Cargando…

A streamlined clinical metagenomic sequencing protocol for rapid pathogen identification

Metagenomic next-generation sequencing (mNGS) holds promise as a diagnostic tool for unbiased pathogen identification and precision medicine. However, its medical utility depends largely on assay simplicity and reproducibility. In the current study, we aimed to develop a streamlined Illumina and Oxf...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jia, Xiaofang, Hu, Lvyin, Wu, Min, Ling, Yun, Wang, Wei, Lu, Hongzhou, Yuan, Zhenghong, Yi, Zhigang, Zhang, Xiaonan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902651/ https://www.ncbi.nlm.nih.gov/pubmed/33623127 http://dx.doi.org/10.1038/s41598-021-83812-x

Ejemplares similares

Metaphor—A workflow for streamlined assembly and binning of metagenomes
por: Salazar, Vinícius W, et al.
Publicado: (2023)

Rapid screening and identification of viral pathogens in metagenomic data
por: Song, Shiyang, et al.
Publicado: (2021)

Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis
por: Greninger, Alexander L., et al.
Publicado: (2015)

MEDI: Macronutrient Extraction and Determination from invertebrates, a rapid, cheap and streamlined protocol
por: Cuff, Jordan P., et al.
Publicado: (2021)

Rapid Detection and Identification of Infectious Pathogens Based on High-throughput Sequencing
por: Ni, Pei-Xiang, et al.
Publicado: (2015)

PAIPline: pathogen identification in metagenomic and clinical next generation sequencing samples
por: Andrusch, Andreas, et al.
Publicado: (2018)

Metagenomic sequencing for detection and identification of the boxwood blight pathogen Calonectria pseudonaviculata
por: Yang, Shu, et al.
Publicado: (2022)

Functional mapping of B-cell linear epitopes of SARS-CoV-2 in COVID-19 convalescent population
por: Yi, Zhigang, et al.
Publicado: (2020)

Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood
por: Frey, Kenneth G, et al.
Publicado: (2014)

Identification of Pathogens Directly From Diabetic Foot Infections by Shotgun Metagenomic Sequencing
por: Shurko, James, et al.
Publicado: (2017)

Development of novel parameters for pathogen identification in clinical metagenomic next-generation sequencing
por: Jiang, Xiwen, et al.
Publicado: (2023)

Metagenomic next-generation sequencing for identification of central nervous system pathogens in HIV-infected patients
por: Zhu, Yunqi, et al.
Publicado: (2022)

Rapid metagenomics analysis of EMS vehicles for monitoring pathogen load using nanopore DNA sequencing
por: Sheahan, Taylor, et al.
Publicado: (2019)

Identification of α-Synuclein Proaggregator: Rapid Synthesis and Streamlining RT-QuIC Assays in Parkinson’s Disease
por: Takada, Fumito, et al.
Publicado: (2022)

A streamlined tethered chromosome conformation capture protocol
por: Gabdank, Idan, et al.
Publicado: (2016)

SMAP: a streamlined methylation analysis pipeline for bisulfite sequencing
por: Gao, Shengjie, et al.
Publicado: (2015)

Co-infection in COVID-19, a cohort study
por: Song, Wuhui, et al.
Publicado: (2021)

Direct detection and identification of periprosthetic joint infection pathogens by metagenomic next-generation sequencing
por: Hao, Linjie, et al.
Publicado: (2023)

Metagenomic sequencing of post-mortem tissue samples for the identification of pathogens associated with neonatal deaths
por: Baillie, Vicky L., et al.
Publicado: (2023)

American streamline
por: Hartley, Bernard
Publicado: (1983)

Streamlining in 2008
por: Einspahr, Howard, et al.
Publicado: (2008)

Centrifuge: rapid and sensitive classification of metagenomic sequences
por: Kim, Daehwan, et al.
Publicado: (2016)

Streamlined analysis of duplex sequencing data with Du Novo
por: Stoler, Nicholas, et al.
Publicado: (2016)

Streamlining Quantitative Analysis of Long RNA Sequencing Reads
por: Oeck, Sebastian, et al.
Publicado: (2020)

Metagenomic analysis and identification of emerging pathogens in blood from healthy donors
por: Xu, Min, et al.
Publicado: (2020)

Utilizing Metagenomic Next-Generation Sequencing (mNGS) for Rapid Pathogen Identification and to Inform Clinical Decision-Making: Results from a Large Real-World Cohort
por: Xu, Jie, et al.
Publicado: (2023)

Streamlined phage display library protocols for identification of insect gut binding peptides highlight peptide specificity
por: Mishra, Ruchir, et al.
Publicado: (2021)

Streamlining staging of lung and colorectal cancer with whole body MRI; study protocols for two multicentre, non-randomised, single-arm, prospective diagnostic accuracy studies (Streamline C and Streamline L)
por: Taylor, Stuart A., et al.
Publicado: (2017)

Direct Detection and Identification of Prosthetic Joint Pathogens in Synovial Fluid (SF) by Metagenomic Shotgun Sequencing
por: Ivy, Morgan, et al.
Publicado: (2017)

Case Report: Mycobacterium kansasii causing infective endocarditis explored by metagenomic next-generation sequencing
por: Yang, Liuqing, et al.
Publicado: (2023)

HabiSign: a novel approach for comparison of metagenomes and rapid identification of habitat-specific sequences
por: Ghosh, Tarini Shankar, et al.
Publicado: (2011)

Metagenomic Assay for Identification of Microbial Pathogens in Tumor Tissues
por: Baldwin, Don A., et al.
Publicado: (2014)

A streamlined search technology for identification of synergistic drug combinations
por: Weiss, Andrea, et al.
Publicado: (2015)

Metagenomic Sequencing Revealed the Potential Pathogenic Threats of Banknotes
por: Lin, Jun, et al.
Publicado: (2021)

Streamlined Genome Sequence Compression using Distributed Source Coding
por: Wang, Shuang, et al.
Publicado: (2014)

Nanopore-based metagenomic sequencing for the rapid and precise detection of pathogens among immunocompromised cancer patients with suspected infections
por: Deng, Qingmei, et al.
Publicado: (2022)

ve-SEQ: Robust, unbiased enrichment for streamlined detection and whole-genome sequencing of HCV and other highly diverse pathogens
por: Bonsall, David, et al.
Publicado: (2015)

Analysis Streamlining in ATLAS
por: Heinrich, Lukas, et al.
Publicado: (2018)

Streamlining publishing procedures
por: Van Herwijnen, Eric, et al.
Publicado: (1989)

Streamlined Process Improvement
por: Harrington, H
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_qcquiketkf926hmh2cl8qt9684