Cargando…
Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study
BACKGROUND: Community‐based studies of influenza and other respiratory viruses (eg, SARS‐CoV‐2) require laboratory confirmation of infection. During the current COVID‐19 pandemic, social distancing guidelines require alternative data collection in order to protect both research staff and participant...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902250/ https://www.ncbi.nlm.nih.gov/pubmed/33107200 http://dx.doi.org/10.1111/irv.12822 |
_version_ | 1783654521630621696 |
---|---|
author | Malosh, Ryan E. Petrie, Joshua G. Callear, Amy P. Monto, Arnold S. Martin, Emily T. |
author_facet | Malosh, Ryan E. Petrie, Joshua G. Callear, Amy P. Monto, Arnold S. Martin, Emily T. |
author_sort | Malosh, Ryan E. |
collection | PubMed |
description | BACKGROUND: Community‐based studies of influenza and other respiratory viruses (eg, SARS‐CoV‐2) require laboratory confirmation of infection. During the current COVID‐19 pandemic, social distancing guidelines require alternative data collection in order to protect both research staff and participants. Home‐collected respiratory specimens are less resource‐intensive, can be collected earlier after symptom onset, and provide a low‐contact means of data collection. A prospective, multi‐year, community‐based cohort study is an ideal setting to examine the utility of home‐collected specimens for identification of influenza. METHODS: We describe the feasibility and reliability of home‐collected specimens for the detection of influenza. We collected data and specimens between October 2014 and June 2017 from the Household Influenza Vaccine Evaluation (HIVE) Study. Cohort participants were asked to collect a nasal swab at home upon onset of acute respiratory illness. Research staff also collected nose and throat swab specimens in the study clinic within 7 days of onset. We estimated agreement using Cohen's kappa and calculated sensitivity and specificity of home‐collected compared to staff‐collected specimens. RESULTS: We tested 336 paired staff‐ and home‐collected respiratory specimens for influenza by RT‐PCR; 150 staff‐collected specimens were positive for influenza A/H3N2, 23 for influenza A/H1N1, 14 for influenza B/Victoria, and 31 for influenza B/Yamagata. We found moderate agreement between collection methods for influenza A/H3N2 (0.70) and B/Yamagata (0.69) and high agreement for influenza A/H1N1 (0.87) and B/Victoria (0.86). Sensitivity ranged from 78% to 86% for all influenza types and subtypes. Specificity was high for influenza A/H1N1 and both influenza B lineages with a range from 96% to 100%, and slightly lower for A/H3N2 infections (88%). CONCLUSIONS: Collection of nasal swab specimens at home is both feasible and reliable for identification of influenza virus infections. |
format | Online Article Text |
id | pubmed-7902250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79022502021-03-03 Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study Malosh, Ryan E. Petrie, Joshua G. Callear, Amy P. Monto, Arnold S. Martin, Emily T. Influenza Other Respir Viruses Original Articles BACKGROUND: Community‐based studies of influenza and other respiratory viruses (eg, SARS‐CoV‐2) require laboratory confirmation of infection. During the current COVID‐19 pandemic, social distancing guidelines require alternative data collection in order to protect both research staff and participants. Home‐collected respiratory specimens are less resource‐intensive, can be collected earlier after symptom onset, and provide a low‐contact means of data collection. A prospective, multi‐year, community‐based cohort study is an ideal setting to examine the utility of home‐collected specimens for identification of influenza. METHODS: We describe the feasibility and reliability of home‐collected specimens for the detection of influenza. We collected data and specimens between October 2014 and June 2017 from the Household Influenza Vaccine Evaluation (HIVE) Study. Cohort participants were asked to collect a nasal swab at home upon onset of acute respiratory illness. Research staff also collected nose and throat swab specimens in the study clinic within 7 days of onset. We estimated agreement using Cohen's kappa and calculated sensitivity and specificity of home‐collected compared to staff‐collected specimens. RESULTS: We tested 336 paired staff‐ and home‐collected respiratory specimens for influenza by RT‐PCR; 150 staff‐collected specimens were positive for influenza A/H3N2, 23 for influenza A/H1N1, 14 for influenza B/Victoria, and 31 for influenza B/Yamagata. We found moderate agreement between collection methods for influenza A/H3N2 (0.70) and B/Yamagata (0.69) and high agreement for influenza A/H1N1 (0.87) and B/Victoria (0.86). Sensitivity ranged from 78% to 86% for all influenza types and subtypes. Specificity was high for influenza A/H1N1 and both influenza B lineages with a range from 96% to 100%, and slightly lower for A/H3N2 infections (88%). CONCLUSIONS: Collection of nasal swab specimens at home is both feasible and reliable for identification of influenza virus infections. John Wiley and Sons Inc. 2020-10-26 2021-03 /pmc/articles/PMC7902250/ /pubmed/33107200 http://dx.doi.org/10.1111/irv.12822 Text en © 2020 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Malosh, Ryan E. Petrie, Joshua G. Callear, Amy P. Monto, Arnold S. Martin, Emily T. Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study |
title | Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study |
title_full | Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study |
title_fullStr | Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study |
title_full_unstemmed | Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study |
title_short | Home collection of nasal swabs for detection of influenza in the Household Influenza Vaccine Evaluation Study |
title_sort | home collection of nasal swabs for detection of influenza in the household influenza vaccine evaluation study |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902250/ https://www.ncbi.nlm.nih.gov/pubmed/33107200 http://dx.doi.org/10.1111/irv.12822 |
work_keys_str_mv | AT maloshryane homecollectionofnasalswabsfordetectionofinfluenzainthehouseholdinfluenzavaccineevaluationstudy AT petriejoshuag homecollectionofnasalswabsfordetectionofinfluenzainthehouseholdinfluenzavaccineevaluationstudy AT callearamyp homecollectionofnasalswabsfordetectionofinfluenzainthehouseholdinfluenzavaccineevaluationstudy AT montoarnolds homecollectionofnasalswabsfordetectionofinfluenzainthehouseholdinfluenzavaccineevaluationstudy AT martinemilyt homecollectionofnasalswabsfordetectionofinfluenzainthehouseholdinfluenzavaccineevaluationstudy |