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Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system

It has been reported in recent studies that restraint stress on pregnant mice during the preimplantation stage elevated corticotrophin-releasing hormone (CRH) and glucocorticoid levels in the serum and oviducts; furthermore, CRH and corticosterone (CORT) impacted preimplantation embryos indirectly b...

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Autores principales: ZHAO, Ying-Qi, CHEN, Ren-Ren, KONG, Qiao-Qiao, AN, Jin-Song, ZHAO, Xin-Yue, GONG, Shuai, YUAN, Hong-Jie, TAN, Jing-He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902214/
https://www.ncbi.nlm.nih.gov/pubmed/33310974
http://dx.doi.org/10.1262/jrd.2020-122
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author ZHAO, Ying-Qi
CHEN, Ren-Ren
KONG, Qiao-Qiao
AN, Jin-Song
ZHAO, Xin-Yue
GONG, Shuai
YUAN, Hong-Jie
TAN, Jing-He
author_facet ZHAO, Ying-Qi
CHEN, Ren-Ren
KONG, Qiao-Qiao
AN, Jin-Song
ZHAO, Xin-Yue
GONG, Shuai
YUAN, Hong-Jie
TAN, Jing-He
author_sort ZHAO, Ying-Qi
collection PubMed
description It has been reported in recent studies that restraint stress on pregnant mice during the preimplantation stage elevated corticotrophin-releasing hormone (CRH) and glucocorticoid levels in the serum and oviducts; furthermore, CRH and corticosterone (CORT) impacted preimplantation embryos indirectly by triggering the apoptosis of oviductal epithelial cells (OECs) through activation of the Fas system. However, it remains unclear whether TNF-α signaling is involved in CRH- and/or glucocorticoid-induced apoptosis of OECs. In the present study, it was shown that culture with either CRH or CORT induced significant apoptosis of OECs. The culture of OECs with CRH augmented both FasL expression and TNF-α expression. However, culture with CORT increased FasL, but decreased TNF-α, expression significantly. Although knocking down/knocking out FasL expression in OECs significantly ameliorated the proapoptotic effects of both CRH and CORT, knocking down/knocking out TNF-α expression relieved only the proapoptotic effect of CRH but not that of CORT. Taken together, our results demonstrated that CRH-induced OEC apoptosis involved both Fas signaling and TNF-α signaling. Conversely, CORT-induced OEC apoptosis involved only the Fas, but not the TNF-α, signaling pathway. The data obtained are crucial for our understanding of the mechanisms by which various categories of stress imposed on pregnant females impair embryo development, as well as for the development of measures to protect the embryo from the adverse effects of stress.
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spelling pubmed-79022142021-03-01 Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system ZHAO, Ying-Qi CHEN, Ren-Ren KONG, Qiao-Qiao AN, Jin-Song ZHAO, Xin-Yue GONG, Shuai YUAN, Hong-Jie TAN, Jing-He J Reprod Dev Original Article It has been reported in recent studies that restraint stress on pregnant mice during the preimplantation stage elevated corticotrophin-releasing hormone (CRH) and glucocorticoid levels in the serum and oviducts; furthermore, CRH and corticosterone (CORT) impacted preimplantation embryos indirectly by triggering the apoptosis of oviductal epithelial cells (OECs) through activation of the Fas system. However, it remains unclear whether TNF-α signaling is involved in CRH- and/or glucocorticoid-induced apoptosis of OECs. In the present study, it was shown that culture with either CRH or CORT induced significant apoptosis of OECs. The culture of OECs with CRH augmented both FasL expression and TNF-α expression. However, culture with CORT increased FasL, but decreased TNF-α, expression significantly. Although knocking down/knocking out FasL expression in OECs significantly ameliorated the proapoptotic effects of both CRH and CORT, knocking down/knocking out TNF-α expression relieved only the proapoptotic effect of CRH but not that of CORT. Taken together, our results demonstrated that CRH-induced OEC apoptosis involved both Fas signaling and TNF-α signaling. Conversely, CORT-induced OEC apoptosis involved only the Fas, but not the TNF-α, signaling pathway. The data obtained are crucial for our understanding of the mechanisms by which various categories of stress imposed on pregnant females impair embryo development, as well as for the development of measures to protect the embryo from the adverse effects of stress. The Society for Reproduction and Development 2020-12-12 2021-02 /pmc/articles/PMC7902214/ /pubmed/33310974 http://dx.doi.org/10.1262/jrd.2020-122 Text en ©2021 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
ZHAO, Ying-Qi
CHEN, Ren-Ren
KONG, Qiao-Qiao
AN, Jin-Song
ZHAO, Xin-Yue
GONG, Shuai
YUAN, Hong-Jie
TAN, Jing-He
Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system
title Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system
title_full Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system
title_fullStr Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system
title_full_unstemmed Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system
title_short Corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the TNF-α system
title_sort corticosterone induced apoptosis of mouse oviduct epithelial cells independent of the tnf-α system
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902214/
https://www.ncbi.nlm.nih.gov/pubmed/33310974
http://dx.doi.org/10.1262/jrd.2020-122
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