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Purification and cryoelectron microscopy structure determination of human V-ATPase

Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are multi-component, ATP-driven proton pumps, which play important roles in many physiological processes by acidifying intracellular vesicles, organelles, and the extracellular milieu. Long-standing challenges in purifying mammalian V...

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Detalles Bibliográficos
Autores principales:	Wang, Longfei, Chen, Zhenhang, Wu, Hao, Fu, Tian-Min
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902551/ https://www.ncbi.nlm.nih.gov/pubmed/33665630 http://dx.doi.org/10.1016/j.xpro.2021.100350

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