia Zerbini, Alessandro Salvarani, Carlo Front Cell Dev Biol Cell and Developmental Biology A subset of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) developed a condition of hyper-inflammation, which can cause multi-organ damage and the more severe forms of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) can promote tissue regeneration and modulate immune responses and, thus, have the rational requirements to be used to counteract SARS-CoV-2-induced pneumonia and hyper-inflammation. The aim of the present study was to gain insight into possible mechanisms of action of MSCs obtained from human dental pulp [dental pulp stem cells (DPSCs)] in COVID-19 patients. We investigated the concentrations of 18 cytokines in supernatants of peripheral blood mononuclear cells (PBMCs) obtained from COVID-19 patients cultured in vitro alone and in contact with DPSCs. The modulation of cytokines in PBMCs was confirmed by real-time PCR. IL-6 was the sole cytokine detected in supernatants of DPSCs. In resting conditions, co-culture increased IL-1β, IL-2, IL-5, IL-6, IL-10, IL-18, TNFα, and granulocyte macrophage colony-stimulating factor (GM-CSF) levels. When PBMCs were activated with anti-CD3/CD28 antibody-coated beads, co-culture increased IL-6 and GM-CSF, whereas it decreased IFNγ, TNFα, IL-2, IL-5, IL-9, IL-10, IL-12 (p70), IL-17A, IL-18, IL-21, IL-23, and IL-27 levels. Concentrations of IL-1β, IL-4, IL-13, and IL-22 were not affected. The comparison of cytokine concentrations in supernatants of PBMCs from COVID-19 patients vs. healthy subjects revealed lower concentrations of IL-10 and higher concentrations of IL-18 in supernatants of CD3/CD28-activated PBMCs from COVID-19 patients. Results are explorative but indicate that DPSCs can modulate the production of cytokines deregulated in COVID-19 patients, supporting their potential use in COVID-19. Frontiers Media S.A. 2021-02-05 /pmc/articles/PMC7901970/ /pubmed/33634100 http://dx.doi.org/10.3389/fcell.2020.609204 Text en Copyright © 2021 Croci, Bonacini, Dolci, Massari, Facciolongo, Pignatti, Pisciotta, Carnevale, Negro, Cassone, Muratore, Belloni, Zerbini and Salvarani. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Croci, Stefania
Bonacini, Martina
Dolci, Giovanni
Massari, Marco
Facciolongo, Nicola
Pignatti, Elisa
Pisciotta, Alessandra
Carnevale, Gianluca
Negro, Aurelio
Cassone, Giulia
Muratore, Francesco
Belloni, Lucia
Zerbini, Alessandro
Salvarani, Carlo
Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 Patients
title Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 Patients
title_full Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 Patients
title_fullStr Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 Patients
title_full_unstemmed Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 Patients
title_short Human Dental Pulp Stem Cells Modulate Cytokine Production in vitro by Peripheral Blood Mononuclear Cells From Coronavirus Disease 2019 Patients
title_sort human dental pulp stem cells modulate cytokine production in vitro by peripheral blood mononuclear cells from coronavirus disease 2019 patients
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901970/
https://www.ncbi.nlm.nih.gov/pubmed/33634100
http://dx.doi.org/10.3389/fcell.2020.609204
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