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Suspension culture in a T-flask with acoustic flow induced by ultrasonic irradiation
Suspension culture is an essential large-scale cell culture technique for biopharmaceutical development and regenerative medicine. To transition from monolayer culture on the culture surface of a flask to suspension culture in a bioreactor, a pre-specified cell number must first be reached. During t...
Autores principales: | Fujii, Genichiro, Kurashina, Yuta, Terao, Yusuke, Azuma, Tetsushi, Morikawa, Akira, Kodeki, Kazuhide, Takahara, Osamu, Takemura, Kenjiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902488/ https://www.ncbi.nlm.nih.gov/pubmed/33607592 http://dx.doi.org/10.1016/j.ultsonch.2021.105488 |
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