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Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder

Inflammation and the gut-brain axis have been implicated in the pathogenesis of autism spectrum disorders (ASDs). To further understand the relationship between aberrant immune responses and dysbiotic features of the gut microbiome in ASD, we enrolled 45 ASD individuals and 41 healthy control subjec...

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Autores principales: Cao, Xia, Liu, Kevin, Liu, Jun, Liu, Yen-Wenn, Xu, Li, Wang, Hua, Zhu, Yunhui, Wang, Pengfei, Li, Zhiwei, Wen, Jie, Shen, Chen, Li, Meng, Nie, Zuqing, Kong, Xue-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902875/
https://www.ncbi.nlm.nih.gov/pubmed/33642989
http://dx.doi.org/10.3389/fnins.2021.635925
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author Cao, Xia
Liu, Kevin
Liu, Jun
Liu, Yen-Wenn
Xu, Li
Wang, Hua
Zhu, Yunhui
Wang, Pengfei
Li, Zhiwei
Wen, Jie
Shen, Chen
Li, Meng
Nie, Zuqing
Kong, Xue-Jun
author_facet Cao, Xia
Liu, Kevin
Liu, Jun
Liu, Yen-Wenn
Xu, Li
Wang, Hua
Zhu, Yunhui
Wang, Pengfei
Li, Zhiwei
Wen, Jie
Shen, Chen
Li, Meng
Nie, Zuqing
Kong, Xue-Jun
author_sort Cao, Xia
collection PubMed
description Inflammation and the gut-brain axis have been implicated in the pathogenesis of autism spectrum disorders (ASDs). To further understand the relationship between aberrant immune responses and dysbiotic features of the gut microbiome in ASD, we enrolled 45 ASD individuals and 41 healthy control subjects with ages ranging from 2 to 19 years. We found that ASD group subjects have significantly higher plasma levels of IL-2, IL-4, IL-5, IL-6, IL-10, TNF-α, TNF-β, and IFN-γ when compared to healthy controls (FDR-adjusted p < 0.05). The plasma levels of pro-inflammatory cytokines IFN-γ and IL-6 are found to be further associated with several largely pathogenic gut microbiota uniquely detected in subjects with ASD. Furthermore, the ASD gut microbiome is characterized by reduced levels of several beneficial microbiota, including Bacteroides (FDR-adjusted p < 0.01) and Lachnospiraceae (FDR-adjusted p < 0.001). Analysis of Lachnospiraceae family and genus level taxa suggested that relative abundances of such taxa are negatively correlated with pro-inflammatory signaling cytokines IFN-γ and IL-6, particularly in subjects with severe ASD as defined by CARS (p < 0.05). Several largely pathogenic genera are determined to be associated with the pro-inflammatory cytokines IFN-γ and IL-6 (FDR-adjusted p < 0.1). Additionally, IL-4 is significantly negatively correlated with CARS total score (p < 0.05). Based on such results, we propose that the association between the disturbances of specific cytokines and alterations in gut microbiota abundance observed in children and adolescents with ASD provides additional evidence on the induction of aberrant pro-inflammatory mechanisms in ASD and its early diagnosis.
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spelling pubmed-79028752021-02-25 Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder Cao, Xia Liu, Kevin Liu, Jun Liu, Yen-Wenn Xu, Li Wang, Hua Zhu, Yunhui Wang, Pengfei Li, Zhiwei Wen, Jie Shen, Chen Li, Meng Nie, Zuqing Kong, Xue-Jun Front Neurosci Neuroscience Inflammation and the gut-brain axis have been implicated in the pathogenesis of autism spectrum disorders (ASDs). To further understand the relationship between aberrant immune responses and dysbiotic features of the gut microbiome in ASD, we enrolled 45 ASD individuals and 41 healthy control subjects with ages ranging from 2 to 19 years. We found that ASD group subjects have significantly higher plasma levels of IL-2, IL-4, IL-5, IL-6, IL-10, TNF-α, TNF-β, and IFN-γ when compared to healthy controls (FDR-adjusted p < 0.05). The plasma levels of pro-inflammatory cytokines IFN-γ and IL-6 are found to be further associated with several largely pathogenic gut microbiota uniquely detected in subjects with ASD. Furthermore, the ASD gut microbiome is characterized by reduced levels of several beneficial microbiota, including Bacteroides (FDR-adjusted p < 0.01) and Lachnospiraceae (FDR-adjusted p < 0.001). Analysis of Lachnospiraceae family and genus level taxa suggested that relative abundances of such taxa are negatively correlated with pro-inflammatory signaling cytokines IFN-γ and IL-6, particularly in subjects with severe ASD as defined by CARS (p < 0.05). Several largely pathogenic genera are determined to be associated with the pro-inflammatory cytokines IFN-γ and IL-6 (FDR-adjusted p < 0.1). Additionally, IL-4 is significantly negatively correlated with CARS total score (p < 0.05). Based on such results, we propose that the association between the disturbances of specific cytokines and alterations in gut microbiota abundance observed in children and adolescents with ASD provides additional evidence on the induction of aberrant pro-inflammatory mechanisms in ASD and its early diagnosis. Frontiers Media S.A. 2021-02-10 /pmc/articles/PMC7902875/ /pubmed/33642989 http://dx.doi.org/10.3389/fnins.2021.635925 Text en Copyright © 2021 Cao, Liu, Liu, Liu, Xu, Wang, Zhu, Wang, Li, Wen, Shen, Li, Nie and Kong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Cao, Xia
Liu, Kevin
Liu, Jun
Liu, Yen-Wenn
Xu, Li
Wang, Hua
Zhu, Yunhui
Wang, Pengfei
Li, Zhiwei
Wen, Jie
Shen, Chen
Li, Meng
Nie, Zuqing
Kong, Xue-Jun
Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder
title Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder
title_full Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder
title_fullStr Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder
title_full_unstemmed Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder
title_short Dysbiotic Gut Microbiota and Dysregulation of Cytokine Profile in Children and Teens With Autism Spectrum Disorder
title_sort dysbiotic gut microbiota and dysregulation of cytokine profile in children and teens with autism spectrum disorder
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902875/
https://www.ncbi.nlm.nih.gov/pubmed/33642989
http://dx.doi.org/10.3389/fnins.2021.635925
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