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Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing

The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large p...

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Autores principales: Aisen, Eduardo G., Huanca López, Wilfredo, Pérez Durand, Manuel G., Torres Mamani, Edita, Villanueva Mori, Juan C., Ousset, María J., Medina, Víctor H., Pérez Guerra, Uri H., Huanca Mamani, Teodosio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902725/
https://www.ncbi.nlm.nih.gov/pubmed/33644145
http://dx.doi.org/10.3389/fvets.2021.611301
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author Aisen, Eduardo G.
Huanca López, Wilfredo
Pérez Durand, Manuel G.
Torres Mamani, Edita
Villanueva Mori, Juan C.
Ousset, María J.
Medina, Víctor H.
Pérez Guerra, Uri H.
Huanca Mamani, Teodosio
author_facet Aisen, Eduardo G.
Huanca López, Wilfredo
Pérez Durand, Manuel G.
Torres Mamani, Edita
Villanueva Mori, Juan C.
Ousset, María J.
Medina, Víctor H.
Pérez Guerra, Uri H.
Huanca Mamani, Teodosio
author_sort Aisen, Eduardo G.
collection PubMed
description The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large protein component that has been implicated in the function of spermatozoa within the female reproductive tract. In this sense, the low fertility achieved using transcervical insemination with frozen-thawed spermatozoa in alpacas could be improved by adding SP. This study aimed to evaluate the effect of the whole SP on some in vitro parameters of alpaca spermatozoa after the freezing-thawing-process and the fertility after artificial insemination. It would contribute to a better understanding of the interaction between thawed sperm cells and SP. Spermatozoa were obtained by surgically diverted vas deferens. The samples were diluted with a Tris-based extender, packaged in straws, and frozen. At thawing, each straw was divided into two post-thawing conditions: with the addition of 10% of PBS (control) or with 10% SP (treatment). The sperm cells were evaluated using dynamic parameters, sperm cell morphology, and morphometry. Fertility was assessed by an artificial insemination trial. All in vitro parameters were analyzed by ANOVA. A heterogeneity test was scheduled for the fertility trial. After the freezing-thawing process, motility and plasma membrane functionality was improved when SP was added. No differences were found for post-thaw viability between the control and treatment samples. The percentage of normal cells was higher with SP at post-thawing, and a decrease of the presence of bent tailed spermatozoa with a droplet in the SP group was observed. The length of the head spermatozoa was 3.4% higher in the samples with PBS compared to those in which SP was added. Females pregnant at day 25 post-insemination were 0/12 (with SP inside the straw) and 1/10 (without SP inside the straw). In conclusion, the presence of 10% SP at post-thawing improves sperm cells' motility, functionality, and morphology, indicating that it would be beneficial to improve the frozen-thawed alpaca's physiology spermatozoa. More fertility trials must be developed to increase this knowledge.
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spelling pubmed-79027252021-02-25 Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing Aisen, Eduardo G. Huanca López, Wilfredo Pérez Durand, Manuel G. Torres Mamani, Edita Villanueva Mori, Juan C. Ousset, María J. Medina, Víctor H. Pérez Guerra, Uri H. Huanca Mamani, Teodosio Front Vet Sci Veterinary Science The viscous seminal plasma (SP) is currently a major impediment to the handling of ejaculate and the development of some biotechnologies in South American camelids. The vas deferens-collected spermatozoa of alpacas is a useful technique to avoid this problem. On the other hand, SP contains a large protein component that has been implicated in the function of spermatozoa within the female reproductive tract. In this sense, the low fertility achieved using transcervical insemination with frozen-thawed spermatozoa in alpacas could be improved by adding SP. This study aimed to evaluate the effect of the whole SP on some in vitro parameters of alpaca spermatozoa after the freezing-thawing-process and the fertility after artificial insemination. It would contribute to a better understanding of the interaction between thawed sperm cells and SP. Spermatozoa were obtained by surgically diverted vas deferens. The samples were diluted with a Tris-based extender, packaged in straws, and frozen. At thawing, each straw was divided into two post-thawing conditions: with the addition of 10% of PBS (control) or with 10% SP (treatment). The sperm cells were evaluated using dynamic parameters, sperm cell morphology, and morphometry. Fertility was assessed by an artificial insemination trial. All in vitro parameters were analyzed by ANOVA. A heterogeneity test was scheduled for the fertility trial. After the freezing-thawing process, motility and plasma membrane functionality was improved when SP was added. No differences were found for post-thaw viability between the control and treatment samples. The percentage of normal cells was higher with SP at post-thawing, and a decrease of the presence of bent tailed spermatozoa with a droplet in the SP group was observed. The length of the head spermatozoa was 3.4% higher in the samples with PBS compared to those in which SP was added. Females pregnant at day 25 post-insemination were 0/12 (with SP inside the straw) and 1/10 (without SP inside the straw). In conclusion, the presence of 10% SP at post-thawing improves sperm cells' motility, functionality, and morphology, indicating that it would be beneficial to improve the frozen-thawed alpaca's physiology spermatozoa. More fertility trials must be developed to increase this knowledge. Frontiers Media S.A. 2021-02-10 /pmc/articles/PMC7902725/ /pubmed/33644145 http://dx.doi.org/10.3389/fvets.2021.611301 Text en Copyright © 2021 Aisen, Huanca López, Pérez Durand, Torres Mamani, Villanueva Mori, Ousset, Medina, Pérez Guerra and Huanca Mamani. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Aisen, Eduardo G.
Huanca López, Wilfredo
Pérez Durand, Manuel G.
Torres Mamani, Edita
Villanueva Mori, Juan C.
Ousset, María J.
Medina, Víctor H.
Pérez Guerra, Uri H.
Huanca Mamani, Teodosio
Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing
title Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing
title_full Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing
title_fullStr Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing
title_full_unstemmed Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing
title_short Spermatozoa Obtained From Alpaca vas deferens. Effects of Seminal Plasma Added at Post-thawing
title_sort spermatozoa obtained from alpaca vas deferens. effects of seminal plasma added at post-thawing
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902725/
https://www.ncbi.nlm.nih.gov/pubmed/33644145
http://dx.doi.org/10.3389/fvets.2021.611301
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