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High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis

In this study, we reported the prevalence and mechanism associated with the extended-spectrum beta-lactamase (ESBL)-positive phenotype in Laribacter hongkongensis isolated from patients and fish. Using the inhibition zone enhancement test, 20 (95.2%) of the 21 patient strains and 8 (57.1%) of the 14...

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Autores principales: Teng, Jade L. L., Luo, Ruibang, Tang, Bone S. F., Fong, Jordan Y. H., Wang, Li, Jia, Lilong, Wong, Chloe K. S., Chan, Elaine, Leung, Amy W. S., Siu, Gilman K. H., Chiu, Tsz-Ho, Fung, Ami M. Y., Wu, Alan K. L., Yeung, Man-Lung, Lau, Susanna K. P., Woo, Patrick C. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902055/
https://www.ncbi.nlm.nih.gov/pubmed/33633706
http://dx.doi.org/10.3389/fmicb.2021.618894
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author Teng, Jade L. L.
Luo, Ruibang
Tang, Bone S. F.
Fong, Jordan Y. H.
Wang, Li
Jia, Lilong
Wong, Chloe K. S.
Chan, Elaine
Leung, Amy W. S.
Siu, Gilman K. H.
Chiu, Tsz-Ho
Fung, Ami M. Y.
Wu, Alan K. L.
Yeung, Man-Lung
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_facet Teng, Jade L. L.
Luo, Ruibang
Tang, Bone S. F.
Fong, Jordan Y. H.
Wang, Li
Jia, Lilong
Wong, Chloe K. S.
Chan, Elaine
Leung, Amy W. S.
Siu, Gilman K. H.
Chiu, Tsz-Ho
Fung, Ami M. Y.
Wu, Alan K. L.
Yeung, Man-Lung
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_sort Teng, Jade L. L.
collection PubMed
description In this study, we reported the prevalence and mechanism associated with the extended-spectrum beta-lactamase (ESBL)-positive phenotype in Laribacter hongkongensis isolated from patients and fish. Using the inhibition zone enhancement test, 20 (95.2%) of the 21 patient strains and 8 (57.1%) of the 14 fish strains were tested ESBL-positive. However, ESBL genes, including SHV, TEM, CTX-M, GES, and PER, were not detected in all of these 28 L. hongkongensis isolates. No ESBL gene could be detected in either the complete genome of L. hongkongensis HLHK9 or the draft genome of PW3643. PCR and DNA sequencing revealed that all the 35 L. hongkongensis isolates (showing both ESBL-positive and ESBL-negative phenotypes) were positive for the ampC gene. When the AmpC deletion mutant, HLHK9ΔampC, was subject to the zone enhancement test, the difference of zone size between ceftazidime/clavulanate and ceftazidime was less than 5 mm. When boronic acid was added to the antibiotic disks, none of the 28 “ESBL-positive” isolates showed a ≥ 5 mm enhancement of inhibition zone size diameter between ceftazidime/clavulanate and ceftazidime and between cefotaxime/clavulanate and cefotaxime. A high prevalence (80%) of ESBL-positive phenotype is present in L. hongkongensis. Overall, our results suggested that the ESBL-positive phenotype in L. hongkongensis results from the expression of the intrinsic AmpC beta-lactamase. Confirmatory tests should be performed before issuing laboratory reports for L. hongkongensis isolates that are tested ESBL-positive by disk diffusion clavulanate inhibition test.
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spelling pubmed-79020552021-02-24 High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis Teng, Jade L. L. Luo, Ruibang Tang, Bone S. F. Fong, Jordan Y. H. Wang, Li Jia, Lilong Wong, Chloe K. S. Chan, Elaine Leung, Amy W. S. Siu, Gilman K. H. Chiu, Tsz-Ho Fung, Ami M. Y. Wu, Alan K. L. Yeung, Man-Lung Lau, Susanna K. P. Woo, Patrick C. Y. Front Microbiol Microbiology In this study, we reported the prevalence and mechanism associated with the extended-spectrum beta-lactamase (ESBL)-positive phenotype in Laribacter hongkongensis isolated from patients and fish. Using the inhibition zone enhancement test, 20 (95.2%) of the 21 patient strains and 8 (57.1%) of the 14 fish strains were tested ESBL-positive. However, ESBL genes, including SHV, TEM, CTX-M, GES, and PER, were not detected in all of these 28 L. hongkongensis isolates. No ESBL gene could be detected in either the complete genome of L. hongkongensis HLHK9 or the draft genome of PW3643. PCR and DNA sequencing revealed that all the 35 L. hongkongensis isolates (showing both ESBL-positive and ESBL-negative phenotypes) were positive for the ampC gene. When the AmpC deletion mutant, HLHK9ΔampC, was subject to the zone enhancement test, the difference of zone size between ceftazidime/clavulanate and ceftazidime was less than 5 mm. When boronic acid was added to the antibiotic disks, none of the 28 “ESBL-positive” isolates showed a ≥ 5 mm enhancement of inhibition zone size diameter between ceftazidime/clavulanate and ceftazidime and between cefotaxime/clavulanate and cefotaxime. A high prevalence (80%) of ESBL-positive phenotype is present in L. hongkongensis. Overall, our results suggested that the ESBL-positive phenotype in L. hongkongensis results from the expression of the intrinsic AmpC beta-lactamase. Confirmatory tests should be performed before issuing laboratory reports for L. hongkongensis isolates that are tested ESBL-positive by disk diffusion clavulanate inhibition test. Frontiers Media S.A. 2021-02-09 /pmc/articles/PMC7902055/ /pubmed/33633706 http://dx.doi.org/10.3389/fmicb.2021.618894 Text en Copyright © 2021 Teng, Luo, Tang, Fong, Wang, Jia, Wong, Chan, Leung, Siu, Chiu, Fung, Wu, Yeung, Lau and Woo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Teng, Jade L. L.
Luo, Ruibang
Tang, Bone S. F.
Fong, Jordan Y. H.
Wang, Li
Jia, Lilong
Wong, Chloe K. S.
Chan, Elaine
Leung, Amy W. S.
Siu, Gilman K. H.
Chiu, Tsz-Ho
Fung, Ami M. Y.
Wu, Alan K. L.
Yeung, Man-Lung
Lau, Susanna K. P.
Woo, Patrick C. Y.
High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis
title High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis
title_full High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis
title_fullStr High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis
title_full_unstemmed High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis
title_short High Prevalence and Mechanism Associated With Extended Spectrum Beta-Lactamase-Positive Phenotype in Laribacter hongkongensis
title_sort high prevalence and mechanism associated with extended spectrum beta-lactamase-positive phenotype in laribacter hongkongensis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902055/
https://www.ncbi.nlm.nih.gov/pubmed/33633706
http://dx.doi.org/10.3389/fmicb.2021.618894
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